Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frwC and frwB are separated by 14 nucleotides frwB and pflD are separated by 50 nucleotides pflD and pflC overlap by 35 nucleotides pflC and frwD are separated by 1 nucleotides
b3949: frwC - predicted enzyme IIC component of PTS (NCBI), at 4,140,553 to 4,141,632
frwC
b3950: frwB - predicted enzyme IIB component of PTS (NCBI), at 4,141,647 to 4,141,967
frwB
b3951: pflD - predicted formate acetyltransferase 2 (pyruvate formate lyase II) (NCBI), at 4,142,018 to 4,144,315
pflD
b3952: pflC - probable pyruvate formate lyase activating enzyme 2 (VIMSS), at 4,144,281 to 4,145,159
pflC
b3953: frwD - predicted enzyme IIB component of PTS (NCBI), at 4,145,161 to 4,145,502
frwD
Position (kb)
4142
4143
4144
4145 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4141.051 kb on - strand, within frwC at 4141.066 kb on + strand, within frwC at 4141.069 kb on + strand, within frwC at 4141.069 kb on + strand, within frwC at 4141.069 kb on + strand, within frwC at 4141.092 kb on - strand, within frwC at 4141.105 kb on + strand, within frwC at 4141.130 kb on - strand, within frwC at 4141.133 kb on + strand, within frwC at 4141.154 kb on + strand, within frwC at 4141.167 kb on + strand, within frwC at 4141.191 kb on - strand, within frwC at 4141.194 kb on - strand, within frwC at 4141.194 kb on - strand, within frwC at 4141.218 kb on + strand, within frwC at 4141.218 kb on + strand, within frwC at 4141.255 kb on + strand, within frwC at 4141.255 kb on + strand, within frwC at 4141.311 kb on + strand, within frwC at 4141.311 kb on + strand, within frwC at 4141.320 kb on - strand, within frwC at 4141.320 kb on - strand, within frwC at 4141.320 kb on - strand, within frwC at 4141.441 kb on - strand, within frwC at 4141.448 kb on - strand, within frwC at 4141.448 kb on - strand, within frwC at 4141.449 kb on + strand, within frwC at 4141.450 kb on + strand, within frwC at 4141.494 kb on + strand, within frwC at 4141.549 kb on - strand at 4141.549 kb on - strand at 4141.676 kb on + strand at 4141.692 kb on - strand, within frwB at 4141.692 kb on - strand, within frwB at 4141.816 kb on + strand, within frwB at 4141.832 kb on + strand, within frwB at 4141.832 kb on + strand, within frwB at 4141.969 kb on + strand at 4142.009 kb on + strand at 4142.028 kb on + strand at 4142.092 kb on + strand at 4142.204 kb on - strand at 4142.223 kb on + strand at 4142.225 kb on + strand at 4142.282 kb on + strand, within pflD at 4142.282 kb on + strand, within pflD at 4142.357 kb on - strand, within pflD at 4142.357 kb on - strand, within pflD at 4142.370 kb on - strand, within pflD at 4142.370 kb on - strand, within pflD at 4142.410 kb on + strand, within pflD at 4142.461 kb on + strand, within pflD at 4142.461 kb on + strand, within pflD at 4142.476 kb on - strand, within pflD at 4142.476 kb on - strand, within pflD at 4142.497 kb on - strand, within pflD at 4142.497 kb on - strand, within pflD at 4142.499 kb on + strand, within pflD at 4142.528 kb on - strand, within pflD at 4142.602 kb on + strand, within pflD at 4142.672 kb on + strand, within pflD at 4142.680 kb on + strand, within pflD at 4142.683 kb on - strand, within pflD at 