Experiment: LB Anaerobic with Azlocillin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dicF and dicB are separated by 174 nucleotides dicB and ydfD overlap by 4 nucleotides ydfD and ydfE are separated by 92 nucleotides ydfE and insD are separated by 2 nucleotides insD and intQ are separated by 13 nucleotides
b1574: dicF - antisense RNA DicF (NCBI), at 1,647,406 to 1,647,458
dicF
b1575: dicB - Qin prophage; cell division inhibition protein (NCBI), at 1,647,633 to 1,647,821
dicB
b1576: ydfD - Qin prophage; predicted protein (NCBI), at 1,647,818 to 1,648,009
ydfD
b1577: ydfE - Qin prophage; predicted protein (RefSeq), at 1,648,102 to 1,648,866
ydfE
b1578: insD - Qin prophage; IS2 insertion element transposase InsAB', C-ter fragment (pseudogene) (RefSeq), at 1,648,869 to 1,649,561
insD
b1579: intQ - Qin prophage; predicted defective integrase (RefSeq), at 1,649,575 to 1,650,732
intQ
Position (kb)
1648
1649 Strain fitness (log2 ratio)
-1
0
1
2 at 1647.366 kb on - strand at 1647.500 kb on - strand at 1647.653 kb on + strand, within dicB at 1647.700 kb on + strand, within dicB at 1647.709 kb on + strand, within dicB at 1647.775 kb on - strand, within dicB at 1647.880 kb on - strand, within ydfD at 1647.900 kb on - strand, within ydfD at 1647.957 kb on - strand, within ydfD at 1648.158 kb on - strand at 1648.158 kb on - strand at 1648.173 kb on + strand at 1648.173 kb on + strand at 1648.176 kb on - strand at 1648.176 kb on - strand at 1648.191 kb on - strand, within ydfE at 1648.250 kb on - strand, within ydfE at 1648.252 kb on + strand, within ydfE at 1648.260 kb on - strand, within ydfE at 1648.260 kb on - strand, within ydfE at 1648.260 kb on - strand, within ydfE at 1648.260 kb on - strand, within ydfE at 1648.297 kb on + strand, within ydfE at 1648.301 kb on - strand, within ydfE at 1648.301 kb on - strand, within ydfE at 1648.305 kb on - strand, within ydfE at 1648.306 kb on + strand, within ydfE at 1648.333 kb on + strand, within ydfE at 1648.357 kb on + strand, within ydfE at 1648.391 kb on - strand, within ydfE at 1648.391 kb on - strand, within ydfE at 1648.446 kb on - strand, within ydfE at 1648.725 kb on + strand, within ydfE at 1649.529 kb on + strand at 1649.556 kb on + strand at 1649.573 kb on + strand at 1649.573 kb on + strand at 1649.578 kb on + strand at 1649.757 kb on - strand, within intQ at 1649.759 kb on - strand, within intQ at 1649.864 kb on + strand, within intQ
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Azlocillin 0.00003125 mM remove 1,647,366 - +0.0 1,647,500 - +0.6 1,647,653 + dicB b1575 0.11 -1.4 1,647,700 + dicB b1575 0.35 +0.0 1,647,709 + dicB b1575 0.40 +0.4 1,647,775 - dicB b1575 0.75 +1.6 1,647,880 - ydfD b1576 0.32 +1.6 1,647,900 - ydfD b1576 0.43 +1.6 1,647,957 - ydfD b1576 0.72 +1.6 1,648,158 - +0.0 1,648,158 - +0.6 1,648,173 + +1.6 1,648,173 + +0.0 1,648,176 - +1.6 1,648,176 - -0.4 1,648,191 - ydfE b1577 0.12 -0.4 1,648,250 - ydfE b1577 0.19 +0.9 1,648,252 + ydfE b1577 0.20 +0.0 1,648,260 - ydfE b1577 0.21 -0.2 1,648,260 - ydfE b1577 0.21 -0.1 1,648,260 - ydfE b1577 0.21 +0.2 1,648,260 - ydfE b1577 0.21 +2.0 1,648,297 + ydfE b1577 0.25 +0.6 1,648,301 - ydfE b1577 0.26 +1.0 1,648,301 - ydfE b1577 0.26 +1.0 1,648,305 - ydfE b1577 0.27 +0.4 1,648,306 + ydfE b1577 0.27 -1.0 1,648,333 + ydfE b1577 0.30 +0.6 1,648,357 + ydfE b1577 0.33 +0.6 1,648,391 - ydfE b1577 0.38 +0.3 1,648,391 - ydfE b1577 0.38 +0.9 1,648,446 - ydfE b1577 0.45 +0.6 1,648,725 + ydfE b1577 0.81 +0.2 1,649,529 + +0.6 1,649,556 + +0.4 1,649,573 + +1.6 1,649,573 + -0.4 1,649,578 + -0.7 1,649,757 - intQ b1579 0.16 +1.6 1,649,759 - intQ b1579 0.16 +1.6 1,649,864 + intQ b1579 0.25 +1.6
Or see this region's nucleotide sequence