Strain Fitness in Escherichia coli BW25113 around b2298

Experiment: LB Anaerobic with Nalidixic 0.00000195313 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpta and yfcC are separated by 189 nucleotidesyfcC and yfcD are separated by 32 nucleotidesyfcD and yfcE are separated by 57 nucleotides b2297: pta - phosphate acetyltransferase (NCBI), at 2,412,769 to 2,414,913 pta b2298: yfcC - putative S-transferase (VIMSS), at 2,415,103 to 2,416,623 yfcC b2299: yfcD - predicted NUDIX hydrolase (NCBI), at 2,416,656 to 2,417,198 yfcD b2300: yfcE - orf, hypothetical protein (VIMSS), at 2,417,256 to 2,417,810 yfcE Position (kb) 2415 2416 2417Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2414.132 kb on - strand, within ptaat 2415.137 kb on - strandat 2415.230 kb on + strandat 2415.230 kb on + strandat 2415.251 kb on - strandat 2415.285 kb on - strand, within yfcCat 2415.285 kb on - strand, within yfcCat 2415.345 kb on + strand, within yfcCat 2415.658 kb on + strand, within yfcCat 2415.679 kb on + strand, within yfcCat 2415.679 kb on + strand, within yfcCat 2415.692 kb on + strand, within yfcCat 2415.812 kb on - strand, within yfcCat 2415.835 kb on + strand, within yfcCat 2415.846 kb on + strand, within yfcCat 2415.847 kb on + strand, within yfcCat 2415.860 kb on - strand, within yfcCat 2415.860 kb on - strand, within yfcCat 2415.891 kb on + strand, within yfcCat 2415.962 kb on + strand, within yfcCat 2415.962 kb on + strand, within yfcCat 2415.979 kb on - strand, within yfcCat 2416.167 kb on - strand, within yfcCat 2416.240 kb on + strand, within yfcCat 2416.240 kb on + strand, within yfcCat 2416.315 kb on - strand, within yfcCat 2416.315 kb on - strand, within yfcCat 2416.355 kb on + strand, within yfcCat 2416.358 kb on - strand, within yfcCat 2416.358 kb on - strand, within yfcCat 2416.420 kb on - strand, within yfcCat 2416.456 kb on + strand, within yfcCat 2416.475 kb on + strandat 2416.502 kb on + strandat 2416.507 kb on + strandat 2416.512 kb on + strandat 2416.512 kb on + strandat 2416.516 kb on - strandat 2416.532 kb on - strandat 2416.532 kb on - strandat 2416.552 kb on + strandat 2416.605 kb on + strandat 2416.606 kb on - strandat 2416.609 kb on + strandat 2416.736 kb on + strand, within yfcDat 2416.811 kb on - strand, within yfcDat 2416.829 kb on + strand, within yfcDat 2416.973 kb on + strand, within yfcDat 2416.977 kb on - strand, within yfcDat 2416.977 kb on - strand, within yfcDat 2416.977 kb on - strand, within yfcDat 2417.064 kb on + strand, within yfcDat 2417.124 kb on + strand, within yfcDat 2417.124 kb on + strand, within yfcDat 2417.162 kb on - strandat 2417.177 kb on + strandat 2417.225 kb on + strandat 2417.235 kb on - strandat 2417.408 kb on + strand, within yfcE

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.00000195313 mM
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2,414,132 - pta b2297 0.64 +1.6
2,415,137 - +0.2
2,415,230 + +0.6
2,415,230 + -0.4
2,415,251 - +0.5
2,415,285 - yfcC b2298 0.12 +0.6
2,415,285 - yfcC b2298 0.12 +1.6
2,415,345 + yfcC b2298 0.16 -0.4
2,415,658 + yfcC b2298 0.36 +0.4
2,415,679 + yfcC b2298 0.38 -0.9
2,415,679 + yfcC b2298 0.38 -0.6
2,415,692 + yfcC b2298 0.39 -1.6
2,415,812 - yfcC b2298 0.47 +0.7
2,415,835 + yfcC b2298 0.48 +0.9
2,415,846 + yfcC b2298 0.49 +0.2
2,415,847 + yfcC b2298 0.49 +0.2
2,415,860 - yfcC b2298 0.50 +1.9
2,415,860 - yfcC b2298 0.50 +0.5
2,415,891 + yfcC b2298 0.52 -0.0
2,415,962 + yfcC b2298 0.56 -1.4
2,415,962 + yfcC b2298 0.56 -0.0
2,415,979 - yfcC b2298 0.58 +1.0
2,416,167 - yfcC b2298 0.70 +0.3
2,416,240 + yfcC b2298 0.75 -0.3
2,416,240 + yfcC b2298 0.75 -2.8
2,416,315 - yfcC b2298 0.80 +0.2
2,416,315 - yfcC b2298 0.80 -0.6
2,416,355 + yfcC b2298 0.82 +0.3
2,416,358 - yfcC b2298 0.83 +2.0
2,416,358 - yfcC b2298 0.83 +1.2
2,416,420 - yfcC b2298 0.87 +1.0
2,416,456 + yfcC b2298 0.89 -0.3
2,416,475 + +0.8
2,416,502 + -1.5
2,416,507 + +0.6
2,416,512 + -1.3
2,416,512 + -0.1
2,416,516 - -0.3
2,416,532 - -0.1
2,416,532 - +1.1
2,416,552 + +0.2
2,416,605 + -0.1
2,416,606 - +0.3
2,416,609 + +0.9
2,416,736 + yfcD b2299 0.15 -1.7
2,416,811 - yfcD b2299 0.29 -0.5
2,416,829 + yfcD b2299 0.32 -0.7
2,416,973 + yfcD b2299 0.58 +0.4
2,416,977 - yfcD b2299 0.59 +1.6
2,416,977 - yfcD b2299 0.59 +1.4
2,416,977 - yfcD b2299 0.59 -0.0
2,417,064 + yfcD b2299 0.75 +0.1
2,417,124 + yfcD b2299 0.86 +2.0
2,417,124 + yfcD b2299 0.86 +0.1
2,417,162 - -0.4
2,417,177 + -0.8
2,417,225 + -0.4
2,417,235 - -0.6
2,417,408 + yfcE b2300 0.27 -1.7

Or see this region's nucleotide sequence