Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt focA and ycaO are separated by 405 nucleotides ycaO and ycaP are separated by 129 nucleotides
b0904: focA - formate transporter (NCBI), at 952,832 to 953,689
focA
b0905: ycaO - orf, hypothetical protein (VIMSS), at 954,095 to 955,855
ycaO
b0906: ycaP - conserved inner membrane protein (NCBI), at 955,985 to 956,677
ycaP
Position (kb)
954
955
956 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 953.096 kb on + strand, within focA at 953.155 kb on - strand, within focA at 953.163 kb on - strand, within focA at 953.175 kb on + strand, within focA at 953.175 kb on + strand, within focA at 953.193 kb on + strand, within focA at 953.246 kb on + strand, within focA at 953.423 kb on - strand, within focA at 953.423 kb on - strand, within focA at 953.514 kb on + strand, within focA at 953.516 kb on - strand, within focA at 953.516 kb on - strand, within focA at 953.557 kb on + strand, within focA at 953.566 kb on - strand, within focA at 953.581 kb on + strand, within focA at 953.610 kb on + strand at 953.611 kb on + strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.659 kb on + strand at 953.682 kb on + strand at 953.682 kb on + strand at 953.691 kb on - strand at 953.697 kb on - strand at 953.774 kb on + strand at 953.774 kb on + strand at 953.774 kb on + strand at 953.892 kb on + strand at 953.916 kb on + strand at 953.972 kb on + strand at 954.045 kb on + strand at 954.064 kb on - strand at 954.068 kb on + strand at 954.072 kb on + strand at 954.073 kb on + strand at 954.073 kb on + strand at 954.076 kb on - strand at 954.076 kb on - strand at 954.076 kb on - strand at 954.076 kb on - strand at 954.076 kb on - strand at 954.081 kb on - strand at 954.081 kb on - strand at 954.085 kb on - strand at 954.124 kb on + strand at 954.138 kb on - strand at 954.147 kb on + strand at 954.147 kb on + strand at 954.158 kb on + strand at 954.172 kb on + strand at 954.172 kb on + strand at 954.188 kb on + strand at 954.188 kb on + strand at 954.240 kb on + strand at 954.240 kb on + strand at 954.258 kb on + strand at 954.260 kb on + strand at 954.260 kb on + strand at 954.263 kb on - strand at 954.318 kb on + strand, within ycaO at 954.394 kb on - strand, within ycaO at 954.394 kb on - strand, within ycaO at 954.402 kb on + strand, within ycaO at 954.402 kb on + strand, within ycaO at 954.402 kb on + strand, within ycaO at 954.410 kb on - strand, within ycaO at 954.418 kb on + strand, within ycaO at 954.426 kb on - strand, within ycaO at 954.426 kb on - strand, within ycaO at 954.458 kb on - strand, within ycaO at 954.483 kb on + strand, within ycaO at 954.491 kb on - strand, within ycaO at 954.637 kb on + strand, within ycaO at 954.707 kb on - strand, within ycaO at 954.717 kb on + strand, within ycaO at 954.741 kb on + strand, within ycaO at 954.741 kb on + strand, within ycaO at 954.762 kb on + strand, within ycaO at 954.764 kb on + strand, within ycaO at 954.779 kb on - strand, within ycaO at 954.789 kb on - strand, within ycaO at 954.854 kb on - strand, within ycaO at 954.888 kb on - strand, within ycaO at 954.889 kb on + strand, within ycaO at 954.918 kb on - strand, within ycaO at 954.952 kb on - strand, within ycaO at 955.067 kb on + strand, within ycaO at 955.067 kb on + strand, within ycaO at 955.070 kb on - strand, within ycaO at 955.070 kb on - strand, within ycaO at 955.070 kb on - strand, within ycaO at 955.080 kb on - strand, within ycaO at 955.092 kb on - strand, within ycaO at 955.284 kb on - strand, within ycaO at 955.579 kb on + strand, within ycaO at 955.611 kb on + strand, within ycaO at 955.611 kb on + strand, within ycaO at 955.611 kb on + strand, within ycaO at 955.619 kb on - strand, within ycaO at 955.688 kb on + strand at 955.724 kb on - strand at 955.755 kb on - strand at 955.755 kb on - strand at 955.771 kb on - strand at 955.870 kb on - strand at 955.952 kb on - strand at 955.952 kb on - strand at 956.020 kb on - strand at 956.051 kb on + strand at 956.051 kb on + strand at 956.063 kb on + strand, within ycaP at 956.070 kb on + strand, within ycaP at 956.078 kb on - strand, within