Experiment: LB Anaerobic with Nalidixic 0.00000195313 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt macB and cspD are separated by 72 nucleotides cspD and clpS are separated by 322 nucleotides clpS and clpA are separated by 30 nucleotides
b0879: macB - fused macrolide transporter subunits of ABC superfamily: ATP-binding component/membrane component (NCBI), at 919,570 to 921,516
macB
b0880: cspD - cold shock protein homolog (NCBI), at 921,589 to 921,813
cspD
b0881: clpS - ATP-dependent Clp protease adaptor protein ClpS (NCBI), at 922,136 to 922,456
clpS
b0882: clpA - ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI), at 922,487 to 924,763
clpA
Position (kb) 922
923 Strain fitness (log2 ratio) -2
-1
0
1 at 921.164 kb on + strand, within macB at 921.169 kb on + strand, within macB at 921.254 kb on - strand, within macB at 921.254 kb on - strand, within macB at 921.271 kb on + strand, within macB at 921.298 kb on + strand, within macB at 921.298 kb on + strand, within macB at 921.790 kb on + strand, within cspD at 921.790 kb on + strand, within cspD at 921.956 kb on - strand at 921.956 kb on - strand at 922.042 kb on + strand at 922.042 kb on + strand at 922.055 kb on + strand at 922.069 kb on + strand at 922.091 kb on - strand at 922.103 kb on + strand at 922.124 kb on + strand at 922.246 kb on - strand, within clpS at 922.417 kb on + strand, within clpS at 922.435 kb on - strand at 922.562 kb on + strand at 922.686 kb on - strand at 922.695 kb on - strand at 922.703 kb on + strand at 922.703 kb on + strand at 922.745 kb on + strand, within clpA at 922.745 kb on + strand, within clpA at 922.767 kb on + strand, within clpA at 922.767 kb on + strand, within clpA at 922.850 kb on + strand, within clpA at 922.877 kb on - strand, within clpA at 922.904 kb on - strand, within clpA at 923.228 kb on + strand, within clpA at 923.236 kb on - strand, within clpA at 923.381 kb on + strand, within clpA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.00000195313 mM remove 921,164 + macB b0879 0.82 +0.2 921,169 + macB b0879 0.82 -0.1 921,254 - macB b0879 0.86 -1.1 921,254 - macB b0879 0.86 +0.5 921,271 + macB b0879 0.87 +0.1 921,298 + macB b0879 0.89 -0.5 921,298 + macB b0879 0.89 -0.1 921,790 + cspD b0880 0.89 +0.1 921,790 + cspD b0880 0.89 -0.2 921,956 - +0.1 921,956 - +0.5 922,042 + -1.2 922,042 + -0.1 922,055 + +0.1 922,069 + +0.4 922,091 - +0.5 922,103 + -0.3 922,124 + -0.3 922,246 - clpS b0881 0.34 -1.4 922,417 + clpS b0881 0.88 -1.8 922,435 - +0.9 922,562 + -0.3 922,686 - +1.2 922,695 - -0.6 922,703 + +0.3 922,703 + +0.9 922,745 + clpA b0882 0.11 +0.4 922,745 + clpA b0882 0.11 -0.3 922,767 + clpA b0882 0.12 -1.1 922,767 + clpA b0882 0.12 -0.4 922,850 + clpA b0882 0.16 +0.1 922,877 - clpA b0882 0.17 -1.3 922,904 - clpA b0882 0.18 -0.4 923,228 + clpA b0882 0.33 +0.3 923,236 - clpA b0882 0.33 +0.5 923,381 + clpA b0882 0.39 -0.4
Or see this region's nucleotide sequence