Strain Fitness in Escherichia coli BW25113 around b1475

Experiment: LB Anaerobic with Nalidixic 0.00000195313 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfdnG and fdnH are separated by 12 nucleotidesfdnH and fdnI overlap by 8 nucleotides b1474: fdnG - formate dehydrogenase-N, alpha subunit, nitrate-inducible (NCBI), at 1,545,425 to 1,548,472 fdnG b1475: fdnH - formate dehydrogenase-N, Fe-S (beta) subunit, nitrate-inducible (NCBI), at 1,548,485 to 1,549,369 fdnH b1476: fdnI - formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI), at 1,549,362 to 1,550,015 fdnI Position (kb) 1548 1549 1550Strain fitness (log2 ratio) -1 0 1at 1547.748 kb on + strand, within fdnGat 1547.748 kb on + strand, within fdnGat 1548.012 kb on + strand, within fdnGat 1548.097 kb on + strand, within fdnGat 1548.097 kb on + strand, within fdnGat 1548.098 kb on + strand, within fdnGat 1548.444 kb on - strandat 1548.444 kb on - strandat 1548.465 kb on + strandat 1548.607 kb on - strand, within fdnHat 1548.745 kb on - strand, within fdnHat 1548.745 kb on - strand, within fdnHat 1548.789 kb on + strand, within fdnHat 1548.789 kb on + strand, within fdnHat 1549.042 kb on - strand, within fdnHat 1549.104 kb on + strand, within fdnHat 1549.104 kb on + strand, within fdnHat 1549.200 kb on - strand, within fdnHat 1549.396 kb on - strandat 1549.396 kb on - strandat 1549.813 kb on - strand, within fdnIat 1550.056 kb on - strandat 1550.189 kb on - strandat 1550.189 kb on - strandat 1550.209 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.00000195313 mM
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1,547,748 + fdnG b1474 0.76 -0.4
1,547,748 + fdnG b1474 0.76 +0.2
1,548,012 + fdnG b1474 0.85 +0.1
1,548,097 + fdnG b1474 0.88 -0.8
1,548,097 + fdnG b1474 0.88 +0.3
1,548,098 + fdnG b1474 0.88 -0.2
1,548,444 - +0.5
1,548,444 - +0.1
1,548,465 + +0.5
1,548,607 - fdnH b1475 0.14 +0.2
1,548,745 - fdnH b1475 0.29 -0.2
1,548,745 - fdnH b1475 0.29 +0.0
1,548,789 + fdnH b1475 0.34 +0.2
1,548,789 + fdnH b1475 0.34 +0.3
1,549,042 - fdnH b1475 0.63 +0.9
1,549,104 + fdnH b1475 0.70 +0.0
1,549,104 + fdnH b1475 0.70 +1.2
1,549,200 - fdnH b1475 0.81 +0.8
1,549,396 - +1.5
1,549,396 - -0.7
1,549,813 - fdnI b1476 0.69 -1.2
1,550,056 - -0.9
1,550,189 - -0.5
1,550,189 - -0.9
1,550,209 - -0.4

Or see this region's nucleotide sequence