Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybbM and ybbN are separated by 62 nucleotides ybbN and ybbO are separated by 60 nucleotides ybbO and tesA overlap by 11 nucleotides tesA and ybbA overlap by 33 nucleotides
b0491: ybbM - putative metal resistance protein (VIMSS), at 515,807 to 516,586
ybbM
b0492: ybbN - putative thioredoxin-like protein (VIMSS), at 516,649 to 517,503
ybbN
b0493: ybbO - short chain dehydrogenase (NCBI), at 517,564 to 518,373
ybbO
b0494: tesA - multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1 (NCBI), at 518,363 to 518,989
tesA
b0495: ybbA - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 518,957 to 519,643
ybbA
Position (kb) 517
518
519 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 516.634 kb on - strand at 516.636 kb on + strand at 516.636 kb on + strand at 516.644 kb on + strand at 516.667 kb on + strand at 516.667 kb on + strand at 516.670 kb on - strand at 516.707 kb on - strand at 516.824 kb on - strand, within ybbN at 516.922 kb on + strand, within ybbN at 516.972 kb on - strand, within ybbN at 516.972 kb on - strand, within ybbN at 516.986 kb on + strand, within ybbN at 517.018 kb on + strand, within ybbN at 517.026 kb on - strand, within ybbN at 517.026 kb on - strand, within ybbN at 517.096 kb on - strand, within ybbN at 517.096 kb on - strand, within ybbN at 517.181 kb on + strand, within ybbN at 517.255 kb on + strand, within ybbN at 517.308 kb on - strand, within ybbN at 517.308 kb on - strand, within ybbN at 517.340 kb on - strand, within ybbN at 517.345 kb on + strand, within ybbN at 517.401 kb on - strand, within ybbN at 517.433 kb on - strand at 517.444 kb on - strand at 517.857 kb on + strand, within ybbO at 517.902 kb on + strand, within ybbO at 517.902 kb on + strand, within ybbO at 517.957 kb on + strand, within ybbO at 518.037 kb on + strand, within ybbO at 518.051 kb on - strand, within ybbO at 518.123 kb on + strand, within ybbO at 518.145 kb on - strand, within ybbO at 518.145 kb on - strand, within ybbO at 518.199 kb on - strand, within ybbO at 518.377 kb on - strand at 518.475 kb on + strand, within tesA at 518.483 kb on - strand, within tesA at 518.570 kb on + strand, within tesA at 518.768 kb on + strand, within tesA at 518.839 kb on - strand, within tesA at 518.839 kb on - strand, within tesA at 518.839 kb on - strand, within tesA at 519.006 kb on + strand at 519.102 kb on + strand, within ybbA at 519.181 kb on - strand, within ybbA at 519.306 kb on - strand, within ybbA at 519.306 kb on - strand, within ybbA at 519.353 kb on + strand, within ybbA at 519.353 kb on + strand at 519.354 kb on + strand, within ybbA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM remove 516,634 - -1.3 516,636 + -0.2 516,636 + -0.2 516,644 + -0.2 516,667 + +0.8 516,667 + +0.7 516,670 - -1.7 516,707 - +1.0 516,824 - ybbN b0492 0.20 -1.5 516,922 + ybbN b0492 0.32 +1.9 516,972 - ybbN b0492 0.38 -0.7 516,972 - ybbN b0492 0.38 +0.3 516,986 + ybbN b0492 0.39 -0.4 517,018 + ybbN b0492 0.43 -0.3 517,026 - ybbN b0492 0.44 -1.5 517,026 - ybbN b0492 0.44 -0.8 517,096 - ybbN b0492 0.52 -1.1 517,096 - ybbN b0492 0.52 -0.1 517,181 + ybbN b0492 0.62 -0.9 517,255 + ybbN b0492 0.71 +0.9 517,308 - ybbN b0492 0.77 -0.2 517,308 - ybbN b0492 0.77 -0.3 517,340 - ybbN b0492 0.81 +0.4 517,345 + ybbN b0492 0.81 +1.0 517,401 - ybbN b0492 0.88 -1.4 517,433 - -0.2 517,444 - -1.1 517,857 + ybbO b0493 0.36 +0.8 517,902 + ybbO b0493 0.42 +0.8 517,902 + ybbO b0493 0.42 +0.8 517,957 + ybbO b0493 0.49 +1.0 518,037 + ybbO b0493 0.58 -0.6 518,051 - ybbO b0493 0.60 -0.2 518,123 + ybbO b0493 0.69 +1.4 518,145 - ybbO b0493 0.72 +1.0 518,145 - ybbO b0493 0.72 -0.1 518,199 - ybbO b0493 0.78 +0.7 518,377 - -0.7 518,475 + tesA b0494 0.18 -1.4 518,483 - tesA b0494 0.19 -0.2 518,570 + tesA b0494 0.33 +0.6 518,768 + tesA b0494 0.65 +0.7 518,839 - tesA b0494 0.76 -0.9 518,839 - tesA b0494 0.76 -1.1 518,839 - tesA b0494 0.76 -0.1 519,006 + +0.5 519,102 + ybbA b0495 0.21 -3.0 519,181 - ybbA b0495 0.33 +0.2 519,306 - ybbA b0495 0.51 +2.0 519,306 - ybbA b0495 0.51 +0.5 519,353 + ybbA b0495 0.58 -1.0 519,353 + +0.2 519,354 + ybbA b0495 0.58 -0.8
Or see this region's nucleotide sequence