Experiment: LB Anaerobic with Azlocillin 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yahI and yahJ are separated by 9 nucleotides yahJ and yahK are separated by 376 nucleotides
b0323: yahI - predicted carbamate kinase-like protein (NCBI), at 339,389 to 340,339
yahI
b0324: yahJ - deaminase (NCBI), at 340,349 to 341,731
yahJ
b0325: yahK - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI), at 342,108 to 343,157
yahK
Position (kb) 340
341
342 Strain fitness (log2 ratio) -2
-1
0
1 at 339.378 kb on - strand at 339.390 kb on - strand at 339.390 kb on - strand at 339.411 kb on - strand at 339.431 kb on + strand at 339.444 kb on - strand at 339.565 kb on + strand, within yahI at 339.565 kb on + strand, within yahI at 339.630 kb on + strand, within yahI at 339.630 kb on + strand, within yahI at 339.741 kb on - strand, within yahI at 339.798 kb on + strand, within yahI at 339.806 kb on - strand, within yahI at 340.025 kb on + strand, within yahI at 340.025 kb on + strand, within yahI at 340.047 kb on - strand, within yahI at 340.170 kb on - strand, within yahI at 340.174 kb on - strand, within yahI at 340.174 kb on - strand, within yahI at 340.257 kb on + strand at 340.257 kb on + strand at 340.320 kb on + strand at 340.320 kb on + strand at 340.394 kb on + strand at 340.448 kb on + strand at 340.461 kb on + strand at 340.522 kb on - strand, within yahJ at 340.573 kb on - strand, within yahJ at 340.573 kb on - strand, within yahJ at 340.675 kb on + strand, within yahJ at 340.675 kb on + strand, within yahJ at 340.776 kb on + strand, within yahJ at 340.952 kb on + strand, within yahJ at 341.087 kb on - strand, within yahJ at 341.087 kb on - strand, within yahJ at 341.124 kb on + strand, within yahJ at 341.139 kb on - strand, within yahJ at 341.141 kb on + strand, within yahJ at 341.385 kb on - strand, within yahJ at 341.385 kb on - strand, within yahJ at 341.530 kb on + strand, within yahJ at 341.530 kb on + strand, within yahJ at 341.632 kb on + strand at 341.649 kb on - strand at 341.708 kb on + strand at 341.983 kb on - strand at 341.997 kb on + strand at 341.997 kb on - strand at 342.031 kb on + strand at 342.082 kb on - strand at 342.086 kb on - strand at 342.091 kb on + strand at 342.091 kb on + strand at 342.136 kb on - strand at 342.169 kb on + strand at 342.219 kb on + strand, within yahK at 342.219 kb on + strand, within yahK at 342.257 kb on - strand, within yahK at 342.276 kb on - strand, within yahK at 342.322 kb on - strand, within yahK at 342.347 kb on + strand, within yahK at 342.361 kb on + strand, within yahK at 342.422 kb on + strand, within yahK at 342.445 kb on + strand, within yahK at 342.488 kb on - strand, within yahK at 342.493 kb on + strand, within yahK at 342.510 kb on + strand, within yahK at 342.525 kb on + strand, within yahK at 342.525 kb on + strand, within yahK at 342.714 kb on + strand, within yahK at 342.714 kb on + strand, within yahK
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Azlocillin 0.000015625 mM remove 339,378 - +0.0 339,390 - +0.9 339,390 - -0.9 339,411 - -0.1 339,431 + +0.7 339,444 - +0.8 339,565 + yahI b0323 0.19 +1.5 339,565 + yahI b0323 0.19 +0.4 339,630 + yahI b0323 0.25 -0.1 339,630 + yahI b0323 0.25 +0.4 339,741 - yahI b0323 0.37 -0.7 339,798 + yahI b0323 0.43 +0.3 339,806 - yahI b0323 0.44 +0.0 340,025 + yahI b0323 0.67 +0.6 340,025 + yahI b0323 0.67 +1.5 340,047 - yahI b0323 0.69 +0.1 340,170 - yahI b0323 0.82 +0.3 340,174 - yahI b0323 0.83 -1.0 340,174 - yahI b0323 0.83 +0.0 340,257 + +1.3 340,257 + -0.3 340,320 + -0.3 340,320 + -0.3 340,394 + +0.3 340,448 + -0.3 340,461 + -2.5 340,522 - yahJ b0324 0.13 -0.8 340,573 - yahJ b0324 0.16 -2.7 340,573 - yahJ b0324 0.16 -0.2 340,675 + yahJ b0324 0.24 -0.8 340,675 + yahJ b0324 0.24 -0.9 340,776 + yahJ b0324 0.31 -0.5 340,952 + yahJ b0324 0.44 -0.6 341,087 - yahJ b0324 0.53 -0.2 341,087 - yahJ b0324 0.53 +0.3 341,124 + yahJ b0324 0.56 -0.2 341,139 - yahJ b0324 0.57 -0.5 341,141 + yahJ b0324 0.57 -0.3 341,385 - yahJ b0324 0.75 -0.0 341,385 - yahJ b0324 0.75 -0.3 341,530 + yahJ b0324 0.85 -1.3 341,530 + yahJ b0324 0.85 +0.1 341,632 + -0.8 341,649 - +0.5 341,708 + +0.0 341,983 - -0.2 341,997 + -0.0 341,997 - -0.1 342,031 + +0.2 342,082 - -1.2 342,086 - -0.0 342,091 + +1.7 342,091 + +1.1 342,136 - +0.3 342,169 + -0.2 342,219 + yahK b0325 0.11 -0.3 342,219 + yahK b0325 0.11 +0.8 342,257 - yahK b0325 0.14 -1.8 342,276 - yahK b0325 0.16 -0.3 342,322 - yahK b0325 0.20 +0.5 342,347 + yahK b0325 0.23 +0.6 342,361 + yahK b0325 0.24 -0.1 342,422 + yahK b0325 0.30 +0.2 342,445 + yahK b0325 0.32 -0.0 342,488 - yahK b0325 0.36 +0.7 342,493 + yahK b0325 0.37 +0.5 342,510 + yahK b0325 0.38 +0.3 342,525 + yahK b0325 0.40 +0.1 342,525 + yahK b0325 0.40 +0.3 342,714 + yahK b0325 0.58 -0.5 342,714 + yahK b0325 0.58 +0.7
Or see this region's nucleotide sequence