Experiment: LB Anaerobic with Azlocillin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mraW and ftsL overlap by 4 nucleotides ftsL and ftsI are separated by 15 nucleotides ftsI and murE overlap by 14 nucleotides
b0082: mraW - S-adenosyl-methyltransferase (NCBI), at 90,094 to 91,035
mraW
b0083: ftsL - membrane bound cell division protein at septum containing leucine zipper motif (NCBI), at 91,032 to 91,397
ftsL
b0084: ftsI - transpeptidase involved in septal peptidoglycan synthesis (penicillin-binding protein 3) (NCBI), at 91,413 to 93,179
ftsI
b0085: murE - UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase (NCBI), at 93,166 to 94,653
murE
Position (kb) 91
92
93
94 Strain fitness (log2 ratio) -1
0
1
2 at 90.473 kb on + strand, within mraW at 90.518 kb on + strand, within mraW at 90.544 kb on + strand, within mraW at 90.681 kb on + strand, within mraW at 90.701 kb on + strand, within mraW at 90.783 kb on + strand, within mraW at 90.887 kb on + strand, within mraW at 90.896 kb on + strand, within mraW at 90.899 kb on + strand, within mraW at 90.979 kb on + strand at 91.008 kb on + strand at 91.020 kb on + strand at 91.020 kb on + strand at 91.020 kb on + strand at 91.028 kb on + strand at 91.028 kb on + strand at 91.357 kb on + strand at 91.362 kb on + strand at 91.378 kb on + strand at 93.112 kb on + strand at 93.112 kb on + strand at 93.112 kb on + strand at 93.113 kb on + strand at 93.113 kb on + strand at 93.113 kb on + strand at 93.153 kb on + strand at 93.219 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Azlocillin 0.00003125 mM remove 90,473 + mraW b0082 0.40 -0.7 90,518 + mraW b0082 0.45 -0.3 90,544 + mraW b0082 0.48 +0.6 90,681 + mraW b0082 0.62 +0.8 90,701 + mraW b0082 0.64 -0.6 90,783 + mraW b0082 0.73 +0.4 90,887 + mraW b0082 0.84 -0.8 90,896 + mraW b0082 0.85 +0.4 90,899 + mraW b0082 0.85 -0.3 90,979 + +2.0 91,008 + -1.3 91,020 + -0.8 91,020 + -0.3 91,020 + +0.8 91,028 + -1.5 91,028 + -0.3 91,357 + -0.0 91,362 + -0.3 91,378 + -0.3 93,112 + +0.1 93,112 + +1.6 93,112 + -0.0 93,113 + +0.3 93,113 + +0.3 93,113 + +0.9 93,153 + -0.2 93,219 + -0.6
Or see this region's nucleotide sequence