Experiment: LB Anaerobic with Nalidixic 0.00000195313 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt torY and cutC are separated by 387 nucleotides cutC and yecM are separated by 13 nucleotides yecM and argS are separated by 215 nucleotides
b1873: torY - TMAO reductase III (TorYZ), cytochrome c-type subunit (NCBI), at 1,955,056 to 1,956,156
torY
b1874: cutC - copper homeostasis protein (NCBI), at 1,956,544 to 1,957,290
cutC
b1875: yecM - orf, hypothetical protein (VIMSS), at 1,957,304 to 1,957,870
yecM
b1876: argS - arginyl-tRNA synthetase (NCBI), at 1,958,086 to 1,959,819
argS
Position (kb)
1956
1957
1958 Strain fitness (log2 ratio)
-1
0
1
2 at 1955.580 kb on - strand, within torY at 1955.599 kb on + strand, within torY at 1955.607 kb on + strand, within torY at 1955.669 kb on - strand, within torY at 1955.669 kb on - strand, within torY at 1955.672 kb on + strand, within torY at 1955.686 kb on - strand, within torY at 1955.733 kb on + strand, within torY at 1955.765 kb on + strand, within torY at 1955.765 kb on + strand, within torY at 1955.774 kb on + strand, within torY at 1955.800 kb on + strand, within torY at 1955.820 kb on + strand, within torY at 1955.820 kb on + strand, within torY at 1955.844 kb on + strand, within torY at 1955.854 kb on + strand, within torY at 1955.854 kb on + strand, within torY at 1955.969 kb on - strand, within torY at 1955.974 kb on - strand, within torY at 1956.405 kb on - strand at 1956.407 kb on - strand at 1956.407 kb on - strand at 1956.416 kb on - strand at 1956.416 kb on - strand at 1956.417 kb on - strand at 1956.417 kb on - strand at 1956.417 kb on - strand at 1956.417 kb on - strand at 1956.417 kb on - strand at 1956.417 kb on - strand at 1956.417 kb on - strand at 1956.505 kb on - strand at 1956.561 kb on + strand at 1956.628 kb on + strand, within cutC at 1956.628 kb on + strand, within cutC at 1956.709 kb on + strand, within cutC at 1956.764 kb on - strand, within cutC at 1957.248 kb on + strand at 1957.416 kb on + strand, within yecM at 1957.473 kb on - strand, within yecM at 1957.588 kb on - strand, within yecM at 1957.677 kb on + strand, within yecM at 1957.760 kb on - strand, within yecM at 1957.810 kb on - strand, within yecM at 1957.810 kb on - strand, within yecM at 1958.016 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.00000195313 mM remove 1,955,580 - torY b1873 0.48 -0.5 1,955,599 + torY b1873 0.49 +0.8 1,955,607 + torY b1873 0.50 -0.3 1,955,669 - torY b1873 0.56 -1.1 1,955,669 - torY b1873 0.56 +0.7 1,955,672 + torY b1873 0.56 -0.5 1,955,686 - torY b1873 0.57 -0.1 1,955,733 + torY b1873 0.61 -0.0 1,955,765 + torY b1873 0.64 -0.1 1,955,765 + torY b1873 0.64 +0.2 1,955,774 + torY b1873 0.65 -0.1 1,955,800 + torY b1873 0.68 +1.5 1,955,820 + torY b1873 0.69 +0.7 1,955,820 + torY b1873 0.69 +2.2 1,955,844 + torY b1873 0.72 +0.5 1,955,854 + torY b1873 0.72 +1.5 1,955,854 + torY b1873 0.72 +1.5 1,955,969 - torY b1873 0.83 +1.5 1,955,974 - torY b1873 0.83 +1.5 1,956,405 - -0.2 1,956,407 - +0.9 1,956,407 - -0.1 1,956,416 - +0.0 1,956,416 - -1.1 1,956,417 - -0.4 1,956,417 - +0.3 1,956,417 - +1.5 1,956,417 - +0.2 1,956,417 - -0.5 1,956,417 - -1.3 1,956,417 - +0.5 1,956,505 - -1.3 1,956,561 + +1.5 1,956,628 + cutC b1874 0.11 -0.8 1,956,628 + cutC b1874 0.11 -1.1 1,956,709 + cutC b1874 0.22 +0.2 1,956,764 - cutC b1874 0.29 -1.2 1,957,248 + +1.3 1,957,416 + yecM b1875 0.20 -0.1 1,957,473 - yecM b1875 0.30 -0.8 1,957,588 - yecM b1875 0.50 +0.0 1,957,677 + yecM b1875 0.66 -1.3 1,957,760 - yecM b1875 0.80 +0.4 1,957,810 - yecM b1875 0.89 +1.9 1,957,810 - yecM b1875 0.89 +0.8 1,958,016 + +0.1
Or see this region's nucleotide sequence