Strain Fitness in Escherichia coli BW25113 around b1443

Experiment: LB Anaerobic with Azlocillin 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydcT and ydcU are separated by 0 nucleotidesydcU and ydcV overlap by 11 nucleotidesydcV and ydcW are separated by 21 nucleotides b1441: ydcT - predicted spermidine/putrescine transporter subunit (NCBI), at 1,510,841 to 1,511,854 ydcT b1442: ydcU - predicted spermidine/putrescine transporter subunit (NCBI), at 1,511,855 to 1,512,796 ydcU b1443: ydcV - predicted spermidine/putrescine transporter subunit (NCBI), at 1,512,786 to 1,513,580 ydcV b1444: ydcW - medium chain aldehyde dehydrogenase (NCBI), at 1,513,602 to 1,515,026 ydcW Position (kb) 1512 1513 1514Strain fitness (log2 ratio) -2 -1 0 1at 1511.886 kb on + strandat 1511.886 kb on + strandat 1512.028 kb on - strand, within ydcUat 1512.111 kb on - strand, within ydcUat 1512.111 kb on - strand, within ydcUat 1512.133 kb on + strand, within ydcUat 1512.192 kb on - strand, within ydcUat 1512.192 kb on - strand, within ydcUat 1512.194 kb on + strand, within ydcUat 1512.269 kb on - strand, within ydcUat 1512.470 kb on - strand, within ydcUat 1512.754 kb on - strandat 1512.754 kb on - strandat 1512.889 kb on - strand, within ydcVat 1512.964 kb on - strand, within ydcVat 1512.964 kb on - strand, within ydcVat 1513.150 kb on - strand, within ydcVat 1513.161 kb on - strand, within ydcVat 1513.161 kb on - strand, within ydcVat 1513.229 kb on + strand, within ydcVat 1513.229 kb on + strand, within ydcVat 1514.101 kb on + strand, within ydcWat 1514.310 kb on - strand, within ydcWat 1514.401 kb on + strand, within ydcWat 1514.401 kb on + strand, within ydcWat 1514.435 kb on + strand, within ydcWat 1514.503 kb on + strand, within ydcW

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Azlocillin 0.00003125 mM
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1,511,886 + +0.1
1,511,886 + -0.7
1,512,028 - ydcU b1442 0.18 +1.0
1,512,111 - ydcU b1442 0.27 -1.1
1,512,111 - ydcU b1442 0.27 -0.0
1,512,133 + ydcU b1442 0.30 -0.5
1,512,192 - ydcU b1442 0.36 +0.0
1,512,192 - ydcU b1442 0.36 +0.1
1,512,194 + ydcU b1442 0.36 +0.4
1,512,269 - ydcU b1442 0.44 -0.4
1,512,470 - ydcU b1442 0.65 -1.0
1,512,754 - +0.0
1,512,754 - -0.6
1,512,889 - ydcV b1443 0.13 +1.1
1,512,964 - ydcV b1443 0.22 +1.4
1,512,964 - ydcV b1443 0.22 -1.9
1,513,150 - ydcV b1443 0.46 +0.1
1,513,161 - ydcV b1443 0.47 -0.3
1,513,161 - ydcV b1443 0.47 +0.3
1,513,229 + ydcV b1443 0.56 +0.9
1,513,229 + ydcV b1443 0.56 +0.9
1,514,101 + ydcW b1444 0.35 -0.7
1,514,310 - ydcW b1444 0.50 -0.7
1,514,401 + ydcW b1444 0.56 +0.1
1,514,401 + ydcW b1444 0.56 +0.4
1,514,435 + ydcW b1444 0.58 +0.0
1,514,503 + ydcW b1444 0.63 -0.4

Or see this region's nucleotide sequence