Experiment: LB Anaerobic with Nalidixic 0.00000195313 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt paaF and paaG overlap by 1 nucleotides paaG and paaH are separated by 1 nucleotides paaH and paaI overlap by 11 nucleotides paaI and paaJ overlap by 1 nucleotides
b1393: paaF - enoyl-CoA hydratase-isomerase (NCBI), at 1,455,521 to 1,456,288
paaF
b1394: paaG - enoyl-CoA hydratase (NCBI), at 1,456,288 to 1,457,076
paaG
b1395: paaH - 3-hydroxybutyryl-CoA dehydrogenase (NCBI), at 1,457,078 to 1,458,505
paaH
b1396: paaI - predicted thioesterase (NCBI), at 1,458,495 to 1,458,917
paaI
b1397: paaJ - acetyl-CoA acetyltransferase (NCBI), at 1,458,917 to 1,460,122
paaJ
Position (kb)
1457
1458
1459 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1456.216 kb on - strand at 1456.216 kb on - strand at 1456.490 kb on + strand, within paaG at 1456.637 kb on + strand, within paaG at 1456.780 kb on - strand, within paaG at 1456.910 kb on + strand, within paaG at 1457.029 kb on + strand at 1457.029 kb on + strand at 1457.192 kb on + strand at 1457.264 kb on + strand, within paaH at 1457.625 kb on + strand, within paaH at 1457.625 kb on + strand, within paaH at 1457.668 kb on + strand, within paaH at 1457.750 kb on + strand, within paaH at 1457.822 kb on + strand, within paaH at 1457.921 kb on - strand, within paaH at 1457.921 kb on - strand, within paaH at 1457.941 kb on + strand, within paaH at 1457.979 kb on + strand, within paaH at 1458.061 kb on - strand, within paaH at 1458.061 kb on - strand, within paaH at 1458.104 kb on - strand, within paaH at 1458.104 kb on - strand, within paaH at 1458.138 kb on + strand, within paaH at 1458.207 kb on + strand, within paaH at 1458.487 kb on - strand at 1458.499 kb on + strand at 1458.558 kb on - strand, within paaI at 1458.590 kb on - strand, within paaI at 1458.590 kb on - strand, within paaI at 1458.602 kb on + strand, within paaI at 1458.602 kb on + strand, within paaI at 1458.679 kb on - strand, within paaI at 1458.689 kb on - strand, within paaI at 1458.786 kb on + strand, within paaI at 1458.909 kb on - strand at 1458.909 kb on - strand at 1458.914 kb on - strand at 1458.914 kb on - strand at 1459.088 kb on + strand, within paaJ at 1459.188 kb on + strand, within paaJ at 1459.188 kb on + strand, within paaJ at 1459.425 kb on + strand, within paaJ at 1459.425 kb on + strand, within paaJ
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.00000195313 mM remove 1,456,216 - +2.2 1,456,216 - +1.0 1,456,490 + paaG b1394 0.26 -2.1 1,456,637 + paaG b1394 0.44 -1.1 1,456,780 - paaG b1394 0.62 +0.6 1,456,910 + paaG b1394 0.79 +0.5 1,457,029 + +0.8 1,457,029 + +0.6 1,457,192 + +0.6 1,457,264 + paaH b1395 0.13 +0.5 1,457,625 + paaH b1395 0.38 -0.3 1,457,625 + paaH b1395 0.38 -0.6 1,457,668 + paaH b1395 0.41 -1.9 1,457,750 + paaH b1395 0.47 -1.3 1,457,822 + paaH b1395 0.52 -1.1 1,457,921 - paaH b1395 0.59 -1.4 1,457,921 - paaH b1395 0.59 -1.1 1,457,941 + paaH b1395 0.60 -1.7 1,457,979 + paaH b1395 0.63 -0.3 1,458,061 - paaH b1395 0.69 +1.0 1,458,061 - paaH b1395 0.69 +1.5 1,458,104 - paaH b1395 0.72 -0.1 1,458,104 - paaH b1395 0.72 -0.9 1,458,138 + paaH b1395 0.74 -0.9 1,458,207 + paaH b1395 0.79 -1.0 1,458,487 - -0.4 1,458,499 + +0.0 1,458,558 - paaI b1396 0.15 +1.5 1,458,590 - paaI b1396 0.22 -0.5 1,458,590 - paaI b1396 0.22 -0.8 1,458,602 + paaI b1396 0.25 -0.1 1,458,602 + paaI b1396 0.25 -0.4 1,458,679 - paaI b1396 0.43 +0.0 1,458,689 - paaI b1396 0.46 -0.1 1,458,786 + paaI b1396 0.69 -1.0 1,458,909 - +0.3 1,458,909 - -1.1 1,458,914 - +0.9 1,458,914 - +0.5 1,459,088 + paaJ b1397 0.14 +0.1 1,459,188 + paaJ b1397 0.22 +0.1 1,459,188 + paaJ b1397 0.22 +0.6 1,459,425 + paaJ b1397 0.42 -2.5 1,459,425 + paaJ b1397 0.42 -0.1
Or see this region's nucleotide sequence