Strain Fitness in Escherichia coli BW25113 around b2356

Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfdK and yfdL are separated by 26 nucleotidesyfdL and yfdM overlap by 5 nucleotidesyfdM and yfdN overlap by 1 nucleotidesyfdN and oweS overlap by 4 nucleotides b2354: yfdK - CPS-53 (KpLE1) prophage; conserved protein (NCBI), at 2,469,099 to 2,469,539 yfdK b2355: yfdL - CPS-53 (KpLE1) prophage; conserved protein (RefSeq), at 2,469,566 to 2,470,144 yfdL b2356: yfdM - orf, hypothetical protein (VIMSS), at 2,470,140 to 2,470,409 yfdM b2357: yfdN - CPS-53 (KpLE1) prophage; predicted protein (NCBI), at 2,470,409 to 2,470,903 yfdN b2358: oweS - CPS-53 (KpLE1) prophage; predicted defective phage replication protein O (RefSeq), at 2,470,900 to 2,471,607 oweS Position (kb) 2470 2471Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2469.164 kb on + strand, within yfdKat 2469.164 kb on + strand, within yfdKat 2469.164 kb on + strand, within yfdKat 2469.164 kb on + strand, within yfdKat 2469.164 kb on + strand, within yfdKat 2469.164 kb on + strand, within yfdKat 2469.164 kb on + strand, within yfdKat 2469.164 kb on + strand, within yfdKat 2469.170 kb on - strand, within yfdKat 2469.170 kb on - strand, within yfdKat 2469.172 kb on - strand, within yfdKat 2469.179 kb on + strand, within yfdKat 2469.210 kb on + strand, within yfdKat 2469.210 kb on + strand, within yfdKat 2469.236 kb on - strand, within yfdKat 2469.241 kb on - strand, within yfdKat 2469.331 kb on - strand, within yfdKat 2469.331 kb on - strand, within yfdKat 2469.331 kb on - strand, within yfdKat 2469.332 kb on - strand, within yfdKat 2469.332 kb on - strand, within yfdKat 2469.332 kb on - strand, within yfdKat 2469.332 kb on - strand, within yfdKat 2469.354 kb on - strand, within yfdKat 2469.442 kb on - strand, within yfdKat 2469.442 kb on - strand, within yfdKat 2469.451 kb on - strand, within yfdKat 2469.457 kb on + strand, within yfdKat 2469.457 kb on + strand, within yfdKat 2469.459 kb on + strand, within yfdKat 2469.460 kb on - strand, within yfdKat 2469.461 kb on - strand, within yfdKat 2469.461 kb on - strand, within yfdKat 2469.463 kb on + strand, within yfdKat 2469.596 kb on + strandat 2469.596 kb on + strandat 2469.610 kb on - strandat 2469.626 kb on - strand, within yfdLat 2469.659 kb on - strand, within yfdLat 2469.663 kb on - strand, within yfdLat 2469.663 kb on - strand, within yfdLat 2469.685 kb on + strand, within yfdLat 2469.688 kb on - strand, within yfdLat 2469.695 kb on - strand, within yfdLat 2469.751 kb on - strand, within yfdLat 2469.754 kb on - strand, within yfdLat 2469.758 kb on - strand, within yfdLat 2469.763 kb on - strand, within yfdLat 2469.763 kb on - strand, within yfdLat 2469.763 kb on - strand, within yfdLat 2469.763 kb on - strand, within yfdLat 2469.763 kb on - strand, within yfdLat 2469.763 kb on - strand, within yfdLat 2469.772 kb on + strand, within yfdLat 2469.801 kb on - strand, within yfdLat 2469.852 kb on - strand, within yfdLat 2469.852 kb on - strand, within yfdLat 2469.857 kb on + strand, within yfdLat 2469.865 kb on + strand, within yfdLat 2469.867 kb on - strand, within yfdLat 2469.890 kb on - strand, within yfdLat 2469.940 kb on - strand, within yfdLat 2469.942 kb on - strand, within yfdLat 2469.942 kb on - strand, within yfdLat 2469.998 kb on + strand, within yfdLat 2470.038 kb on - strand, within yfdLat 2470.038 kb on - strand, within yfdLat 2470.049 kb on - strand, within yfdLat 2470.049 kb on - strand, within yfdLat 2470.090 kb on - strandat 2470.168 kb on + strand, within yfdMat 2470.211 kb on - strand, within yfdMat 2470.448 kb on + strandat 2470.448 kb on + strandat 2470.477 kb on + strand, within yfdNat 2470.477 kb on + strand, within yfdNat 2470.484 kb on - strand, within yfdNat 2470.484 kb on - strand, within yfdNat 2470.741 kb on - strand, within yfdNat 2470.913 kb on - strandat 2470.928 kb on + strandat 2471.021 kb on - strand, within oweSat 2471.021 kb on - strand, within oweSat 2471.090 kb on + strand, within oweSat 2471.133 kb on + strand, within oweSat 2471.133 kb on + strand, within oweSat 2471.201 kb on + strand, within oweSat 2471.201 kb on + strand, within oweSat 2471.263 kb on + strand, within oweSat 2471.277 kb on - strand, within oweSat 2471.277 kb on - strand, within oweSat 2471.372 kb on + strand, within oweSat 2471.372 kb on + strand, within oweS

