Experiment: LB Anaerobic with Nalidixic 0.00000195313 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycjD and sapF are separated by 67 nucleotides sapF and sapD are separated by 1 nucleotides sapD and sapC overlap by 1 nucleotides sapC and sapB overlap by 14 nucleotides
b1289: ycjD - hypothetical protein (NCBI), at 1,349,431 to 1,349,784
ycjD
b1290: sapF - predicted antimicrobial peptide transporter subunit (NCBI), at 1,349,852 to 1,350,658
sapF
b1291: sapD - predicted antimicrobial peptide transporter subunit (NCBI), at 1,350,660 to 1,351,652
sapD
b1292: sapC - predicted antimicrobial peptide transporter subunit (NCBI), at 1,351,652 to 1,352,542
sapC
b1293: sapB - predicted antimicrobial peptide transporter subunit (NCBI), at 1,352,529 to 1,353,494
sapB
Position (kb)
1350
1351
1352 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1349.963 kb on - strand, within sapF at 1350.108 kb on + strand, within sapF at 1350.244 kb on + strand, within sapF at 1350.286 kb on + strand, within sapF at 1350.294 kb on - strand, within sapF at 1350.550 kb on + strand, within sapF at 1350.656 kb on - strand at 1350.749 kb on + strand at 1350.855 kb on + strand, within sapD at 1350.861 kb on + strand, within sapD at 1350.997 kb on - strand, within sapD at 1351.021 kb on - strand, within sapD at 1351.342 kb on - strand, within sapD at 1351.739 kb on - strand at 1352.074 kb on - strand, within sapC at 1352.113 kb on + strand, within sapC at 1352.173 kb on + strand, within sapC at 1352.173 kb on + strand, within sapC at 1352.254 kb on + strand, within sapC at 1352.290 kb on - strand, within sapC at 1352.351 kb on - strand, within sapC at 1352.438 kb on + strand, within sapC
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.00000195313 mM remove 1,349,963 - sapF b1290 0.14 -0.1 1,350,108 + sapF b1290 0.32 +0.5 1,350,244 + sapF b1290 0.49 +0.9 1,350,286 + sapF b1290 0.54 -0.1 1,350,294 - sapF b1290 0.55 +0.2 1,350,550 + sapF b1290 0.86 +1.5 1,350,656 - +0.3 1,350,749 + +0.9 1,350,855 + sapD b1291 0.20 -0.1 1,350,861 + sapD b1291 0.20 +0.5 1,350,997 - sapD b1291 0.34 -1.7 1,351,021 - sapD b1291 0.36 +2.8 1,351,342 - sapD b1291 0.69 -0.5 1,351,739 - +0.1 1,352,074 - sapC b1292 0.47 +1.5 1,352,113 + sapC b1292 0.52 +0.7 1,352,173 + sapC b1292 0.58 -0.5 1,352,173 + sapC b1292 0.58 +1.5 1,352,254 + sapC b1292 0.68 -0.5 1,352,290 - sapC b1292 0.72 +2.7 1,352,351 - sapC b1292 0.78 +2.1 1,352,438 + sapC b1292 0.88 +0.5
Or see this region's nucleotide sequence