Experiment: LB Anaerobic with Oxytetracycline 0.00000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhhA and ugpQ overlap by 14 nucleotides ugpQ and ugpC overlap by 4 nucleotides ugpC and ugpE are separated by 1 nucleotides ugpE and ugpA overlap by 4 nucleotides
b3448: yhhA - hypothetical protein (NCBI), at 3,584,966 to 3,585,406
yhhA
b3449: ugpQ - cytoplasmic glycerophosphodiester phosphodiesterase (NCBI), at 3,585,393 to 3,586,136
ugpQ
b3450: ugpC - ATP-binding component of sn-glycerol 3-phosphate transport system (VIMSS), at 3,586,133 to 3,587,203
ugpC
b3451: ugpE - glycerol-3-phosphate transporter subunit (NCBI), at 3,587,205 to 3,588,050
ugpE
b3452: ugpA - glycerol-3-phosphate transporter subunit (NCBI), at 3,588,047 to 3,588,934
ugpA
Position (kb)
3586
3587
3588 Strain fitness (log2 ratio)
-2
-1
0
1 at 3585.164 kb on - strand, within yhhA at 3585.166 kb on + strand, within yhhA at 3585.166 kb on + strand, within yhhA at 3585.316 kb on + strand, within yhhA at 3585.366 kb on - strand at 3585.369 kb on - strand at 3585.383 kb on + strand at 3585.431 kb on + strand at 3585.436 kb on + strand at 3585.436 kb on + strand at 3585.436 kb on + strand at 3585.462 kb on - strand at 3585.485 kb on - strand, within ugpQ at 3585.485 kb on - strand, within ugpQ at 3585.503 kb on + strand, within ugpQ at 3585.508 kb on - strand, within ugpQ at 3585.508 kb on - strand, within ugpQ at 3585.531 kb on - strand, within ugpQ at 3585.547 kb on + strand, within ugpQ at 3585.673 kb on + strand, within ugpQ at 3585.673 kb on + strand, within ugpQ at 3585.702 kb on - strand, within ugpQ at 3585.702 kb on - strand, within ugpQ at 3585.838 kb on - strand, within ugpQ at 3585.838 kb on - strand, within ugpQ at 3585.859 kb on - strand, within ugpQ at 3586.093 kb on - strand at 3586.198 kb on - strand at 3586.198 kb on - strand at 3586.312 kb on - strand, within ugpC at 3586.320 kb on - strand, within ugpC at 3586.396 kb on + strand, within ugpC at 3586.396 kb on + strand, within ugpC at 3586.418 kb on - strand, within ugpC at 3586.418 kb on - strand, within ugpC at 3586.551 kb on + strand, within ugpC at 3586.564 kb on - strand, within ugpC at 3586.564 kb on - strand, within ugpC at 3586.600 kb on - strand, within ugpC at 3586.619 kb on - strand, within ugpC at 3586.619 kb on - strand, within ugpC at 3586.635 kb on - strand, within ugpC at 3586.635 kb on - strand, within ugpC at 3586.675 kb on + strand, within ugpC at 3586.699 kb on + strand, within ugpC at 3586.699 kb on + strand, within ugpC at 3586.718 kb on - strand, within ugpC at 3586.773 kb on + strand, within ugpC at 3586.773 kb on + strand, within ugpC at 3586.834 kb on + strand, within ugpC at 3586.834 kb on + strand, within ugpC at 3586.924 kb on - strand, within ugpC at 3586.929 kb on + strand, within ugpC at 3586.936 kb on + strand, within ugpC at 3586.936 kb on + strand, within ugpC at 3586.952 kb on + strand, within ugpC at 3586.952 kb on + strand, within ugpC at 3587.229 kb on + strand