ycaP at 956.123 kb on - strand, within ycaP at 956.151 kb on + strand, within ycaP at 956.151 kb on + strand, within ycaP at 956.185 kb on + strand, within ycaP at 956.250 kb on - strand, within ycaP at 956.253 kb on + strand, within ycaP at 956.253 kb on + strand, within ycaP at 956.260 kb on - strand, within ycaP at 956.260 kb on - strand, within ycaP at 956.390 kb on - strand, within ycaP at 956.391 kb on + strand, within ycaP at 956.391 kb on + strand, within ycaP at 956.453 kb on + strand, within ycaP at 956.523 kb on + strand, within ycaP at 956.523 kb on + strand, within ycaP at 956.532 kb on + strand, within ycaP at 956.532 kb on + strand, within ycaP at 956.772 kb on + strand at 956.774 kb on + strand at 956.774 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM remove 953,096 + focA b0904 0.31 +0.0 953,155 - focA b0904 0.38 -0.8 953,163 - focA b0904 0.39 -1.1 953,175 + focA b0904 0.40 -0.5 953,175 + focA b0904 0.40 -1.6 953,193 + focA b0904 0.42 +0.1 953,246 + focA b0904 0.48 +0.2 953,423 - focA b0904 0.69 +1.6 953,423 - focA b0904 0.69 -0.2 953,514 + focA b0904 0.79 -1.5 953,516 - focA b0904 0.80 +0.7 953,516 - focA b0904 0.80 -0.5 953,557 + focA b0904 0.84 -2.0 953,566 - focA b0904 0.86 +0.9 953,581 + focA b0904 0.87 +0.5 953,610 + +0.3 953,611 + -0.9 953,618 - -1.9 953,618 - +0.5 953,618 - -1.8 953,618 - -0.4 953,618 - -0.2 953,618 - +0.3 953,618 - +0.5 953,618 - +0.9 953,618 - +0.9 953,659 + -1.5 953,682 + +1.1 953,682 + -0.5 953,691 - +0.9 953,697 - +1.3 953,774 + +0.6 953,774 + +1.4 953,774 + -0.2 953,892 + +0.7 953,916 + +0.6 953,972 + -0.1 954,045 + -1.3 954,064 - -1.4 954,068 + +0.9 954,072 + +1.9 954,073 + +0.9 954,073 + +1.9 954,076 - +1.3 954,076 - -0.0 954,076 - +0.5 954,076 - +2.2 954,076 - -0.4 954,081 - +1.3 954,081 - +0.1 954,085 - +0.9 954,124 + +0.4 954,138 - -0.7 954,147 + +0.3 954,147 + -0.1 954,158 + -1.1 954,172 + +3.2 954,172 + -0.5 954,188 + +0.3 954,188 + +0.7 954,240 + -1.8 954,240 + -0.4 954,258 + -2.4 954,260 + -0.2 954,260 + +1.3 954,263 - -2.0 954,318 + ycaO b0905 0.13 +0.9 954,394 - ycaO b0905 0.17 -1.1 954,394 - ycaO b0905 0.17 +0.1 954,402 + ycaO b0905 0.17 -0.1 954,402 + ycaO b0905 0.17 -0.7 954,402 + ycaO b0905 0.17 +0.9 954,410 - ycaO b0905 0.18 +0.9 954,418 + ycaO b0905 0.18 +0.3 954,426 - ycaO b0905 0.19 -2.2 954,426 - ycaO b0905 0.19 -0.8 954,458 - ycaO b0905 0.21 -0.4 954,483 + ycaO b0905 0.22 +0.3 954,491 - ycaO b0905 0.22 +0.3 954,637 + ycaO b0905 0.31 -0.9 954,707 - ycaO b0905 0.35 +0.3 954,717 + ycaO b0905 0.35 +0.1 954,741 + ycaO b0905 0.37 -1.2 954,741 + ycaO b0905 0.37 -0.9 954,762 + ycaO b0905 0.38 -1.5 954,764 + ycaO b0905 0.38 +0.6 954,779 - ycaO b0905 0.39 -0.8 954,789 - ycaO b0905 0.39 +0.1 954,854 - ycaO b0905 0.43 -2.8 954,888 - ycaO b0905 0.45 -1.0 954,889 + ycaO b0905 0.45 +0.1 954,918 - ycaO b0905 0.47 -0.3 954,952 - ycaO b0905 0.49 -0.7 955,067 + ycaO b0905 0.55 -0.2 955,067 + ycaO b0905 0.55 -1.5 955,070 - ycaO b0905 0.55 -0.1 955,070 - ycaO b0905 0.55 +0.2 955,070 - ycaO b0905 0.55 -0.4 955,080 - ycaO b0905 0.56 +0.9 955,092 - ycaO b0905 0.57 -0.5 955,284 - ycaO b0905 0.68 -0.1 955,579 + ycaO b0905 0.84 -1.2 955,611 + ycaO b0905 0.86 -1.5 955,611 + ycaO b0905 0.86 -0.8 955,611 + ycaO b0905 0.86 -1.0 955,619 - ycaO b0905 0.87 -1.7 955,688 + -2.1 955,724 - -1.4 955,755 - -0.5 955,755 - -0.9 955,771 - -0.8 955,870 - +1.6 955,952 - +0.2 955,952 - -1.1 956,020 - +0.6 956,051 + -0.4 956,051 + -3.0 956,063 + ycaP b0906 0.11 -0.1 956,070 + ycaP b0906 0.12 -0.7 956,078 - ycaP b0906 0.13 -0.2 956,123 - ycaP b0906 0.20 -2.8 956,151 + ycaP b0906 0.24 -1.2 956,151 + ycaP b0906 0.24 -0.1 956,185 + ycaP b0906 0.29 +0.7 956,250 - ycaP b0906 0.38 +0.3 956,253 + ycaP b0906 0.39 -1.6 956,253 + ycaP b0906 0.39 +0.2 956,260 - ycaP b0906 0.40 -0.1 956,260 - ycaP b0906 0.40 -0.5 956,390 - ycaP b0906 0.58 -0.8 956,391 + ycaP b0906 0.59 -0.4 956,391 + ycaP b0906 0.59 -0.0 956,453 + ycaP b0906 0.68 +0.5 956,523 + ycaP b0906 0.78 -0.2 956,523 + ycaP b0906 0.78 -1.2 956,532 + ycaP b0906 0.79 +0.0 956,532 + ycaP b0906 0.79 -0.8 956,772 + -0.7 956,774 + +0.4 956,774 + +0.4
Or see this region's nucleotide sequence