Experiment: LB Anaerobic with Nalidixic 0.00000195313 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt acrB and acrA are separated by 22 nucleotides acrA and acrR are separated by 141 nucleotides acrR and kefA are separated by 127 nucleotides
b0462: acrB - multidrug efflux system protein (NCBI), at 480,478 to 483,627
acrB
b0463: acrA - multidrug efflux system (NCBI), at 483,650 to 484,843
acrA
b0464: acrR - DNA-binding transcriptional repressor (NCBI), at 484,985 to 485,632
acrR
b0465: kefA - fused conserved protein (NCBI), at 485,760 to 489,122
kefA
Position (kb) 483
484
485 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 482.650 kb on - strand, within acrB at 482.801 kb on + strand, within acrB at 482.823 kb on - strand, within acrB at 482.823 kb on - strand, within acrB at 482.870 kb on + strand, within acrB at 482.905 kb on + strand at 482.905 kb on + strand, within acrB at 482.932 kb on - strand, within acrB at 482.935 kb on + strand, within acrB at 482.935 kb on + strand, within acrB at 482.941 kb on - strand, within acrB at 482.969 kb on - strand, within acrB at 482.969 kb on - strand, within acrB at 482.978 kb on + strand, within acrB at 483.037 kb on - strand, within acrB at 483.037 kb on - strand, within acrB at 483.128 kb on - strand, within acrB at 483.128 kb on - strand, within acrB at 483.133 kb on - strand, within acrB at 483.301 kb on + strand, within acrB at 483.308 kb on + strand, within acrB at 483.309 kb on - strand, within acrB at 483.309 kb on - strand, within acrB at 483.316 kb on - strand at 483.333 kb on - strand at 483.358 kb on + strand at 483.366 kb on - strand at 483.376 kb on - strand at 483.383 kb on + strand at 483.383 kb on + strand at 483.383 kb on - strand at 483.393 kb on - strand at 483.439 kb on - strand at 483.451 kb on + strand at 483.451 kb on + strand at 483.515 kb on + strand at 483.540 kb on + strand at 483.584 kb on - strand at 483.622 kb on + strand at 483.635 kb on - strand at 483.636 kb on - strand at 483.636 kb on - strand at 483.638 kb on - strand at 483.672 kb on - strand at 483.789 kb on + strand, within acrA at 483.813 kb on + strand, within acrA at 483.813 kb on + strand, within acrA at 483.852 kb on - strand, within acrA at 483.852 kb on - strand, within acrA at 483.902 kb on + strand, within acrA at 483.903 kb on - strand, within acrA at 483.903 kb on - strand, within acrA at 483.903 kb on - strand, within acrA at 483.977 kb on + strand, within acrA at 483.987 kb on + strand, within acrA at 484.011 kb on - strand, within acrA at 484.027 kb on + strand, within acrA at 484.028 kb on - strand, within acrA at 484.028 kb on - strand, within acrA at 484.070 kb on + strand, within acrA at 484.073 kb on + strand, within acrA at 484.190 kb on - strand, within acrA at 484.202 kb on + strand, within acrA at 484.248 kb on + strand, within acrA at 484.251 kb on - strand, within acrA at 484.254 kb on + strand, within acrA at 484.261 kb on - strand, within acrA at 484.400 kb on + strand, within acrA at 484.453 kb on + strand, within acrA at 484.526 kb on + strand, within acrA at 484.526 kb on + strand, within acrA at 484.526 kb on + strand, within acrA at 484.526 kb on + strand, within acrA at 484.618 kb on - strand, within acrA at 484.618 kb on - strand, within acrA at 484.659 kb on + strand, within acrA at 484.662 kb on - strand, within acrA at 484.917 kb on - strand at 484.917 kb on - strand at 485.062 kb on + strand, within acrR at 485.071 kb on + strand, within acrR at 