Strain Fitness in Escherichia coli BW25113 around b0991

Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcspH and cspG are separated by 285 nucleotidescspG and ymcE are separated by 173 nucleotidesymcE and gnsA overlap by 11 nucleotidesgnsA and yccM are separated by 48 nucleotides b0989: cspH - stress protein, member of the CspA-family (NCBI), at 1,050,186 to 1,050,398 cspH b0990: cspG - DNA-binding transcriptional regulator (NCBI), at 1,050,684 to 1,050,896 cspG b0991: ymcE - cold shock gene (NCBI), at 1,051,070 to 1,051,300 ymcE b4517: gnsA - predicted regulator of phosphatidylethanolamine synthesis (NCBI), at 1,051,290 to 1,051,463 gnsA b0992: yccM - predicted 4Fe-4S membrane protein (NCBI), at 1,051,512 to 1,052,585 yccM Position (kb) 1051 1052Strain fitness (log2 ratio) -1 0 1 2at 1050.149 kb on + strandat 1050.154 kb on + strandat 1050.164 kb on + strandat 1050.180 kb on + strandat 1050.180 kb on + strandat 1050.184 kb on + strandat 1050.184 kb on + strandat 1050.184 kb on + strandat 1050.184 kb on + strandat 1050.194 kb on + strandat 1050.194 kb on + strandat 1050.211 kb on + strand, within cspHat 1050.211 kb on + strand, within cspHat 1050.226 kb on + strand, within cspHat 1050.254 kb on + strand, within cspHat 1050.254 kb on + strand, within cspHat 1050.269 kb on + strand, within cspHat 1050.269 kb on + strand, within cspHat 1050.277 kb on - strand, within cspHat 1050.277 kb on - strand, within cspHat 1050.336 kb on + strand, within cspHat 1050.349 kb on + strand, within cspHat 1050.398 kb on + strandat 1050.398 kb on + strandat 1050.435 kb on - strandat 1050.435 kb on - strandat 1050.516 kb on + strandat 1050.565 kb on - strandat 1050.590 kb on + strandat 1050.613 kb on + strandat 1050.613 kb on + strandat 1050.733 kb on + strand, within cspGat 1050.756 kb on - strand, within cspGat 1050.756 kb on - strand, within cspGat 1050.883 kb on + strandat 1050.944 kb on + strandat 1050.944 kb on + strandat 1050.949 kb on + strandat 1050.949 kb on + strandat 1050.949 kb on + strandat 1050.955 kb on - strandat 1050.957 kb on - strandat 1050.970 kb on - strandat 1051.100 kb on - strand, within ymcEat 1051.100 kb on - strand, within ymcEat 1051.107 kb on + strand, within ymcEat 1051.107 kb on + strand, within ymcEat 1051.149 kb on + strand, within ymcEat 1051.149 kb on + strand, within ymcEat 1051.151 kb on + strand, within ymcEat 1051.161 kb on - strand, within ymcEat 1051.161 kb on - strand, within ymcEat 1051.203 kb on + strand, within ymcEat 1051.203 kb on + strand, within ymcEat 1051.228 kb on + strand, within ymcEat 1051.255 kb on - strand, within ymcEat 1051.480 kb on - strandat 1051.591 kb on - strandat 1051.631 kb on - strand, within yccMat 1051.640 kb on + strand, within yccMat 1051.714 kb on - strand, within yccMat 1051.717 kb on + strand, within yccMat 1051.735 kb on - strand, within yccMat 1051.770 kb on - strand, within yccMat 1051.872 kb on - strand, within yccMat 1051.923 kb on + strand, within yccMat 1051.923 kb on + strand, within yccMat 1051.940 kb on - strand, within yccMat 1051.968 kb on + strand, within yccMat 1052.009 kb on + strand, within yccMat 1052.029 kb on + strand, within yccMat 1052.073 kb on + strand, within yccMat 1052.124 kb on - strand, within yccMat 1052.175 kb on + strand, within yccM

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
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1,050,149 + +0.3
1,050,154 + +0.4
1,050,164 + -0.9
1,050,180 + +0.4
1,050,180 + -1.3
1,050,184 + -0.5
1,050,184 + -0.3
1,050,184 + -0.6
1,050,184 + +1.4
1,050,194 + +0.1
1,050,194 + -1.3
1,050,211 + cspH b0989 0.12 -0.1
1,050,211 + cspH b0989 0.12 +1.2
1,050,226 + cspH b0989 0.19 +1.9
1,050,254 + cspH b0989 0.32 +0.3
1,050,254 + cspH b0989 0.32 +0.4
1,050,269 + cspH b0989 0.39 -0.1
1,050,269 + cspH b0989 0.39 +1.9
1,050,277 - cspH b0989 0.43 +1.9
1,050,277 - cspH b0989 0.43 +1.9
1,050,336 + cspH b0989 0.70 +0.9
1,050,349 + cspH b0989 0.77 +1.9
1,050,398 + +1.9
1,050,398 + +1.9
1,050,435 - +1.9
1,050,435 - +1.9
1,050,516 + +1.9
1,050,565 - +1.9
1,050,590 + +0.9
1,050,613 + -0.2
1,050,613 + +0.3
1,050,733 + cspG b0990 0.23 -0.5
1,050,756 - cspG b0990 0.34 -0.1
1,050,756 - cspG b0990 0.34 -1.0
1,050,883 + +0.1
1,050,944 + -0.8
1,050,944 + +2.0
1,050,949 + -0.0
1,050,949 + -0.6
1,050,949 + +0.9
1,050,955 - -0.1
1,050,957 - -0.4
1,050,970 - +0.0
1,051,100 - ymcE b0991 0.13 +0.5
1,051,100 - ymcE b0991 0.13 -0.5
1,051,107 + ymcE b0991 0.16 +0.3
1,051,107 + ymcE b0991 0.16 +1.3
1,051,149 + ymcE b0991 0.34 +1.1
1,051,149 + ymcE b0991 0.34 +1.9
1,051,151 + ymcE b0991 0.35 -0.9
1,051,161 - ymcE b0991 0.39 -0.6
1,051,161 - ymcE b0991 0.39 +0.3
1,051,203 + ymcE b0991 0.58 +1.2
1,051,203 + ymcE b0991 0.58 +1.5
1,051,228 + ymcE b0991 0.68 +1.3
1,051,255 - ymcE b0991 0.80 +0.6
1,051,480 - +0.3
1,051,591 - -0.5
1,051,631 - yccM b0992 0.11 -0.3
1,051,640 + yccM b0992 0.12 +0.9
1,051,714 - yccM b0992 0.19 +0.6
1,051,717 + yccM b0992 0.19 +0.4
1,051,735 - yccM b0992 0.21 +0.5
1,051,770 - yccM b0992 0.24 +0.8
1,051,872 - yccM b0992 0.34 -0.4
1,051,923 + yccM b0992 0.38 +0.3
1,051,923 + yccM b0992 0.38 -0.6
1,051,940 - yccM b0992 0.40 +1.6
1,051,968 + yccM b0992 0.42 +0.5
1,052,009 + yccM b0992 0.46 +1.1
1,052,029 + yccM b0992 0.48 -0.7
1,052,073 + yccM b0992 0.52 -1.7
1,052,124 - yccM b0992 0.57 -0.0
1,052,175 + yccM b0992 0.62 +0.1

Or see this region's nucleotide sequence