Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prc and proQ are separated by 19 nucleotides proQ and yebR are separated by 96 nucleotides yebR and yebS are separated by 129 nucleotides
b1830: prc - carboxy-terminal protease for penicillin-binding protein 3 (NCBI), at 1,910,792 to 1,912,840
prc
b1831: proQ - putative solute/DNA competence effector (NCBI), at 1,912,860 to 1,913,558
proQ
b1832: yebR - free methionine-(R)-sulfoxide reductase (RefSeq), at 1,913,655 to 1,914,152
yebR
b1833: yebS - conserved inner membrane protein (NCBI), at 1,914,282 to 1,915,565
yebS
Position (kb)
1912
1913
1914 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1912.082 kb on + strand, within prc at 1912.123 kb on - strand, within prc at 1912.140 kb on + strand, within prc at 1912.140 kb on + strand, within prc at 1912.166 kb on + strand, within prc at 1912.166 kb on + strand, within prc at 1912.759 kb on + strand at 1912.759 kb on + strand at 1912.794 kb on - strand at 1912.972 kb on + strand, within proQ at 1912.972 kb on + strand, within proQ at 1913.231 kb on - strand, within proQ at 1913.263 kb on - strand, within proQ at 1913.263 kb on - strand, within proQ at 1913.405 kb on + strand, within proQ at 1913.405 kb on + strand, within proQ at 1913.413 kb on - strand, within proQ at 1913.725 kb on - strand, within yebR at 1913.752 kb on + strand, within yebR at 1913.752 kb on + strand, within yebR at 1913.760 kb on - strand, within yebR at 1913.790 kb on - strand, within yebR at 1913.790 kb on - strand, within yebR at 1913.864 kb on - strand, within yebR at 1913.864 kb on - strand, within yebR at 1913.964 kb on - strand, within yebR at 1913.964 kb on - strand, within yebR at 1913.965 kb on - strand, within yebR at 1913.984 kb on + strand, within yebR at 1914.464 kb on + strand, within yebS
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 1,912,082 + prc b1830 0.63 -2.5 1,912,123 - prc b1830 0.65 -1.4 1,912,140 + prc b1830 0.66 -0.4 1,912,140 + prc b1830 0.66 -0.7 1,912,166 + prc b1830 0.67 -1.6 1,912,166 + prc b1830 0.67 -2.0 1,912,759 + -2.0 1,912,759 + -2.7 1,912,794 - -1.0 1,912,972 + proQ b1831 0.16 -2.7 1,912,972 + proQ b1831 0.16 +0.3 1,913,231 - proQ b1831 0.53 -0.8 1,913,263 - proQ b1831 0.58 -1.4 1,913,263 - proQ b1831 0.58 +0.4 1,913,405 + proQ b1831 0.78 -1.0 1,913,405 + proQ b1831 0.78 +0.6 1,913,413 - proQ b1831 0.79 -2.4 1,913,725 - yebR b1832 0.14 -1.1 1,913,752 + yebR b1832 0.19 +0.4 1,913,752 + yebR b1832 0.19 +0.4 1,913,760 - yebR b1832 0.21 +2.0 1,913,790 - yebR b1832 0.27 -1.1 1,913,790 - yebR b1832 0.27 +0.3 1,913,864 - yebR b1832 0.42 +0.4 1,913,864 - yebR b1832 0.42 +0.4 1,913,964 - yebR b1832 0.62 +1.4 1,913,964 - yebR b1832 0.62 +1.3 1,913,965 - yebR b1832 0.62 -0.5 1,913,984 + yebR b1832 0.66 -0.4 1,914,464 + yebS b1833 0.14 +0.4
Or see this region's nucleotide sequence