4142.683 kb on - strand, within pflD at 4142.688 kb on - strand, within pflD at 4142.690 kb on + strand, within pflD at 4142.690 kb on + strand, within pflD at 4142.698 kb on + strand, within pflD at 4142.705 kb on + strand, within pflD at 4142.736 kb on + strand, within pflD at 4142.753 kb on - strand, within pflD at 4142.765 kb on - strand, within pflD at 4142.781 kb on + strand, within pflD at 4142.834 kb on + strand, within pflD at 4142.834 kb on + strand, within pflD at 4142.837 kb on + strand, within pflD at 4142.862 kb on - strand, within pflD at 4142.862 kb on - strand, within pflD at 4142.865 kb on + strand, within pflD at 4142.870 kb on + strand, within pflD at 4142.873 kb on - strand, within pflD at 4142.905 kb on + strand, within pflD at 4142.905 kb on + strand, within pflD at 4142.905 kb on + strand, within pflD at 4142.934 kb on + strand, within pflD at 4142.942 kb on - strand, within pflD at 4142.944 kb on + strand, within pflD at 4142.944 kb on + strand, within pflD at 4142.989 kb on + strand, within pflD at 4142.994 kb on + strand, within pflD at 4142.994 kb on + strand, within pflD at 4142.994 kb on + strand, within pflD at 4142.994 kb on + strand, within pflD at 4143.035 kb on - strand, within pflD at 4143.053 kb on - strand, within pflD at 4143.070 kb on + strand, within pflD at 4143.126 kb on - strand, within pflD at 4143.220 kb on + strand, within pflD at 4143.220 kb on + strand, within pflD at 4143.258 kb on + strand, within pflD at 4143.264 kb on - strand, within pflD at 4143.270 kb on + strand, within pflD at 4143.270 kb on + strand, within pflD at 4143.434 kb on + strand, within pflD at 4143.436 kb on - strand, within pflD at 4143.445 kb on - strand, within pflD at 4143.445 kb on - strand, within pflD at 4143.446 kb on + strand, within pflD at 4143.718 kb on + strand, within pflD at 4143.718 kb on + strand, within pflD at 4143.776 kb on + strand, within pflD at 4143.784 kb on - strand, within pflD at 4143.802 kb on - strand, within pflD at 4143.802 kb on - strand, within pflD at 4143.853 kb on - strand, within pflD at 4143.862 kb on + strand, within pflD at 4143.862 kb on + strand, within pflD at 4143.870 kb on + strand, within pflD at 4143.870 kb on + strand, within pflD at 4143.872 kb on + strand, within pflD at 4143.872 kb on - strand, within pflD at 4143.872 kb on - strand, within pflD at 4143.883 kb on + strand, within pflD at 4143.895 kb on - strand, within pflD at 4144.012 kb on + strand, within pflD at 4144.012 kb on + strand, within pflD at 4144.019 kb on + strand, within pflD at 4144.020 kb on - strand, within pflD at 4144.021 kb on + strand, within pflD at 4144.036 kb on + strand, within pflD at 4144.039 kb on - strand, within pflD at 4144.066 kb on - strand, within pflD at 4144.079 kb on + strand, within pflD at 4144.084 kb on - strand, within pflD at 4144.086 kb on + strand at 4144.133 kb on - strand at 4144.135 kb on + strand at 4144.135 kb on + strand at 4144.162 kb on - strand at 4144.235 kb on + strand at 4144.235 kb on + strand at 4144.243 kb on - strand at 4144.243 kb on - strand at 4144.287 kb on - strand at 4144.304 kb on - strand at 4144.317 kb on - strand at 4144.317 kb on - strand at 4144.409 kb on + strand, within pflC at 4144.445 kb on - strand, within pflC at 4144.454 kb on + strand, within pflC at 4144.454 kb on + strand, within pflC at 4144.479 kb on + strand, within pflC at 4144.479 kb on + strand, within pflC at 4144.505 kb on - strand, within pflC at 4144.592 kb on - strand, within pflC at 4144.592 kb on - strand, within pflC at 4144.599 kb on + strand, within pflC at 4144.599 kb on + strand, within pflC at 4144.709 kb on + strand, within pflC at 4144.770 kb on - strand, within pflC at 4144.841 kb on + strand, within pflC at 4144.952 kb on + strand, within pflC at 4144.983 kb on - strand, within pflC at 4144.983 kb on - strand, within pflC at 4145.095 kb on - strand at 4145.097 kb on + strand at 4145.097 kb on + strand at 4145.109 kb on - strand at 4145.142 kb on - strand at 4145.142 kb on - strand at 4145.142 kb on - strand at 4145.190 kb on + strand at 4145.190 kb on + strand at 4145.261 kb on - strand, within frwD at 4145.263 kb on - strand, within frwD at 4145.264 kb on - strand, within frwD
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 4,141,051 - frwC b3949 0.46 +1.0 4,141,066 + frwC b3949 0.47 -0.7 4,141,069 + frwC b3949 0.48 +0.6 4,141,069 + frwC b3949 0.48 -2.3 4,141,069 + frwC b3949 0.48 +0.6 4,141,092 - frwC b3949 0.50 +1.0 4,141,105 + frwC b3949 0.51 -1.0 4,141,130 - frwC b3949 0.53 -0.7 4,141,133 + frwC b3949 0.54 -0.1 4,141,154 + frwC b3949 0.56 +0.7 4,141,167 + frwC b3949 0.57 -1.5 4,141,191 - frwC b3949 0.59 +0.5 4,141,194 - frwC b3949 0.59 +0.5 4,141,194 - frwC b3949 0.59 +0.1 4,141,218 + frwC b3949 0.62 +0.5 4,141,218 + frwC b3949 0.62 -0.3 4,141,255 + frwC b3949 0.65 -0.6 4,141,255 + frwC b3949 0.65 -0.1 4,141,311 + frwC b3949 0.70 -0.4 4,141,311 + frwC b3949 0.70 +0.3 4,141,320 - frwC b3949 0.71 +0.5 4,141,320 - frwC b3949 0.71 +1.6 4,141,320 - frwC b3949 0.71 +0.8 4,141,441 - frwC b3949 0.82 -0.0 4,141,448 - frwC b3949 0.83 -0.4 4,141,448 - frwC b3949 0.83 +0.1 4,141,449 + frwC b3949 0.83 +0.5 4,141,450 + frwC b3949 0.83 +0.4 4,141,494 + frwC b3949 0.87 +0.7 4,141,549 - -0.6 4,141,549 - +0.6 4,141,676 + +0.6 4,141,692 - frwB b3950 0.14 +0.5 4,141,692 - frwB b3950 0.14 +0.3 4,141,816 + frwB b3950 0.53 +0.2 4,141,832 + frwB b3950 0.58 -0.2 4,141,832 + frwB b3950 0.58 -0.1 4,141,969 + +0.9 4,142,009 + -0.7 4,142,028 + +0.1 4,142,092 + -0.1 4,142,204 - -1.4 4,142,223 + +0.3 4,142,225 + +0.5 4,142,282 + pflD b3951 0.11 +0.1 4,142,282 + pflD b3951 0.11 -0.8 4,142,357 - pflD b3951 0.15 -0.9 4,142,357 - pflD b3951 0.15 -0.4 4,142,370 - pflD b3951 0.15 -2.1 4,142,370 - pflD b3951 0.15 -1.6 4,142,410 + pflD b3951 0.17 +1.3 4,142,461 + pflD b3951 0.19 -0.2 4,142,461 + pflD b3951 0.19 -1.1 4,142,476 - pflD b3951 0.20 -1.1 4,142,476 - pflD b3951 0.20 -1.5 4,142,497 - pflD b3951 0.21 +0.7 4,142,497 - pflD b3951 0.21 +0.8 4,142,499 + pflD b3951 0.21 -0.5 4,142,528 - pflD b3951 0.22 +0.6 4,142,602 + pflD b3951 0.25 -1.3 4,142,672 + pflD b3951 0.28 +0.7 4,142,680 + pflD b3951 0.29 +0.5 4,142,683 - pflD b3951 0.29 +1.1 4,142,683 - pflD b3951 0.29 +0.0 4,142,688 - pflD b3951 0.29 -0.7 4,142,690 + pflD b3951 0.29 +1.0 4,142,690 + pflD b3951 0.29 -0.1 4,142,698 + pflD b3951 0.30 +0.0 4,142,705 + pflD b3951 0.30 -0.4 4,142,736 + pflD b3951 0.31 +1.5 4,142,753 - pflD b3951 0.32 -0.3 4,142,765 - pflD b3951 0.33 -1.1 4,142,781 + pflD b3951 0.33 +0.6 4,142,834 + pflD b3951 0.36 -1.7 4,142,834 + pflD b3951 0.36 -0.9 4,142,837 + pflD b3951 0.36 -1.3 4,142,862 - pflD b3951 0.37 +0.2 4,142,862 - pflD b3951 0.37 -0.2 4,142,865 + pflD b3951 0.37 -2.9 4,142,870 + pflD b3951 0.37 +0.7 4,142,873 - pflD b3951 0.37 -0.2 4,142,905 + pflD b3951 0.39 -0.8 4,142,905 + pflD b3951 0.39 -0.3 4,142,905 + pflD b3951 0.39 +0.2 4,142,934 + pflD b3951 0.40 +0.9 4,142,942 - pflD b3951 0.40 -0.5 4,142,944 + pflD b3951 0.40 -0.9 4,142,944 + pflD b3951 0.40 +0.8 4,142,989 + pflD b3951 0.42 -0.1 4,142,994 + pflD b3951 0.42 -0.0 4,142,994 + pflD b3951 0.42 -1.3 4,142,994 + pflD b3951 0.42 +0.6 4,142,994 + pflD b3951 0.42 +0.9 4,143,035 - pflD b3951 0.44 +0.2 4,143,053 - pflD b3951 0.45 -0.3 4,143,070 + pflD b3951 0.46 -0.4 4,143,126 - pflD b3951 0.48 +0.1 4,143,220 + pflD b3951 0.52 +0.5 4,143,220 + pflD b3951 0.52 +1.0 4,143,258 + pflD b3951 0.54 +0.4 4,143,264 - pflD b3951 0.54 -0.1 4,143,270 + pflD b3951 0.54 -1.3 4,143,270 + pflD b3951 0.54 -0.3 4,143,434 + pflD b3951 0.62 -0.6 4,143,436 - pflD b3951 0.62 -0.6 4,143,445 - pflD b3951 0.62 +0.1 4,143,445 - pflD b3951 0.62 -0.5 4,143,446 + pflD b3951 0.62 +0.1 4,143,718 + pflD b3951 0.74 +0.2 4,143,718 + pflD b3951 0.74 +0.3 4,143,776 + pflD b3951 0.77 +0.4 4,143,784 - pflD b3951 0.77 -0.4 4,143,802 - pflD b3951 0.78 -0.5 4,143,802 - pflD b3951 0.78 +0.4 4,143,853 - pflD b3951 0.80 +0.4 4,143,862 + pflD b3951 0.80 +0.1 4,143,862 + pflD b3951 0.80 -1.0 4,143,870 + pflD b3951 0.81 -0.1 4,143,870 + pflD b3951 0.81 +0.4 4,143,872 + pflD b3951 0.81 -0.9 4,143,872 - pflD b3951 0.81 -1.0 4,143,872 - pflD b3951 0.81 +0.6 4,143,883 + pflD b3951 0.81 +0.9 4,143,895 - pflD b3951 0.82 +0.9 4,144,012 + pflD b3951 0.87 -0.5 4,144,012 + pflD b3951 0.87 +0.3 4,144,019 + pflD b3951 0.87 +1.1 4,144,020 - pflD b3951 0.87 -1.2 4,144,021 + pflD b3951 0.87 -0.9 4,144,036 + pflD b3951 0.88 -0.1 4,144,039 - pflD b3951 0.88 -0.7 4,144,066 - pflD b3951 0.89 +0.5 4,144,079 + pflD b3951 0.90 -0.5 4,144,084 - pflD b3951 0.90 -0.7 4,144,086 + -0.3 4,144,133 - +0.5 4,144,135 + +0.4 4,144,135 + +0.1 4,144,162 - +0.8 4,144,235 + +1.5 4,144,235 + -0.1 4,144,243 - +0.9 4,144,243 - -0.2 4,144,287 - +0.7 4,144,304 - +0.5 4,144,317 - -0.3 4,144,317 - +0.2 4,144,409 + pflC b3952 0.15 -0.9 4,144,445 - pflC b3952 0.19 -0.2 4,144,454 + pflC b3952 0.20 +1.5 4,144,454 + pflC b3952 0.20 +0.7 4,144,479 + pflC b3952 0.23 +1.0 4,144,479 + pflC b3952 0.23 +0.3 4,144,505 - pflC b3952 0.25 -0.3 4,144,592 - pflC b3952 0.35 -1.3 4,144,592 - pflC b3952 0.35 -0.9 4,144,599 + pflC b3952 0.36 +0.1 4,144,599 + pflC b3952 0.36 +0.4 4,144,709 + pflC b3952 0.49 +0.2 4,144,770 - pflC b3952 0.56 -1.0 4,144,841 + pflC b3952 0.64 +0.2 4,144,952 + pflC b3952 0.76 -0.2 4,144,983 - pflC b3952 0.80 -0.1 4,144,983 - pflC b3952 0.80 +1.2 4,145,095 - +0.9 4,145,097 + -1.8 4,145,097 + -0.7 4,145,109 - -1.9 4,145,142 - -0.6 4,145,142 - +0.2 4,145,142 - -0.2 4,145,190 + +1.5 4,145,190 + +0.8 4,145,261 - frwD b3953 0.29 -0.0 4,145,263 - frwD b3953 0.30 -0.7 4,145,264 - frwD b3953 0.30 +0.2
Or see this region's nucleotide sequence