Experiment: LB Anaerobic with Nalidixic 0.00000195313 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaB and alr are separated by 52 nucleotides alr and tyrB are separated by 252 nucleotides
b4052: dnaB - replicative DNA helicase (NCBI), at 4,262,337 to 4,263,752
dnaB
b4053: alr - alanine racemase (NCBI), at 4,263,805 to 4,264,884
alr
b4054: tyrB - tyrosine aminotransferase, tyrosine-repressible, PLP-dependent (NCBI), at 4,265,137 to 4,266,330
tyrB
Position (kb)
4263
4264
4265 Strain fitness (log2 ratio)
-2
-1
0
1 at 4263.790 kb on + strand at 4263.828 kb on - strand at 4263.828 kb on - strand at 4263.871 kb on - strand at 4263.919 kb on + strand, within alr at 4263.928 kb on - strand, within alr at 4264.017 kb on + strand, within alr at 4264.017 kb on + strand, within alr at 4264.102 kb on - strand, within alr at 4264.142 kb on - strand, within alr at 4264.146 kb on - strand, within alr at 4264.146 kb on - strand, within alr at 4264.220 kb on - strand, within alr at 4264.232 kb on + strand, within alr at 4264.240 kb on - strand, within alr at 4264.240 kb on - strand, within alr at 4264.240 kb on - strand, within alr at 4264.240 kb on - strand, within alr at 4264.266 kb on + strand, within alr at 4264.406 kb on - strand, within alr at 4264.406 kb on - strand, within alr at 4264.424 kb on + strand, within alr at 4264.462 kb on + strand, within alr at 4264.525 kb on - strand, within alr at 4264.525 kb on - strand, within alr at 4264.525 kb on - strand, within alr at 4264.525 kb on - strand, within alr at 4264.620 kb on + strand, within alr at 4264.620 kb on + strand, within alr at 4264.639 kb on - strand, within alr at 4264.658 kb on + strand, within alr at 4264.658 kb on + strand, within alr at 4264.661 kb on + strand, within alr at 4264.661 kb on + strand, within alr at 4264.661 kb on - strand, within alr at 4264.664 kb on - strand, within alr at 4264.722 kb on - strand, within alr at 4264.729 kb on + strand, within alr at 4264.806 kb on - strand at 4264.872 kb on + strand at 4264.872 kb on + strand at 4264.999 kb on + strand at 4265.008 kb on - strand at 4265.009 kb on + strand at 4265.009 kb on + strand at 4265.017 kb on - strand at 4265.035 kb on - strand at 4265.035 kb on - strand at 4265.063 kb on - strand at 4265.063 kb on - strand at 4265.081 kb on - strand at 4265.084 kb on + strand at 4265.097 kb on - strand at 4265.211 kb on + strand at 4265.211 kb on + strand at 4265.213 kb on - strand at 4265.244 kb on + strand at 4265.349 kb on + strand, within tyrB at 4265.357 kb on + strand, within tyrB at 4265.357 kb on + strand, within tyrB at 4265.514 kb on - strand, within tyrB at 4265.519 kb on + strand, within tyrB at 4265.519 kb on + strand, within tyrB at 4265.569 kb on - strand, within tyrB at 4265.623 kb on - strand, within tyrB at 4265.650 kb on + strand, within tyrB at 4265.650 kb on + strand, within tyrB at 4265.654 kb on + strand, within tyrB at 4265.662 kb on - strand, within tyrB at 4265.662 kb on - strand, within tyrB at 4265.672 kb on + strand, within tyrB at 4265.687 kb on + strand, within tyrB at 4265.723 kb on - strand, within tyrB at 4265.723 kb on - strand, within tyrB at 4265.842 kb on + strand, within tyrB at 4265.845 kb on - strand, within tyrB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.00000195313 mM remove 4,263,790 + +0.2 4,263,828 - -0.2 4,263,828 - -1.4 4,263,871 - -0.4 4,263,919 + alr b4053 0.11 -1.5 4,263,928 - alr b4053 0.11 -0.2 4,264,017 + alr b4053 0.20 -0.5 4,264,017 + alr b4053 0.20 -2.4 4,264,102 - alr b4053 0.28 +0.3 4,264,142 - alr b4053 0.31 -0.8 4,264,146 - alr b4053 0.32 -1.9 4,264,146 - alr b4053 0.32 -1.3 4,264,220 - alr b4053 0.38 -1.9 4,264,232 + alr b4053 0.40 -0.6 4,264,240 - alr b4053 0.40 -1.1 4,264,240 - alr b4053 0.40 -0.8 4,264,240 - alr b4053 0.40 -0.9 4,264,240 - alr b4053 0.40 -0.1 4,264,266 + alr b4053 0.43 -0.9 4,264,406 - alr b4053 0.56 -1.0 4,264,406 - alr b4053 0.56 -1.6 4,264,424 + alr b4053 0.57 -1.9 4,264,462 + alr b4053 0.61 -0.7 4,264,525 - alr b4053 0.67 -1.9 4,264,525 - alr b4053 0.67 -1.7 4,264,525 - alr b4053 0.67 -0.9 4,264,525 - alr b4053 0.67 -1.4 4,264,620 + alr b4053 0.75 -1.4 4,264,620 + alr b4053 0.75 -0.7 4,264,639 - alr b4053 0.77 -0.9 4,264,658 + alr b4053 0.79 -0.9 4,264,658 + alr b4053 0.79 -0.4 4,264,661 + alr b4053 0.79 +0.1 4,264,661 + alr b4053 0.79 -1.3 4,264,661 - alr b4053 0.79 -1.2 4,264,664 - alr b4053 0.80 -2.2 4,264,722 - alr b4053 0.85 -0.1 4,264,729 + alr b4053 0.86 -1.0 4,264,806 - -0.7 4,264,872 + +0.4 4,264,872 + -0.1 4,264,999 + +0.2 4,265,008 - +0.1 4,265,009 + +0.3 4,265,009 + -0.4 4,265,017 - -0.9 4,265,035 - -0.7 4,265,035 - -0.0 4,265,063 - +0.9 4,265,063 - -1.0 4,265,081 - -0.1 4,265,084 + +0.4 4,265,097 - +0.4 4,265,211 + -2.4 4,265,211 + +0.1 4,265,213 - -0.4 4,265,244 + +0.4 4,265,349 + tyrB b4054 0.18 +0.4 4,265,357 + tyrB b4054 0.18 -0.6 4,265,357 + tyrB b4054 0.18 -0.5 4,265,514 - tyrB b4054 0.32 +0.0 4,265,519 + tyrB b4054 0.32 -0.3 4,265,519 + tyrB b4054 0.32 +1.1 4,265,569 - tyrB b4054 0.36 +0.1 4,265,623 - tyrB b4054 0.41 +1.2 4,265,650 + tyrB b4054 0.43 +0.7 4,265,650 + tyrB b4054 0.43 +0.1 4,265,654 + tyrB b4054 0.43 -0.0 4,265,662 - tyrB b4054 0.44 +0.0 4,265,662 - tyrB b4054 0.44 -0.2 4,265,672 + tyrB b4054 0.45 -0.6 4,265,687 + tyrB b4054 0.46 -0.1 4,265,723 - tyrB b4054 0.49 -2.0 4,265,723 - tyrB b4054 0.49 +0.2 4,265,842 + tyrB b4054 0.59 -1.0 4,265,845 - tyrB b4054 0.59 -0.1
Or see this region's nucleotide sequence