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
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2,469,164 + yfdK b2354 0.15 +2.0
2,469,164 + yfdK b2354 0.15 +1.0
2,469,164 + yfdK b2354 0.15 +1.0
2,469,164 + yfdK b2354 0.15 +2.0
2,469,164 + yfdK b2354 0.15 +0.4
2,469,164 + yfdK b2354 0.15 -0.0
2,469,164 + yfdK b2354 0.15 +0.4
2,469,164 + yfdK b2354 0.15 +1.0
2,469,170 - yfdK b2354 0.16 -0.3
2,469,170 - yfdK b2354 0.16 -0.5
2,469,172 - yfdK b2354 0.17 -0.4
2,469,179 + yfdK b2354 0.18 +1.0
2,469,210 + yfdK b2354 0.25 +2.0
2,469,210 + yfdK b2354 0.25 +2.0
2,469,236 - yfdK b2354 0.31 +1.1
2,469,241 - yfdK b2354 0.32 -1.1
2,469,331 - yfdK b2354 0.53 -1.3
2,469,331 - yfdK b2354 0.53 +2.0
2,469,331 - yfdK b2354 0.53 -1.2
2,469,332 - yfdK b2354 0.53 -1.7
2,469,332 - yfdK b2354 0.53 -0.7
2,469,332 - yfdK b2354 0.53 -0.6
2,469,332 - yfdK b2354 0.53 +3.0
2,469,354 - yfdK b2354 0.58 +1.0
2,469,442 - yfdK b2354 0.78 +0.8
2,469,442 - yfdK b2354 0.78 +0.2
2,469,451 - yfdK b2354 0.80 -0.3
2,469,457 + yfdK b2354 0.81 +0.4
2,469,457 + yfdK b2354 0.81 +1.0
2,469,459 + yfdK b2354 0.82 +1.0
2,469,460 - yfdK b2354 0.82 +1.0
2,469,461 - yfdK b2354 0.82 -0.0
2,469,461 - yfdK b2354 0.82 +1.4
2,469,463 + yfdK b2354 0.83 +2.0
2,469,596 + +2.0
2,469,596 + +2.0
2,469,610 - -0.3
2,469,626 - yfdL b2355 0.10 +0.8
2,469,659 - yfdL b2355 0.16 +0.2
2,469,663 - yfdL b2355 0.17 -0.8
2,469,663 - yfdL b2355 0.17 +0.5
2,469,685 + yfdL b2355 0.21 +2.0
2,469,688 - yfdL b2355 0.21 -1.7
2,469,695 - yfdL b2355 0.22 -0.2
2,469,751 - yfdL b2355 0.32 +0.2
2,469,754 - yfdL b2355 0.32 -1.0
2,469,758 - yfdL b2355 0.33 +0.4
2,469,763 - yfdL b2355 0.34 +1.0
2,469,763 - yfdL b2355 0.34 +0.8
2,469,763 - yfdL b2355 0.34 -0.4
2,469,763 - yfdL b2355 0.34 -0.3
2,469,763 - yfdL b2355 0.34 +0.1
2,469,763 - yfdL b2355 0.34 +1.4
2,469,772 + yfdL b2355 0.36 +2.0
2,469,801 - yfdL b2355 0.41 -0.1
2,469,852 - yfdL b2355 0.49 +0.6
2,469,852 - yfdL b2355 0.49 -0.0
2,469,857 + yfdL b2355 0.50 +2.0
2,469,865 + yfdL b2355 0.52 +1.0
2,469,867 - yfdL b2355 0.52 +0.2
2,469,890 - yfdL b2355 0.56 +0.8
2,469,940 - yfdL b2355 0.65 +0.3
2,469,942 - yfdL b2355 0.65 -0.7
2,469,942 - yfdL b2355 0.65 -0.7
2,469,998 + yfdL b2355 0.75 +0.4
2,470,038 - yfdL b2355 0.82 -1.8
2,470,038 - yfdL b2355 0.82 +0.7
2,470,049 - yfdL b2355 0.83 -0.8
2,470,049 - yfdL b2355 0.83 -0.4
2,470,090 - -0.5
2,470,168 + yfdM b2356 0.10 +0.8
2,470,211 - yfdM b2356 0.26 +0.5
2,470,448 + +0.4
2,470,448 + -0.0
2,470,477 + yfdN b2357 0.14 +0.7
2,470,477 + yfdN b2357 0.14 +0.6
2,470,484 - yfdN b2357 0.15 +0.4
2,470,484 - yfdN b2357 0.15 -0.0
2,470,741 - yfdN b2357 0.67 +0.4
2,470,913 - +0.9
2,470,928 + -0.2
2,471,021 - oweS b2358 0.17 +0.5
2,471,021 - oweS b2358 0.17 +0.8
2,471,090 + oweS b2358 0.27 -0.4
2,471,133 + oweS b2358 0.33 +0.2
2,471,133 + oweS b2358 0.33 -0.4
2,471,201 + oweS b2358 0.43 +0.3
2,471,201 + oweS b2358 0.43 -0.7
2,471,263 + oweS b2358 0.51 -1.7
2,471,277 - oweS b2358 0.53 -0.3
2,471,277 - oweS b2358 0.53 -0.3
2,471,372 + oweS b2358 0.67 -0.0
2,471,372 + oweS b2358 0.67 -0.0

Or see this region's nucleotide sequence