Experiment: LB Anaerobic with Nalidixic 0.00000195313 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hha and ybaJ are separated by 25 nucleotides ybaJ and acrB are separated by 545 nucleotides acrB and acrA are separated by 22 nucleotides
b0460: hha - modulator of gene expression, with H-NS (NCBI), at 479,314 to 479,532
hha
b0461: ybaJ - hypothetical protein (NCBI), at 479,558 to 479,932
ybaJ
b0462: acrB - multidrug efflux system protein (NCBI), at 480,478 to 483,627
acrB
b0463: acrA - multidrug efflux system (NCBI), at 483,650 to 484,843
acrA
Position (kb) 480
481
482
483
484 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 479.492 kb on - strand, within hha at 479.492 kb on - strand, within hha at 479.492 kb on - strand, within hha at 479.524 kb on + strand at 479.524 kb on + strand at 479.528 kb on + strand at 479.528 kb on - strand at 479.528 kb on - strand at 479.537 kb on + strand at 479.542 kb on - strand at 479.545 kb on - strand at 479.554 kb on + strand at 479.595 kb on - strand at 479.604 kb on - strand, within ybaJ at 479.609 kb on + strand, within ybaJ at 479.609 kb on + strand, within ybaJ at 479.630 kb on - strand, within ybaJ at 479.661 kb on - strand, within ybaJ at 479.661 kb on - strand, within ybaJ at 479.661 kb on - strand, within ybaJ at 479.661 kb on - strand, within ybaJ at 479.672 kb on - strand, within ybaJ at 479.675 kb on - strand, within ybaJ at 479.675 kb on - strand, within ybaJ at 479.675 kb on - strand, within ybaJ at 479.675 kb on - strand, within ybaJ at 479.685 kb on - strand, within ybaJ at 479.685 kb on - strand, within ybaJ at 479.685 kb on - strand, within ybaJ at 479.685 kb on - strand, within ybaJ at 479.697 kb on - strand, within ybaJ at 479.697 kb on - strand, within ybaJ at 479.703 kb on - strand, within ybaJ at 479.710 kb on + strand, within ybaJ at 479.717 kb on - strand, within ybaJ at 479.718 kb on - strand, within ybaJ at 479.718 kb on - strand, within ybaJ at 479.726 kb on - strand, within ybaJ at 479.771 kb on - strand, within ybaJ at 479.791 kb on + strand, within ybaJ at 479.799 kb on - strand, within ybaJ at 479.799 kb on - strand, within ybaJ at 479.799 kb on - strand, within ybaJ at 479.813 kb on - strand, within ybaJ at 479.813 kb on - strand, within ybaJ at 479.818 kb on - strand, within ybaJ at 479.826 kb on - strand, within ybaJ at 479.826 kb on - strand, within ybaJ at 479.836 kb on - strand, within ybaJ at 479.850 kb on - strand, within ybaJ at 479.856 kb on + strand, within ybaJ at 479.864 kb on - strand, within ybaJ at 479.886 kb on + strand, within ybaJ at 479.896 kb on - strand at 479.904 kb on - strand at 479.906 kb on - strand at 479.906 kb on - strand at 479.917 kb on - strand at 479.921 kb on + strand at 479.921 kb on + strand at 479.928 kb on + strand at 479.936 kb on - strand at 479.946 kb on - strand at 479.961 kb on - strand at 479.961 kb on - strand at 479.961 kb on - strand at 479.999 kb on + strand at 480.002 kb on - strand at 480.014 kb on + strand at 480.020 kb on + strand at 480.020 kb on + strand at 480.092 kb on + strand at 480.112 kb on + strand at 480.114 kb on - strand at 480.117 kb on + strand at 480.117 kb on + strand at 480.122 kb