Strain Fitness in Escherichia coli BW25113 around b2732

Experiment: LB Anaerobic with Nalidixic 0.00000195313 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfhlA and ygbA are separated by 36 nucleotidesygbA and mutS are separated by 286 nucleotides b2731: fhlA - DNA-binding transcriptional activator (NCBI), at 2,852,360 to 2,854,438 fhlA b2732: ygbA - hypothetical protein (NCBI), at 2,854,475 to 2,854,828 ygbA b2733: mutS - DNA mismatch repair protein (NCBI), at 2,855,115 to 2,857,676 mutS Position (kb) 2854 2855Strain fitness (log2 ratio) -1 0 1at 2853.518 kb on + strand, within fhlAat 2853.526 kb on - strand, within fhlAat 2853.526 kb on - strand, within fhlAat 2853.869 kb on - strand, within fhlAat 2854.040 kb on - strand, within fhlAat 2854.040 kb on - strand, within fhlAat 2854.115 kb on + strand, within fhlAat 2854.129 kb on - strand, within fhlAat 2854.131 kb on + strand, within fhlAat 2854.192 kb on - strand, within fhlAat 2854.260 kb on + strandat 2854.282 kb on - strandat 2854.529 kb on - strand, within ygbAat 2854.552 kb on + strand, within ygbAat 2854.586 kb on - strand, within ygbAat 2854.622 kb on - strand, within ygbAat 2854.652 kb on - strand, within ygbAat 2854.652 kb on - strand, within ygbAat 2854.984 kb on + strandat 2854.984 kb on + strandat 2855.167 kb on - strandat 2855.314 kb on + strandat 2855.314 kb on + strandat 2855.723 kb on - strand, within mutSat 2855.747 kb on + strand, within mutSat 2855.821 kb on - strand, within mutSat 2855.821 kb on - strand, within mutS

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.00000195313 mM
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2,853,518 + fhlA b2731 0.56 +0.7
2,853,526 - fhlA b2731 0.56 -0.0
2,853,526 - fhlA b2731 0.56 +0.7
2,853,869 - fhlA b2731 0.73 +0.2
2,854,040 - fhlA b2731 0.81 -0.3
2,854,040 - fhlA b2731 0.81 +0.0
2,854,115 + fhlA b2731 0.84 -0.3
2,854,129 - fhlA b2731 0.85 -0.7
2,854,131 + fhlA b2731 0.85 -0.5
2,854,192 - fhlA b2731 0.88 +0.3
2,854,260 + +0.0
2,854,282 - +0.4
2,854,529 - ygbA b2732 0.15 +0.2
2,854,552 + ygbA b2732 0.22 +0.4
2,854,586 - ygbA b2732 0.31 +0.7
2,854,622 - ygbA b2732 0.42 +0.1
2,854,652 - ygbA b2732 0.50 +0.7
2,854,652 - ygbA b2732 0.50 +0.0
2,854,984 + -0.2
2,854,984 + -0.9
2,855,167 - +1.7
2,855,314 + +0.2
2,855,314 + +1.4
2,855,723 - mutS b2733 0.24 -0.2
2,855,747 + mutS b2733 0.25 -0.0
2,855,821 - mutS b2733 0.28 +0.5
2,855,821 - mutS b2733 0.28 -0.1

Or see this region's nucleotide sequence