485.071 kb on + strand, within acrR at 485.145 kb on - strand, within acrR at 485.175 kb on - strand, within acrR at 485.193 kb on - strand, within acrR at 485.193 kb on - strand, within acrR at 485.199 kb on + strand, within acrR at 485.199 kb on + strand, within acrR at 485.239 kb on + strand, within acrR at 485.239 kb on + strand, within acrR at 485.246 kb on + strand, within acrR at 485.246 kb on + strand, within acrR at 485.255 kb on - strand, within acrR at 485.255 kb on - strand, within acrR at 485.273 kb on + strand, within acrR at 485.281 kb on + strand, within acrR at 485.283 kb on + strand, within acrR at 485.284 kb on + strand, within acrR at 485.285 kb on - strand, within acrR at 485.285 kb on - strand, within acrR at 485.289 kb on + strand, within acrR at 485.289 kb on + strand, within acrR at 485.289 kb on - strand, within acrR at 485.289 kb on - strand, within acrR at 485.299 kb on - strand, within acrR at 485.358 kb on + strand, within acrR at 485.358 kb on + strand, within acrR at 485.358 kb on + strand, within acrR at 485.358 kb on + strand, within acrR at 485.360 kb on + strand, within acrR at 485.360 kb on + strand, within acrR at 485.363 kb on + strand, within acrR at 485.364 kb on + strand, within acrR at 485.365 kb on - strand, within acrR at 485.366 kb on - strand, within acrR at 485.368 kb on - strand, within acrR at 485.374 kb on - strand, within acrR at 485.374 kb on - strand, within acrR at 485.380 kb on - strand, within acrR at 485.388 kb on - strand, within acrR at 485.394 kb on + strand, within acrR at 485.402 kb on - strand, within acrR at 485.403 kb on + strand, within acrR at 485.403 kb on + strand, within acrR at 485.408 kb on + strand, within acrR at 485.411 kb on - strand, within acrR at 485.411 kb on - strand, within acrR at 485.411 kb on - strand, within acrR at 485.416 kb on - strand, within acrR at 485.480 kb on + strand, within acrR at 485.503 kb on - strand, within acrR at 485.527 kb on + strand, within acrR at 485.574 kb on + strand at 485.575 kb on + strand at 485.575 kb on + strand at 485.582 kb on - strand at 485.582 kb on - strand at 485.584 kb on + strand at 485.586 kb on + strand at 485.586 kb on + strand at 485.586 kb on + strand at 485.586 kb on + strand at 485.586 kb on + strand at 485.586 kb on + strand at 485.586 kb on + strand at 485.592 kb on - strand at 485.594 kb on - strand at 485.596 kb on + strand at 485.601 kb on - strand at 485.604 kb on - strand at 485.606 kb on - strand at 485.608 kb on + strand at 485.608 kb on + strand at 485.616 kb on - strand at 485.616 kb on - strand at 485.665 kb on + strand at 485.763 kb on + strand at 485.763 kb on + strand at 485.776 kb on + strand at 485.782 kb on - strand at 485.837 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.00000195313 mM remove 482,650 - acrB b0462 0.69 -2.3 482,801 + acrB b0462 0.74 -1.4 482,823 - acrB b0462 0.74 -2.4 482,823 - acrB b0462 0.74 -1.9 482,870 + acrB b0462 0.76 -3.1 482,905 + +0.6 482,905 + acrB b0462 0.77 -1.3 482,932 - acrB b0462 0.78 -1.9 482,935 + acrB b0462 0.78 -3.0 482,935 + acrB b0462 0.78 -2.1 482,941 - acrB b0462 0.78 -1.6 482,969 - acrB b0462 0.79 -1.2 482,969 - acrB b0462 0.79 -1.0 482,978 + acrB b0462 0.79 -2.2 483,037 - acrB b0462 0.81 -1.7 483,037 - acrB b0462 0.81 -0.4 483,128 - acrB b0462 0.84 -2.1 483,128 - acrB b0462 0.84 -0.7 483,133 - acrB b0462 0.84 -3.0 483,301 + acrB b0462 0.90 -1.3 483,308 + acrB b0462 0.90 -2.7 483,309 - acrB b0462 0.90 -0.5 483,309 - acrB b0462 0.90 -2.8 483,316 - -2.3 483,333 - -2.3 483,358 + -2.7 483,366 - -2.0 483,376 - -1.3 483,383 + -2.2 483,383 + -0.8 483,383 - -2.0 483,393 - -2.4 483,439 - -2.1 483,451 + -1.2 483,451 + -1.3 483,515 + -1.8 483,540 + -3.4 483,584 - -2.1 483,622 + -4.0 483,635 - -0.9 483,636 - -1.4 483,636 - -1.4 483,638 - -1.9 483,672 - -1.2 483,789 + acrA b0463 0.12 -1.2 483,813 + acrA b0463 0.14 -1.8 483,813 + acrA b0463 0.14 -0.4 483,852 - acrA b0463 0.17 -0.8 483,852 - acrA b0463 0.17 -0.9 483,902 + acrA b0463 0.21 -2.2 483,903 - acrA b0463 0.21 -1.2 483,903 - acrA b0463 0.21 -2.0 483,903 - acrA b0463 0.21 -1.0 483,977 + acrA b0463 0.27 -2.6 483,987 + acrA b0463 0.28 -2.9 484,011 - acrA b0463 0.30 -3.8 484,027 + acrA b0463 0.32 -2.6 484,028 - acrA b0463 0.32 -1.7 484,028 - acrA b0463 0.32 -2.7 484,070 + acrA b0463 0.35 -0.4 484,073 + acrA b0463 0.35 -1.5 484,190 - acrA b0463 0.45 -2.4 484,202 + acrA b0463 0.46 -0.9 484,248 + acrA b0463 0.50 -1.7 484,251 - acrA b0463 0.50 -1.0 484,254 + acrA b0463 0.51 -2.9 484,261 - acrA b0463 0.51 -2.8 484,400 + acrA b0463 0.63 -0.8 484,453 + acrA b0463 0.67 -1.0 484,526 + acrA b0463 0.73 +0.2 484,526 + acrA b0463 0.73 -2.2 484,526 + acrA b0463 0.73 -0.6 484,526 + acrA b0463 0.73 -1.8 484,618 - acrA b0463 0.81 -2.1 484,618 - acrA b0463 0.81 -2.1 484,659 + acrA b0463 0.85 -1.2 484,662 - acrA b0463 0.85 -2.9 484,917 - -1.0 484,917 - -1.4 485,062 + acrR b0464 0.12 +0.3 485,071 + acrR b0464 0.13 -2.6 485,071 + acrR b0464 0.13 -0.1 485,145 - acrR b0464 0.25 -0.6 485,175 - acrR b0464 0.29 +0.4 485,193 - acrR b0464 0.32 -1.4 485,193 - acrR b0464 0.32 +0.1 485,199 + acrR b0464 0.33 -1.0 485,199 + acrR b0464 0.33 -0.2 485,239 + acrR b0464 0.39 -0.8 485,239 + acrR b0464 0.39 +0.6 485,246 + acrR b0464 0.40 +0.3 485,246 + acrR b0464 0.40 -0.1 485,255 - acrR b0464 0.42 -0.8 485,255 - acrR b0464 0.42 -0.6 485,273 + acrR b0464 0.44 +0.3 485,281 + acrR b0464 0.46 -0.6 485,283 + acrR b0464 0.46 +0.5 485,284 + acrR b0464 0.46 -0.4 485,285 - acrR b0464 0.46 -0.8 485,285 - acrR b0464 0.46 -0.4 485,289 + acrR b0464 0.47 -0.8 485,289 + acrR b0464 0.47 +0.1 485,289 - acrR b0464 0.47 -1.2 485,289 - acrR b0464 0.47 -0.6 485,299 - acrR b0464 0.48 -0.3 485,358 + acrR b0464 0.58 +0.4 485,358 + acrR b0464 0.58 +0.2 485,358 + acrR b0464 0.58 +0.4 485,358 + acrR b0464 0.58 +0.0 485,360 + acrR b0464 0.58 -2.0 485,360 + acrR b0464 0.58 -1.1 485,363 + acrR b0464 0.58 +0.0 485,364 + acrR b0464 0.58 -0.0 485,365 - acrR b0464 0.59 -1.0 485,366 - acrR b0464 0.59 -0.6 485,368 - acrR b0464 0.59 +0.6 485,374 - acrR b0464 0.60 +0.6 485,374 - acrR b0464 0.60 -1.8 485,380 - acrR b0464 0.61 -1.0 485,388 - acrR b0464 0.62 -2.2 485,394 + acrR b0464 0.63 -0.4 485,402 - acrR b0464 0.64 +0.2 485,403 + acrR b0464 0.65 -0.3 485,403 + acrR b0464 0.65 +0.9 485,408 + acrR b0464 0.65 +1.4 485,411 - acrR b0464 0.66 +1.4 485,411 - acrR b0464 0.66 -1.0 485,411 - acrR b0464 0.66 -0.8 485,416 - acrR b0464 0.67 +1.6 485,480 + acrR b0464 0.76 +0.7 485,503 - acrR b0464 0.80 -0.7 485,527 + acrR b0464 0.84 -0.3 485,574 + -1.2 485,575 + +0.7 485,575 + +0.9 485,582 - +0.4 485,582 - +0.0 485,584 + -0.1 485,586 + -0.3 485,586 + -0.1 485,586 + -0.1 485,586 + -1.0 485,586 + +0.5 485,586 + -0.5 485,586 + -0.7 485,592 - -1.0 485,594 - -0.7 485,596 + -0.2 485,601 - -0.2 485,604 - +0.1 485,606 - +0.3 485,608 + +0.6 485,608 + -1.7 485,616 - +0.3 485,616 - -1.2 485,665 + -0.7 485,763 + +0.0 485,763 + +0.8 485,776 + +0.8 485,782 - +0.3 485,837 + -0.6
Or see this region's nucleotide sequence