on + strand at 480.122 kb on + strand at 480.156 kb on + strand at 480.173 kb on + strand at 480.197 kb on + strand at 480.197 kb on + strand at 480.197 kb on + strand at 480.213 kb on + strand at 480.213 kb on + strand at 480.218 kb on + strand at 480.218 kb on + strand at 480.315 kb on + strand at 480.388 kb on + strand at 480.388 kb on + strand at 480.389 kb on - strand at 480.420 kb on + strand at 480.420 kb on + strand at 480.458 kb on - strand at 480.464 kb on - strand at 480.464 kb on - strand at 480.465 kb on + strand at 480.473 kb on - strand at 480.492 kb on + strand at 480.492 kb on + strand at 480.502 kb on - strand at 480.512 kb on - strand at 480.606 kb on + strand at 480.617 kb on - strand at 480.617 kb on - strand at 480.748 kb on - strand at 480.748 kb on - strand at 480.760 kb on + strand at 480.827 kb on - strand, within acrB at 480.827 kb on - strand, within acrB at 480.832 kb on + strand, within acrB at 480.835 kb on - strand, within acrB at 480.835 kb on - strand, within acrB at 480.835 kb on - strand, within acrB at 480.845 kb on - strand, within acrB at 480.845 kb on - strand, within acrB at 480.929 kb on - strand, within acrB at 480.949 kb on + strand, within acrB at 480.955 kb on - strand, within acrB at 480.957 kb on - strand, within acrB at 480.957 kb on - strand, within acrB at 481.005 kb on - strand, within acrB at 481.005 kb on - strand, within acrB at 481.062 kb on - strand, within acrB at 481.176 kb on - strand, within acrB at 481.176 kb on - strand, within acrB at 481.208 kb on - strand, within acrB at 481.248 kb on - strand, within acrB at 481.248 kb on - strand, within acrB at 481.253 kb on + strand, within acrB at 481.253 kb on + strand, within acrB at 481.365 kb on + strand, within acrB at 481.385 kb on + strand, within acrB at 481.388 kb on - strand, within acrB at 481.395 kb on + strand, within acrB at 481.395 kb on + strand, within acrB at 481.442 kb on + strand, within acrB at 481.442 kb on + strand, within acrB at 481.450 kb on - strand, within acrB at 481.486 kb on + strand, within acrB at 481.486 kb on + strand, within acrB at 481.559 kb on + strand, within acrB at 481.585 kb on + strand, within acrB at 481.593 kb on + strand, within acrB at 481.593 kb on + strand, within acrB at 481.601 kb on + strand, within acrB at 481.609 kb on - strand, within acrB at 481.616 kb on + strand, within acrB at 481.626 kb on + strand, within acrB at 481.762 kb on - strand, within acrB at 481.770 kb on - strand, within acrB at 481.795 kb on + strand, within acrB at 481.795 kb on + strand, within acrB at 481.860 kb on + strand, within acrB at 481.860 kb on + strand, within acrB at 481.860 kb on + strand, within acrB at 481.906 kb on + strand, within acrB at 481.955 kb on + strand, within acrB at 482.005 kb on + strand, within acrB at 482.023 kb on - strand, within acrB at 482.028 kb on - strand, within acrB at 482.028 kb on - strand, within acrB at 482.047 kb on + strand, within acrB at 482.120 kb on + strand, within acrB at 482.180 kb on + strand, within acrB at 482.208 kb on + strand, within acrB at 482.208 kb on + strand, within acrB at 482.232 kb on - strand, within acrB at 482.232 kb on - strand, within