at 3587.229 kb on + strand at 3587.298 kb on - strand, within ugpE at 3587.315 kb on - strand, within ugpE at 3587.383 kb on + strand, within ugpE at 3587.669 kb on - strand, within ugpE at 3587.753 kb on + strand, within ugpE at 3587.794 kb on - strand, within ugpE at 3587.822 kb on - strand, within ugpE at 3587.822 kb on - strand, within ugpE at 3587.849 kb on + strand, within ugpE at 3587.857 kb on - strand, within ugpE at 3587.908 kb on + strand, within ugpE at 3587.935 kb on + strand, within ugpE at 3587.970 kb on - strand at 3588.024 kb on + strand at 3588.047 kb on - strand at 3588.047 kb on - strand at 3588.067 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Oxytetracycline 0.00000015625 mM remove 3,585,164 - yhhA b3448 0.45 -0.1 3,585,166 + yhhA b3448 0.45 -0.5 3,585,166 + yhhA b3448 0.45 +0.5 3,585,316 + yhhA b3448 0.79 -0.0 3,585,366 - -0.9 3,585,369 - +0.5 3,585,383 + -0.0 3,585,431 + +0.3 3,585,436 + +1.3 3,585,436 + -0.7 3,585,436 + +0.1 3,585,462 - +0.5 3,585,485 - ugpQ b3449 0.12 -1.0 3,585,485 - ugpQ b3449 0.12 +0.1 3,585,503 + ugpQ b3449 0.15 +0.8 3,585,508 - ugpQ b3449 0.15 +0.2 3,585,508 - ugpQ b3449 0.15 -0.5 3,585,531 - ugpQ b3449 0.19 -0.4 3,585,547 + ugpQ b3449 0.21 +0.5 3,585,673 + ugpQ b3449 0.38 +0.3 3,585,673 + ugpQ b3449 0.38 +0.5 3,585,702 - ugpQ b3449 0.42 +0.3 3,585,702 - ugpQ b3449 0.42 +0.8 3,585,838 - ugpQ b3449 0.60 -0.2 3,585,838 - ugpQ b3449 0.60 +1.6 3,585,859 - ugpQ b3449 0.63 -0.5 3,586,093 - +0.2 3,586,198 - -1.1 3,586,198 - -0.5 3,586,312 - ugpC b3450 0.17 -1.6 3,586,320 - ugpC b3450 0.17 -1.3 3,586,396 + ugpC b3450 0.25 -0.4 3,586,396 + ugpC b3450 0.25 -0.5 3,586,418 - ugpC b3450 0.27 -0.6 3,586,418 - ugpC b3450 0.27 -1.4 3,586,551 + ugpC b3450 0.39 -0.0 3,586,564 - ugpC b3450 0.40 -1.2 3,586,564 - ugpC b3450 0.40 +0.1 3,586,600 - ugpC b3450 0.44 +0.6 3,586,619 - ugpC b3450 0.45 -0.3 3,586,619 - ugpC b3450 0.45 -0.3 3,586,635 - ugpC b3450 0.47 -0.4 3,586,635 - ugpC b3450 0.47 -0.9 3,586,675 + ugpC b3450 0.51 -0.1 3,586,699 + ugpC b3450 0.53 +0.1 3,586,699 + ugpC b3450 0.53 -0.2 3,586,718 - ugpC b3450 0.55 -0.8 3,586,773 + ugpC b3450 0.60 -0.1 3,586,773 + ugpC b3450 0.60 +0.8 3,586,834 + ugpC b3450 0.65 -0.8 3,586,834 + ugpC b3450 0.65 -0.8 3,586,924 - ugpC b3450 0.74 -0.5 3,586,929 + ugpC b3450 0.74 -1.3 3,586,936 + ugpC b3450 0.75 +0.6 3,586,936 + ugpC b3450 0.75 -1.1 3,586,952 + ugpC b3450 0.76 -1.9 3,586,952 + ugpC b3450 0.76 +0.1 3,587,229 + -1.9 3,587,229 + +0.2 3,587,298 - ugpE b3451 0.11 -0.1 3,587,315 - ugpE b3451 0.13 +0.4 3,587,383 + ugpE b3451 0.21 -0.1 3,587,669 - ugpE b3451 0.55 -0.6 3,587,753 + ugpE b3451 0.65 +0.7 3,587,794 - ugpE b3451 0.70 +0.5 3,587,822 - ugpE b3451 0.73 +0.5 3,587,822 - ugpE b3451 0.73 +0.2 3,587,849 + ugpE b3451 0.76 +0.8 3,587,857 - ugpE b3451 0.77 -0.4 3,587,908 + ugpE b3451 0.83 -0.4 3,587,935 + ugpE b3451 0.86 -0.4 3,587,970 - -0.1 3,588,024 + +1.0 3,588,047 - -0.1 3,588,047 - -0.5 3,588,067 + -0.0
Or see this region's nucleotide sequence