Experiment: LB Anaerobic with Nalidixic 0.00000195313 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mipB and moeB are separated by 75 nucleotides moeB and moeA overlap by 1 nucleotides moeA and iaaA are separated by 203 nucleotides
b0825: mipB - putative transaldolase (VIMSS), at 862,865 to 863,527
mipB
b0826: moeB - molybdopterin biosynthesis protein MoeB (NCBI), at 863,603 to 864,352
moeB
b0827: moeA - molybdopterin biosynthesis protein (NCBI), at 864,352 to 865,587
moeA
b0828: iaaA - L-asparaginase (NCBI), at 865,791 to 866,756
iaaA
Position (kb)
864
865
866 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 863.433 kb on + strand, within mipB at 863.554 kb on + strand at 863.625 kb on - strand at 863.673 kb on - strand at 863.881 kb on + strand, within moeB at 863.881 kb on + strand, within moeB at 863.980 kb on - strand, within moeB at 863.987 kb on + strand, within moeB at 864.031 kb on - strand, within moeB at 864.031 kb on - strand, within moeB at 864.040 kb on - strand, within moeB at 864.067 kb on + strand, within moeB at 864.071 kb on - strand, within moeB at 864.129 kb on + strand, within moeB at 864.167 kb on + strand, within moeB at 864.209 kb on - strand, within moeB at 864.209 kb on - strand, within moeB at 864.214 kb on - strand, within moeB at 864.271 kb on - strand, within moeB at 864.647 kb on + strand, within moeA at 864.770 kb on - strand, within moeA at 864.770 kb on - strand, within moeA at 864.814 kb on - strand, within moeA at 864.855 kb on + strand, within moeA at 864.945 kb on + strand, within moeA at 865.047 kb on + strand, within moeA at 865.064 kb on - strand, within moeA at 865.220 kb on + strand, within moeA at 865.243 kb on + strand, within moeA at 865.243 kb on + strand, within moeA at 865.278 kb on - strand, within moeA at 865.278 kb on - strand at 865.301 kb on - strand, within moeA at 865.527 kb on + strand at 865.527 kb on + strand at 865.535 kb on - strand at 865.561 kb on - strand at 865.646 kb on + strand at 865.646 kb on + strand at 865.648 kb on + strand at 865.771 kb on + strand at 865.771 kb on + strand at 865.875 kb on + strand at 865.883 kb on + strand at 866.064 kb on - strand, within iaaA at 866.149 kb on + strand, within iaaA at 866.246 kb on + strand at 866.424 kb on + strand, within iaaA at 866.432 kb on - strand, within iaaA at 866.537 kb on + strand, within iaaA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.00000195313 mM remove 863,433 + mipB b0825 0.86 +0.3 863,554 + +0.1 863,625 - -1.1 863,673 - -0.7 863,881 + moeB b0826 0.37 -0.9 863,881 + moeB b0826 0.37 -2.2 863,980 - moeB b0826 0.50 -1.4 863,987 + moeB b0826 0.51 -1.2 864,031 - moeB b0826 0.57 -1.0 864,031 - moeB b0826 0.57 -1.5 864,040 - moeB b0826 0.58 -0.5 864,067 + moeB b0826 0.62 +0.3 864,071 - moeB b0826 0.62 -0.2 864,129 + moeB b0826 0.70 -2.0 864,167 + moeB b0826 0.75 -0.8 864,209 - moeB b0826 0.81 -0.5 864,209 - moeB b0826 0.81 +0.1 864,214 - moeB b0826 0.81 -1.1 864,271 - moeB b0826 0.89 -1.3 864,647 + moeA b0827 0.24 -0.6 864,770 - moeA b0827 0.34 -3.1 864,770 - moeA b0827 0.34 -0.2 864,814 - moeA b0827 0.37 -0.6 864,855 + moeA b0827 0.41 -1.1 864,945 + moeA b0827 0.48 -1.3 865,047 + moeA b0827 0.56 -0.8 865,064 - moeA b0827 0.58 -0.9 865,220 + moeA b0827 0.70 +1.6 865,243 + moeA b0827 0.72 -2.5 865,243 + moeA b0827 0.72 -1.0 865,278 - moeA b0827 0.75 -0.9 865,278 - +1.6 865,301 - moeA b0827 0.77 -1.2 865,527 + +0.2 865,527 + +0.6 865,535 - -1.8 865,561 - -1.9 865,646 + -0.8 865,646 + +0.4 865,648 + +0.2 865,771 + -0.2 865,771 + -0.9 865,875 + -0.5 865,883 + +0.1 866,064 - iaaA b0828 0.28 +1.1 866,149 + iaaA b0828 0.37 -0.6 866,246 + +2.6 866,424 + iaaA b0828 0.66 -0.1 866,432 - iaaA b0828 0.66 +0.2 866,537 + iaaA b0828 0.77 -0.2
Or see this region's nucleotide sequence