acrB at 482.317 kb on - strand, within acrB at 482.435 kb on + strand, within acrB at 482.435 kb on + strand, within acrB at 482.443 kb on - strand, within acrB at 482.580 kb on - strand, within acrB at 482.583 kb on - strand, within acrB at 482.583 kb on - strand, within acrB at 482.607 kb on - strand, within acrB at 482.607 kb on - strand, within acrB at 482.650 kb on - strand, within acrB at 482.801 kb on + strand, within acrB at 482.823 kb on - strand, within acrB at 482.823 kb on - strand, within acrB at 482.870 kb on + strand, within acrB at 482.905 kb on + strand at 482.905 kb on + strand, within acrB at 482.932 kb on - strand, within acrB at 482.935 kb on + strand, within acrB at 482.935 kb on + strand, within acrB at 482.941 kb on - strand, within acrB at 482.969 kb on - strand, within acrB at 482.969 kb on - strand, within acrB at 482.978 kb on + strand, within acrB at 483.037 kb on - strand, within acrB at 483.037 kb on - strand, within acrB at 483.128 kb on - strand, within acrB at 483.128 kb on - strand, within acrB at 483.133 kb on - strand, within acrB at 483.301 kb on + strand, within acrB at 483.308 kb on + strand, within acrB at 483.309 kb on - strand, within acrB at 483.309 kb on - strand, within acrB at 483.316 kb on - strand at 483.333 kb on - strand at 483.358 kb on + strand at 483.366 kb on - strand at 483.376 kb on - strand at 483.383 kb on + strand at 483.383 kb on + strand at 483.383 kb on - strand at 483.393 kb on - strand at 483.439 kb on - strand at 483.451 kb on + strand at 483.451 kb on + strand at 483.515 kb on + strand at 483.540 kb on + strand at 483.584 kb on - strand at 483.622 kb on + strand at 483.635 kb on - strand at 483.636 kb on - strand at 483.636 kb on - strand at 483.638 kb on - strand at 483.672 kb on - strand at 483.789 kb on + strand, within acrA at 483.813 kb on + strand, within acrA at 483.813 kb on + strand, within acrA at 483.852 kb on - strand, within acrA at 483.852 kb on - strand, within acrA at 483.902 kb on + strand, within acrA at 483.903 kb on - strand, within acrA at 483.903 kb on - strand, within acrA at 483.903 kb on - strand, within acrA at 483.977 kb on + strand, within acrA at 483.987 kb on + strand, within acrA at 484.011 kb on - strand, within acrA at 484.027 kb on + strand, within acrA at 484.028 kb on - strand, within acrA at 484.028 kb on - strand, within acrA at 484.070 kb on + strand, within acrA at 484.073 kb on + strand, within acrA at 484.190 kb on - strand, within acrA at 484.202 kb on + strand, within acrA at 484.248 kb on + strand, within acrA at 484.251 kb on - strand, within acrA at 484.254 kb on + strand, within acrA at 484.261 kb on - strand, within acrA at 484.400 kb on + strand, within acrA at 484.453 kb on + strand, within acrA at 484.526 kb on + strand, within acrA at 484.526 kb on + strand, within acrA at 484.526 kb on + strand, within acrA at 484.526 kb on + strand, within acrA at 484.618 kb on - strand, within acrA at 484.618 kb on - strand, within acrA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.00000195313 mM remove 479,492 - hha b0460 0.81 -1.2 479,492 - hha b0460 0.81 +1.0 479,492 - hha b0460 0.81 +0.6 479,524 + -1.0 479,524 + -1.7 479,528 + -0.4 479,528 - -0.6 479,528 - +0.0 479,537 + +1.6 479,542 - +1.0 479,545 - -0.6 479,554 + -0.4 479,595 - +1.0 479,604 - ybaJ b0461 0.12 -0.6 479,609 + ybaJ b0461 0.14 +0.6 479,609 + ybaJ b0461 0.14 +0.6 479,630 - ybaJ b0461 0.19 -0.1 479,661 - ybaJ b0461 0.27 -0.7 479,661 - ybaJ b0461 0.27 -0.7 479,661 - ybaJ b0461 0.27 +0.6 479,661 - ybaJ b0461 0.27 +0.6 479,672 - ybaJ b0461 0.30 +0.0 479,675 - ybaJ b0461 0.31 +1.3 479,675 - ybaJ b0461 0.31 -1.7 479,675 - ybaJ b0461 0.31 +1.3 479,675 - ybaJ b0461 0.31 +0.4 479,685 - ybaJ b0461 0.34 +0.6 479,685 - ybaJ b0461 0.34 +0.0 479,685 - ybaJ b0461 0.34 +0.6 479,685 - ybaJ b0461 0.34 -1.0 479,697 - ybaJ b0461 0.37 -1.0 479,697 - ybaJ b0461 0.37 -1.8 479,703 - ybaJ b0461 0.39 +0.6 479,710 + ybaJ b0461 0.41 +1.2 479,717 - ybaJ b0461 0.42 -0.4 479,718 - ybaJ b0461 0.43 -0.1 479,718 - ybaJ b0461 0.43 +0.6 479,726 - ybaJ b0461 0.45 -0.6 479,771 - ybaJ b0461 0.57 +0.5 479,791 + ybaJ b0461 0.62 +1.6 479,799 - ybaJ b0461 0.64 +0.9 479,799 - ybaJ b0461 0.64 +0.5 479,799 - ybaJ b0461 0.64 -1.4 479,813 - ybaJ b0461 0.68 -1.0 479,813 - ybaJ b0461 0.68 +0.3 479,818 - ybaJ b0461 0.69 +0.0 479,826 - ybaJ b0461 0.71 -0.3 479,826 - ybaJ b0461 0.71 -0.2 479,836 - ybaJ b0461 0.74 -0.7 479,850 - ybaJ b0461 0.78 -0.9 479,856 + ybaJ b0461 0.79 +0.8 479,864 - ybaJ b0461 0.82 -1.0 479,886 + ybaJ b0461 0.87 +0.3 479,896 - +0.6 479,904 - -0.5 479,906 - -0.1 479,906 - -0.2 479,917 - +0.4 479,921 + +1.0 479,921 + -0.7 479,928 + +1.0 479,936 - +2.2 479,946 - +1.6 479,961 - -1.2 479,961 - -0.8 479,961 - +0.0 479,999 + -0.2 480,002 - -1.0 480,014 + -0.5 480,020 + +0.3 480,020 + -1.4 480,092 + +0.5 480,112 + +0.0 480,114 - +0.0 480,117 + +0.2 480,117 + +0.1 480,122 + +1.4 480,122 + -0.5 480,156 + +0.3 480,173 + -0.6 480,197 + -0.1 480,197 + -0.5 480,197 + +0.4 480,213 + +0.7 480,213 + +0.8 480,218 + -0.3 480,218 + -0.1 480,315 + +1.6 480,388 + -0.3 480,388 + -0.4 480,389 - +0.6 480,420 + -0.8 480,420 + +0.3 480,458 - +0.4 480,464 - -0.7 480,464 - -2.4 480,465 + +1.6 480,473 - +0.4 480,492 + +1.6 480,492 + +1.6 480,502 - -0.7 480,512 - -1.2 480,606 + -0.7 480,617 - -1.8 480,617 - -1.8 480,748 - -2.6 480,748 - -1.6 480,760 + -1.3 480,827 - acrB b0462 0.11 -1.6 480,827 - acrB b0462 0.11 -3.0 480,832 + acrB b0462 0.11 -2.2 480,835 - acrB b0462 0.11 -2.0 480,835 - acrB b0462 0.11 -3.2 480,835 - acrB b0462 0.11 -1.5 480,845 - acrB b0462 0.12 -1.2 480,845 - acrB b0462 0.12 -1.2 480,929 - acrB b0462 0.14 -1.9 480,949 + acrB b0462 0.15 -0.6 480,955 - acrB b0462 0.15 -2.0 480,957 - acrB b0462 0.15 -3.8 480,957 - acrB b0462 0.15 -3.6 481,005 - acrB b0462 0.17 -2.2 481,005 - acrB b0462 0.17 -2.4 481,062 - acrB b0462 0.19 -1.4 481,176 - acrB b0462 0.22 -3.2 481,176 - acrB b0462 0.22 -0.9 481,208 - acrB b0462 0.23 -1.6 481,248 - acrB b0462 0.24 -2.6 481,248 - acrB b0462 0.24 -1.6 481,253 + acrB b0462 0.25 -1.0 481,253 + acrB b0462 0.25 -1.4 481,365 + acrB b0462 0.28 -1.8 481,385 + acrB b0462 0.29 -1.0 481,388 - acrB b0462 0.29 -0.7 481,395 + acrB b0462 0.29 -1.7 481,395 + acrB b0462 0.29 -2.2 481,442 + acrB b0462 0.31 -0.7 481,442 + acrB b0462 0.31 -0.7 481,450 - acrB b0462 0.31 -2.2 481,486 + acrB b0462 0.32 -1.0 