Strain Fitness in Escherichia coli BW25113 around b0796

Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybhF and ybhG overlap by 8 nucleotidesybhG and ybiH overlap by 1 nucleotidesybiH and rhlE are separated by 228 nucleotides b0794: ybhF - putative ATP-binding component of a transport system (VIMSS), at 826,468 to 828,204 ybhF b0795: ybhG - hypothetical protein (NCBI), at 828,197 to 829,195 ybhG b0796: ybiH - putative transcriptional regulator (VIMSS), at 829,195 to 829,866 ybiH b0797: rhlE - RNA helicase (NCBI), at 830,095 to 831,459 rhlE Position (kb) 829 830Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 828.197 kb on + strandat 828.197 kb on + strandat 828.205 kb on - strandat 828.207 kb on - strandat 828.311 kb on - strand, within ybhGat 828.410 kb on - strand, within ybhGat 828.469 kb on + strand, within ybhGat 828.502 kb on - strand, within ybhGat 828.504 kb on + strand, within ybhGat 828.528 kb on - strand, within ybhGat 828.528 kb on - strand, within ybhGat 828.620 kb on - strand, within ybhGat 828.725 kb on - strand, within ybhGat 828.876 kb on + strand, within ybhGat 828.906 kb on - strand, within ybhGat 828.949 kb on - strand, within ybhGat 828.953 kb on + strand, within ybhGat 828.959 kb on - strand, within ybhGat 828.959 kb on - strand, within ybhGat 828.978 kb on + strand, within ybhGat 828.978 kb on + strand, within ybhGat 829.047 kb on - strandat 829.088 kb on - strand, within ybhGat 829.093 kb on - strand, within ybhGat 829.295 kb on - strand, within ybiHat 829.360 kb on + strand, within ybiHat 829.360 kb on + strand, within ybiHat 829.535 kb on - strand, within ybiHat 829.598 kb on + strand, within ybiHat 829.679 kb on + strand, within ybiHat 829.841 kb on - strandat 829.841 kb on - strandat 829.845 kb on - strandat 829.858 kb on + strandat 829.996 kb on + strandat 830.051 kb on + strandat 830.059 kb on + strandat 830.059 kb on + strandat 830.123 kb on + strandat 830.123 kb on + strandat 830.123 kb on + strandat 830.146 kb on + strandat 830.156 kb on + strandat 830.159 kb on - strandat 830.176 kb on - strandat 830.182 kb on + strandat 830.182 kb on + strandat 830.192 kb on - strandat 830.249 kb on - strand, within rhlEat 830.277 kb on - strand, within rhlEat 830.277 kb on - strand, within rhlEat 830.333 kb on + strand, within rhlEat 830.333 kb on + strand, within rhlEat 830.335 kb on - strand, within rhlEat 830.335 kb on - strand, within rhlEat 830.341 kb on - strand, within rhlEat 830.390 kb on + strand, within rhlEat 830.531 kb on + strand, within rhlEat 830.836 kb on + strand, within rhlEat 830.850 kb on - strand, within rhlEat 830.860 kb on - strand, within rhlEat 830.860 kb on - strand, within rhlE

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
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828,197 + -0.0
828,197 + +0.8
828,205 - +0.2
828,207 - +0.2
828,311 - ybhG b0795 0.11 -0.9
828,410 - ybhG b0795 0.21 -0.5
828,469 + ybhG b0795 0.27 -0.1
828,502 - ybhG b0795 0.31 +0.3
828,504 + ybhG b0795 0.31 +0.5
828,528 - ybhG b0795 0.33 -2.8
828,528 - ybhG b0795 0.33 -1.0
828,620 - ybhG b0795 0.42 +0.6
828,725 - ybhG b0795 0.53 +0.8
828,876 + ybhG b0795 0.68 -1.0
828,906 - ybhG b0795 0.71 +0.7
828,949 - ybhG b0795 0.75 +1.0
828,953 + ybhG b0795 0.76 +2.0
828,959 - ybhG b0795 0.76 +0.0
828,959 - ybhG b0795 0.76 -0.5
828,978 + ybhG b0795 0.78 -0.2
828,978 + ybhG b0795 0.78 -0.7
829,047 - -0.0
829,088 - ybhG b0795 0.89 -0.5
829,093 - ybhG b0795 0.90 -0.0
829,295 - ybiH b0796 0.15 +0.9
829,360 + ybiH b0796 0.25 -0.1
829,360 + ybiH b0796 0.25 +0.9
829,535 - ybiH b0796 0.51 +0.4
829,598 + ybiH b0796 0.60 +0.9
829,679 + ybiH b0796 0.72 +0.8
829,841 - +0.0
829,841 - +0.8
829,845 - -1.0
829,858 + +2.3
829,996 + -0.6
830,051 + +1.2
830,059 + -1.2
830,059 + +1.2
830,123 + +0.9
830,123 + -0.0
830,123 + +1.0
830,146 + -0.0
830,156 + +0.1
830,159 - +0.8
830,176 - +0.1
830,182 + +0.9
830,182 + +0.1
830,192 - +0.4
830,249 - rhlE b0797 0.11 +0.8
830,277 - rhlE b0797 0.13 +1.2
830,277 - rhlE b0797 0.13 -0.7
830,333 + rhlE b0797 0.17 +0.2
830,333 + rhlE b0797 0.17 -1.3
830,335 - rhlE b0797 0.18 +0.8
830,335 - rhlE b0797 0.18 -0.9
830,341 - rhlE b0797 0.18 +1.2
830,390 + rhlE b0797 0.22 -1.0
830,531 + rhlE b0797 0.32 +0.1
830,836 + rhlE b0797 0.54 -0.4
830,850 - rhlE b0797 0.55 -0.2
830,860 - rhlE b0797 0.56 -1.3
830,860 - rhlE b0797 0.56 +0.4

Or see this region's nucleotide sequence