Strain Fitness in Escherichia coli BW25113 around b1219

Experiment: LB Anaerobic with Nalidixic 0.00000195313 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntchaB and chaC are separated by 157 nucleotideschaC and ychN are separated by 43 nucleotidesychN and ychO are separated by 184 nucleotides b1217: chaB - cation transport regulator (NCBI), at 1,271,342 to 1,271,572 chaB b1218: chaC - regulatory protein for cation transport (RefSeq), at 1,271,730 to 1,272,425 chaC b1219: ychN - hypothetical protein (NCBI), at 1,272,469 to 1,272,822 ychN b1220: ychO - predicted invasin (RefSeq), at 1,273,007 to 1,274,401 ychO Position (kb) 1272 1273Strain fitness (log2 ratio) -1 0 1at 1271.475 kb on - strand, within chaBat 1271.723 kb on + strandat 1271.892 kb on + strand, within chaCat 1271.892 kb on + strand, within chaCat 1271.932 kb on - strand, within chaCat 1272.143 kb on - strand, within chaCat 1272.159 kb on - strand, within chaCat 1272.288 kb on - strand, within chaCat 1272.522 kb on - strand, within ychNat 1272.532 kb on - strand, within ychNat 1272.581 kb on - strand, within ychNat 1272.615 kb on - strand, within ychNat 1272.714 kb on + strand, within ychNat 1272.787 kb on - strandat 1272.787 kb on - strandat 1273.039 kb on + strandat 1273.305 kb on + strand, within ychOat 1273.328 kb on - strand, within ychOat 1273.328 kb on - strand, within ychOat 1273.463 kb on + strand, within ychOat 1273.493 kb on + strand, within ychOat 1273.732 kb on + strand, within ychOat 1273.737 kb on + strand, within ychO

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.00000195313 mM
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1,271,475 - chaB b1217 0.58 +1.2
1,271,723 + +0.2
1,271,892 + chaC b1218 0.23 +0.4
1,271,892 + chaC b1218 0.23 +0.4
1,271,932 - chaC b1218 0.29 +1.1
1,272,143 - chaC b1218 0.59 -1.0
1,272,159 - chaC b1218 0.62 +1.2
1,272,288 - chaC b1218 0.80 -0.5
1,272,522 - ychN b1219 0.15 +0.1
1,272,532 - ychN b1219 0.18 +0.9
1,272,581 - ychN b1219 0.32 +0.6
1,272,615 - ychN b1219 0.41 -0.1
1,272,714 + ychN b1219 0.69 -0.5
1,272,787 - +1.1
1,272,787 - -1.0
1,273,039 + -0.1
1,273,305 + ychO b1220 0.21 +0.1
1,273,328 - ychO b1220 0.23 +0.1
1,273,328 - ychO b1220 0.23 -0.0
1,273,463 + ychO b1220 0.33 +0.4
1,273,493 + ychO b1220 0.35 +0.4
1,273,732 + ychO b1220 0.52 -0.5
1,273,737 + ychO b1220 0.52 -0.0

Or see this region's nucleotide sequence