Experiment: LB Anaerobic with Nalidixic 0.00000195313 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lepA and rseC are separated by 197 nucleotides rseC and rseB overlap by 4 nucleotides rseB and rseA overlap by 1 nucleotides rseA and rpoE are separated by 32 nucleotides
b2569: lepA - GTP-binding protein LepA (NCBI), at 2,703,347 to 2,705,146
lepA
b2570: rseC - RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI), at 2,705,344 to 2,705,823
rseC
b2571: rseB - periplasmic negative regulator of sigmaE (NCBI), at 2,705,820 to 2,706,776
rseB
b2572: rseA - anti-sigma factor (NCBI), at 2,706,776 to 2,707,426
rseA
b2573: rpoE - RNA polymerase, sigma 24 (sigma E) factor (NCBI), at 2,707,459 to 2,708,034
rpoE
Position (kb)
2705
2706
2707 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2704.865 kb on - strand, within lepA at 2704.992 kb on + strand at 2705.007 kb on - strand at 2705.209 kb on - strand at 2705.227 kb on - strand at 2705.227 kb on - strand at 2705.249 kb on - strand at 2705.259 kb on - strand at 2705.268 kb on + strand at 2705.500 kb on + strand, within rseC at 2705.500 kb on + strand, within rseC at 2705.554 kb on - strand, within rseC at 2705.594 kb on + strand, within rseC at 2705.652 kb on + strand, within rseC at 2705.652 kb on + strand, within rseC at 2705.662 kb on + strand, within rseC at 2705.662 kb on + strand, within rseC at 2705.673 kb on + strand, within rseC at 2705.699 kb on - strand, within rseC at 2705.699 kb on - strand, within rseC at 2705.760 kb on + strand, within rseC at 2705.761 kb on - strand, within rseC at 2705.761 kb on - strand, within rseC at 2705.783 kb on - strand at 2705.783 kb on - strand at 2705.814 kb on - strand at 2705.915 kb on + strand at 2705.915 kb on + strand at 2705.923 kb on - strand, within rseB at 2705.987 kb on - strand, within rseB at 2706.013 kb on + strand, within rseB at 2706.190 kb on - strand, within rseB at 2706.190 kb on - strand, within rseB at 2706.211 kb on - strand, within rseB at 2706.307 kb on + strand, within rseB at 2706.315 kb on - strand, within rseB at 2706.315 kb on - strand, within rseB at 2706.322 kb on + strand, within rseB at 2706.397 kb on - strand, within rseB at 2706.399 kb on + strand, within rseB at 2706.524 kb on + strand, within rseB at 2706.524 kb on + strand, within rseB at 2706.576 kb on + strand, within rseB at 2706.601 kb on + strand, within rseB at 2706.601 kb on + strand, within rseB at 2706.654 kb on + strand, within rseB at 2706.696 kb on + strand at 2706.711 kb on - strand at 2706.711 kb on - strand at 2706.724 kb on - strand at 2706.738 kb on + strand at 2706.754 kb on - strand at 2706.754 kb on - strand at 2706.822 kb on - strand at 2706.822 kb on - strand at 2706.857 kb on - strand, within rseA at 2706.857 kb on - strand, within rseA at 2706.857 kb on - strand, within rseA at 2706.867 kb on - strand, within rseA at 2706.867 kb on - strand, within rseA at 2706.909 kb on - strand, within rseA at 2706.909 kb on - strand, within rseA at 2706.945 kb on - strand, within rseA at 2707.085 kb on - strand, within rseA at 2707.102 kb on - strand, within rseA at 2707.183 kb on + strand, within rseA at 2707.254 kb on + strand, within rseA at 2707.254 kb on + strand, within rseA at 2707.426 kb on - strand at 2707.436 kb on - strand at 2707.436 kb on - strand at 2707.437 kb on - strand at 2707.440 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.00000195313 mM remove 2,704,865 - lepA b2569 0.84 +1.6 2,704,992 + +1.6 2,705,007 - -0.7 2,705,209 - +0.3 2,705,227 - -0.0 2,705,227 - -0.1 2,705,249 - -0.4 2,705,259 - -0.7 2,705,268 + +0.1 2,705,500 + rseC b2570 0.33 +0.2 2,705,500 + rseC b2570 0.33 -0.4 2,705,554 - rseC b2570 0.44 +0.7 2,705,594 + rseC b2570 0.52 -0.5 2,705,652 + rseC b2570 0.64 +0.0 2,705,652 + rseC b2570 0.64 -1.2 2,705,662 + rseC b2570 0.66 -0.3 2,705,662 + rseC b2570 0.66 +0.6 2,705,673 + rseC b2570 0.69 -0.3 2,705,699 - rseC b2570 0.74 +1.3 2,705,699 - rseC b2570 0.74 +0.5 2,705,760 + rseC b2570 0.87 +0.1 2,705,761 - rseC b2570 0.87 +2.3 2,705,761 - rseC b2570 0.87 -2.0 2,705,783 - +3.6 2,705,783 - +1.9 2,705,814 - -0.3 2,705,915 + -1.0 2,705,915 + +0.2 2,705,923 - rseB b2571 0.11 -1.5 2,705,987 - rseB b2571 0.17 -0.9 2,706,013 + rseB b2571 0.20 -1.6 2,706,190 - rseB b2571 0.39 -0.3 2,706,190 - rseB b2571 0.39 -1.4 2,706,211 - rseB b2571 0.41 -1.1 2,706,307 + rseB b2571 0.51 -1.2 2,706,315 - rseB b2571 0.52 +0.4 2,706,315 - rseB b2571 0.52 +0.6 2,706,322 + rseB b2571 0.52 -0.9 2,706,397 - rseB b2571 0.60 -0.2 2,706,399 + rseB b2571 0.61 -1.2 2,706,524 + rseB b2571 0.74 +0.2 2,706,524 + rseB b2571 0.74 -2.0 2,706,576 + rseB b2571 0.79 -2.6 2,706,601 + rseB b2571 0.82 -1.3 2,706,601 + rseB b2571 0.82 -1.1 2,706,654 + rseB b2571 0.87 -1.6 2,706,696 + -0.8 2,706,711 - -1.5 2,706,711 - -1.5 2,706,724 - -1.7 2,706,738 + -1.6 2,706,754 - -1.0 2,706,754 - -0.7 2,706,822 - -1.1 2,706,822 - -0.1 2,706,857 - rseA b2572 0.12 -1.2 2,706,857 - rseA b2572 0.12 -0.5 2,706,857 - rseA b2572 0.12 -1.3 2,706,867 - rseA b2572 0.14 -1.5 2,706,867 - rseA b2572 0.14 -1.0 2,706,909 - rseA b2572 0.20 -0.3 2,706,909 - rseA b2572 0.20 -0.5 2,706,945 - rseA b2572 0.26 +0.0 2,707,085 - rseA b2572 0.47 -1.5 2,707,102 - rseA b2572 0.50 -0.5 2,707,183 + rseA b2572 0.63 +0.0 2,707,254 + rseA b2572 0.73 +0.0 2,707,254 + rseA b2572 0.73 -1.0 2,707,426 - +0.1 2,707,436 - -0.3 2,707,436 - -0.1 2,707,437 - -0.0 2,707,440 + -0.4
Or see this region's nucleotide sequence