Strain Fitness in Escherichia coli BW25113 around b2009

Experiment: LB Anaerobic with Nalidixic 0.00000195313 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyeeA and sbmC are separated by 197 nucleotidessbmC and dacD are separated by 118 nucleotides b2008: yeeA - conserved inner membrane protein (NCBI), at 2,077,557 to 2,078,615 yeeA b2009: sbmC - DNA gyrase inhibitor (NCBI), at 2,078,813 to 2,079,286 sbmC b2010: dacD - penicillin binding protein 6b (VIMSS), at 2,079,405 to 2,080,571 dacD Position (kb) 2078 2079 2080Strain fitness (log2 ratio) -1 0 1at 2077.858 kb on - strand, within yeeAat 2077.895 kb on + strand, within yeeAat 2077.895 kb on + strand, within yeeAat 2078.047 kb on - strand, within yeeAat 2078.068 kb on + strand, within yeeAat 2078.115 kb on - strand, within yeeAat 2078.611 kb on - strandat 2078.684 kb on - strandat 2078.689 kb on - strandat 2078.853 kb on + strandat 2078.861 kb on - strand, within sbmCat 2078.864 kb on + strand, within sbmCat 2079.000 kb on + strand, within sbmCat 2079.077 kb on + strand, within sbmCat 2079.281 kb on + strandat 2079.472 kb on - strandat 2079.472 kb on - strandat 2079.505 kb on - strandat 2079.568 kb on - strand, within dacDat 2079.612 kb on + strand, within dacDat 2079.630 kb on + strand, within dacDat 2079.784 kb on + strand, within dacDat 2079.833 kb on - strand, within dacDat 2079.833 kb on - strand, within dacDat 2079.857 kb on + strand, within dacDat 2079.857 kb on + strand, within dacDat 2079.966 kb on + strand, within dacDat 2080.001 kb on + strand, within dacDat 2080.041 kb on + strand, within dacD

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.00000195313 mM
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2,077,858 - yeeA b2008 0.28 +0.4
2,077,895 + yeeA b2008 0.32 +0.5
2,077,895 + yeeA b2008 0.32 +0.5
2,078,047 - yeeA b2008 0.46 +0.7
2,078,068 + yeeA b2008 0.48 -0.0
2,078,115 - yeeA b2008 0.53 -0.4
2,078,611 - -0.7
2,078,684 - -0.5
2,078,689 - +0.6
2,078,853 + -0.5
2,078,861 - sbmC b2009 0.10 +0.2
2,078,864 + sbmC b2009 0.11 +0.8
2,079,000 + sbmC b2009 0.39 +0.1
2,079,077 + sbmC b2009 0.56 +0.5
2,079,281 + +1.5
2,079,472 - +1.0
2,079,472 - +0.5
2,079,505 - -0.9
2,079,568 - dacD b2010 0.14 -0.2
2,079,612 + dacD b2010 0.18 -1.3
2,079,630 + dacD b2010 0.19 -0.1
2,079,784 + dacD b2010 0.32 -0.2
2,079,833 - dacD b2010 0.37 -0.2
2,079,833 - dacD b2010 0.37 +0.5
2,079,857 + dacD b2010 0.39 +1.0
2,079,857 + dacD b2010 0.39 -0.5
2,079,966 + dacD b2010 0.48 -0.8
2,080,001 + dacD b2010 0.51 -0.1
2,080,041 + dacD b2010 0.54 +1.5

Or see this region's nucleotide sequence