481,486 + acrB b0462 0.32 -0.9 481,559 + acrB b0462 0.34 -1.7 481,585 + acrB b0462 0.35 -3.9 481,593 + acrB b0462 0.35 -2.8 481,593 + acrB b0462 0.35 -2.3 481,601 + acrB b0462 0.36 -1.4 481,609 - acrB b0462 0.36 -2.8 481,616 + acrB b0462 0.36 -3.3 481,626 + acrB b0462 0.36 -1.7 481,762 - acrB b0462 0.41 -1.9 481,770 - acrB b0462 0.41 -3.8 481,795 + acrB b0462 0.42 -1.1 481,795 + acrB b0462 0.42 -2.1 481,860 + acrB b0462 0.44 -0.6 481,860 + acrB b0462 0.44 -1.8 481,860 + acrB b0462 0.44 -1.3 481,906 + acrB b0462 0.45 -1.6 481,955 + acrB b0462 0.47 -2.7 482,005 + acrB b0462 0.48 -1.3 482,023 - acrB b0462 0.49 -1.7 482,028 - acrB b0462 0.49 -1.8 482,028 - acrB b0462 0.49 -1.0 482,047 + acrB b0462 0.50 -0.8 482,120 + acrB b0462 0.52 -3.9 482,180 + acrB b0462 0.54 -1.0 482,208 + acrB b0462 0.55 -0.2 482,208 + acrB b0462 0.55 -0.6 482,232 - acrB b0462 0.56 -2.0 482,232 - acrB b0462 0.56 -1.1 482,317 - acrB b0462 0.58 -1.0 482,435 + acrB b0462 0.62 -2.4 482,435 + acrB b0462 0.62 -0.3 482,443 - acrB b0462 0.62 -2.6 482,580 - acrB b0462 0.67 -0.3 482,583 - acrB b0462 0.67 -3.6 482,583 - acrB b0462 0.67 -0.9 482,607 - acrB b0462 0.68 -1.9 482,607 - acrB b0462 0.68 -2.2 482,650 - acrB b0462 0.69 -2.3 482,801 + acrB b0462 0.74 -1.4 482,823 - acrB b0462 0.74 -2.4 482,823 - acrB b0462 0.74 -1.9 482,870 + acrB b0462 0.76 -3.1 482,905 + +0.6 482,905 + acrB b0462 0.77 -1.3 482,932 - acrB b0462 0.78 -1.9 482,935 + acrB b0462 0.78 -3.0 482,935 + acrB b0462 0.78 -2.1 482,941 - acrB b0462 0.78 -1.6 482,969 - acrB b0462 0.79 -1.2 482,969 - acrB b0462 0.79 -1.0 482,978 + acrB b0462 0.79 -2.2 483,037 - acrB b0462 0.81 -1.7 483,037 - acrB b0462 0.81 -0.4 483,128 - acrB b0462 0.84 -2.1 483,128 - acrB b0462 0.84 -0.7 483,133 - acrB b0462 0.84 -3.0 483,301 + acrB b0462 0.90 -1.3 483,308 + acrB b0462 0.90 -2.7 483,309 - acrB b0462 0.90 -0.5 483,309 - acrB b0462 0.90 -2.8 483,316 - -2.3 483,333 - -2.3 483,358 + -2.7 483,366 - -2.0 483,376 - -1.3 483,383 + -2.2 483,383 + -0.8 483,383 - -2.0 483,393 - -2.4 483,439 - -2.1 483,451 + -1.2 483,451 + -1.3 483,515 + -1.8 483,540 + -3.4 483,584 - -2.1 483,622 + -4.0 483,635 - -0.9 483,636 - -1.4 483,636 - -1.4 483,638 - -1.9 483,672 - -1.2 483,789 + acrA b0463 0.12 -1.2 483,813 + acrA b0463 0.14 -1.8 483,813 + acrA b0463 0.14 -0.4 483,852 - acrA b0463 0.17 -0.8 483,852 - acrA b0463 0.17 -0.9 483,902 + acrA b0463 0.21 -2.2 483,903 - acrA b0463 0.21 -1.2 483,903 - acrA b0463 0.21 -2.0 483,903 - acrA b0463 0.21 -1.0 483,977 + acrA b0463 0.27 -2.6 483,987 + acrA b0463 0.28 -2.9 484,011 - acrA b0463 0.30 -3.8 484,027 + acrA b0463 0.32 -2.6 484,028 - acrA b0463 0.32 -1.7 484,028 - acrA b0463 0.32 -2.7 484,070 + acrA b0463 0.35 -0.4 484,073 + acrA b0463 0.35 -1.5 484,190 - acrA b0463 0.45 -2.4 484,202 + acrA b0463 0.46 -0.9 484,248 + acrA b0463 0.50 -1.7 484,251 - acrA b0463 0.50 -1.0 484,254 + acrA b0463 0.51 -2.9 484,261 - acrA b0463 0.51 -2.8 484,400 + acrA b0463 0.63 -0.8 484,453 + acrA b0463 0.67 -1.0 484,526 + acrA b0463 0.73 +0.2 484,526 + acrA b0463 0.73 -2.2 484,526 + acrA b0463 0.73 -0.6 484,526 + acrA b0463 0.73 -1.8 484,618 - acrA b0463 0.81 -2.1 484,618 - acrA b0463 0.81 -2.1
Or see this region's nucleotide sequence