Strain Fitness in Escherichia coli BW25113 around b1712

Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntbtuC and ihfA are separated by 100 nucleotidesihfA and pheT are separated by 4 nucleotides b1711: btuC - vtamin B12-transporter permease (NCBI), at 1,792,196 to 1,793,176 btuC b1712: ihfA - integration host factor subunit alpha (NCBI), at 1,793,277 to 1,793,576 ihfA b1713: pheT - phenylalanyl-tRNA synthetase beta subunit (NCBI), at 1,793,581 to 1,795,968 pheT Position (kb) 1793 1794Strain fitness (log2 ratio) -1 0 1 2at 1792.288 kb on - strandat 1792.295 kb on + strand, within btuCat 1792.295 kb on + strand, within btuCat 1792.423 kb on - strand, within btuCat 1792.432 kb on + strand, within btuCat 1792.492 kb on + strand, within btuCat 1792.566 kb on + strand, within btuCat 1792.592 kb on - strand, within btuCat 1792.783 kb on + strand, within btuCat 1792.820 kb on - strand, within btuCat 1792.846 kb on + strand, within btuCat 1792.980 kb on + strand, within btuCat 1793.064 kb on + strand, within btuCat 1793.108 kb on + strandat 1793.167 kb on + strandat 1793.280 kb on - strandat 1793.330 kb on + strand, within ihfAat 1793.353 kb on + strand, within ihfAat 1793.417 kb on + strand, within ihfAat 1793.533 kb on - strand, within ihfA

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
remove
1,792,288 - -0.6
1,792,295 + btuC b1711 0.10 +0.4
1,792,295 + btuC b1711 0.10 +1.2
1,792,423 - btuC b1711 0.23 -0.4
1,792,432 + btuC b1711 0.24 -1.3
1,792,492 + btuC b1711 0.30 -0.8
1,792,566 + btuC b1711 0.38 +0.7
1,792,592 - btuC b1711 0.40 -0.3
1,792,783 + btuC b1711 0.60 -0.5
1,792,820 - btuC b1711 0.64 -0.9
1,792,846 + btuC b1711 0.66 -0.5
1,792,980 + btuC b1711 0.80 +0.2
1,793,064 + btuC b1711 0.88 -0.4
1,793,108 + -0.8
1,793,167 + -0.3
1,793,280 - +0.3
1,793,330 + ihfA b1712 0.18 +1.9
1,793,353 + ihfA b1712 0.25 +1.9
1,793,417 + ihfA b1712 0.47 +1.9
1,793,533 - ihfA b1712 0.85 +1.9

Or see this region's nucleotide sequence