Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrE and rph are separated by 94 nucleotides rph and yicC are separated by 126 nucleotides yicC and dinD are separated by 220 nucleotides
b3642: pyrE - orotate phosphoribosyltransferase (NCBI), at 3,813,150 to 3,813,791
pyrE
b3643: rph - ribonuclease PH (NCBI), at 3,813,886 to 3,814,572
rph
b3644: yicC - hypothetical protein (NCBI), at 3,814,699 to 3,815,562
yicC
b3645: dinD - DNA-damage-inducible protein (VIMSS), at 3,815,783 to 3,816,607
dinD
Position (kb)
3814
3815
3816 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3813.743 kb on - strand at 3813.755 kb on + strand at 3813.764 kb on + strand at 3813.764 kb on + strand at 3813.769 kb on + strand at 3813.787 kb on - strand at 3813.793 kb on - strand at 3813.793 kb on - strand at 3813.834 kb on + strand at 3813.834 kb on + strand at 3813.845 kb on - strand at 3813.910 kb on + strand at 3813.910 kb on + strand at 3813.910 kb on + strand at 3813.910 kb on + strand at 3813.923 kb on + strand at 3813.923 kb on + strand at 3813.923 kb on - strand at 3813.923 kb on - strand at 3813.936 kb on + strand at 3813.936 kb on + strand at 3814.019 kb on + strand, within rph at 3814.041 kb on - strand, within rph at 3814.041 kb on - strand, within rph at 3814.094 kb on - strand, within rph at 3814.196 kb on - strand, within rph at 3814.199 kb on - strand, within rph at 3814.201 kb on - strand, within rph at 3814.201 kb on - strand, within rph at 3814.204 kb on + strand, within rph at 3814.204 kb on + strand, within rph at 3814.221 kb on + strand, within rph at 3814.248 kb on + strand, within rph at 3814.248 kb on + strand, within rph at 3814.256 kb on - strand, within rph at 3814.316 kb on + strand, within rph at 3814.322 kb on + strand, within rph at 3814.349 kb on - strand, within rph at 3814.349 kb on - strand, within rph at 3814.360 kb on - strand, within rph at 3814.370 kb on - strand, within rph at 3814.379 kb on + strand, within rph at 3814.379 kb on + strand, within rph at 3814.416 kb on - strand, within rph at 3814.448 kb on - strand, within rph at 3814.451 kb on - strand, within rph at 3814.451 kb on - strand, within rph at 3814.454 kb on + strand, within rph at 3814.462 kb on - strand, within rph at 3814.462 kb on - strand, within rph at 3814.483 kb on + strand, within rph at 3814.488 kb on + strand, within rph at 3814.488 kb on + strand, within rph at 3814.488 kb on + strand, within rph at 3814.535 kb on - strand at 3814.535 kb on - strand at 3814.535 kb on - strand at 3814.535 kb on - strand at 3814.565 kb on - strand at 3814.591 kb on + strand at 3814.591 kb on + strand at 3814.617 kb on + strand at 3814.639 kb on + strand at 3814.702 kb on - strand at 3814.717 kb on + strand at 3814.717 kb on - strand at 3814.717 kb on - strand at 3814.717 kb on - strand at 3814.717 kb on - strand at 3814.717 kb on - strand at 3814.725 kb on - strand at 3814.725 kb on - strand at 3814.783 kb on - strand at 3814.829 kb on + strand, within yicC at 3814.829 kb on + strand, within yicC at 3814.859 kb on + strand, within yicC at 3814.867 kb on - strand, within yicC at 3814.886 kb on + strand, within yicC at 3814.904 kb on - strand, within yicC at 3815.021 kb on + strand, within yicC at 3815.031 kb on - strand, within yicC at 3815.031 kb on - strand, within yicC at 3815.040 kb on + strand, within yicC at 3815.172 kb on - strand, within yicC at 3815.172 kb on - strand, within yicC at 3815.189 kb on + strand, within yicC at 3815.355 kb on - strand, within yicC at 3815.392 kb on - strand, within yicC at 3815.475 kb on - strand, within yicC at 3815.475 kb on - strand, within yicC at 3815.483 kb on - strand at 3815.602 kb on + strand at 3815.606 kb on + strand at 3815.616 kb on - strand at 3815.755 kb on + strand at 3815.772 kb on - strand at 3815.774 kb on + strand at 3815.835 kb on + strand at 3815.835 kb on + strand at 3815.848 kb on - strand at 3815.855 kb on - strand at 3815.855 kb on - strand at 3815.861 kb on - strand at 3815.870 kb on + strand, within dinD at 3815.878 kb on - strand, within dinD at 3815.898 kb on + strand, within dinD at 3815.898 kb on - strand, within dinD at 3815.906 kb on + strand, within dinD at 3815.914 kb on - strand, within dinD at 3815.927 kb on + strand, within dinD at 3815.930 kb on + strand, within dinD at 3815.940 kb on + strand, within dinD at 3815.954 kb on + strand, within dinD at 3816.009 kb on - strand, within dinD at 3816.009 kb on - strand, within dinD at 3816.012 kb on + strand, within dinD at 3816.116 kb on - strand, within dinD at 3816.116 kb on - strand, within dinD at 3816.116 kb on - strand, within dinD at 3816.137 kb on + strand, within dinD at 3816.170 kb on + strand, within dinD at 3816.170 kb on + strand, within dinD at 3816.233 kb on + strand, within dinD at 3816.241 kb on - strand, within dinD at 3816.249 kb on - strand, within dinD at 3816.253 kb on + strand, within dinD at 3816.255 kb on + strand, within dinD at 3816.258 kb on + strand, within dinD at 3816.291 kb on + strand, within dinD at 3816.309 kb on - strand, within dinD at 3816.309 kb on - strand, within dinD at 3816.317 kb on - strand, within dinD at 3816.381 kb on + strand, within dinD at 3816.386 kb on - strand, within dinD at 3816.386 kb on - strand, within dinD at 3816.389 kb on - strand, within dinD at 3816.389 kb on - strand, within dinD at 3816.469 kb on + strand, within dinD at 3816.486 kb on - strand, within dinD at 3816.495 kb on + strand, within dinD at 3816.503 kb on - strand, within dinD at 3816.503 kb on - strand, within dinD at 3816.507 kb on + strand, within dinD at 3816.507 kb on + strand, within dinD at 3816.531 kb on + strand at 3816.561 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 3,813,743 - -0.3 3,813,755 + +0.7 3,813,764 + -1.1 3,813,764 + -0.9 3,813,769 + -1.2 3,813,787 - +1.2 3,813,793 - -0.0 3,813,793 - +0.1 3,813,834 + +0.4 3,813,834 + -1.5 3,813,845 - +0.5 3,813,910 + +0.1 3,813,910 + -1.6 3,813,910 + +1.7 3,813,910 + -0.9 3,813,923 + +0.2 3,813,923 + -0.4 3,813,923 - -1.4 3,813,923 - +1.4 3,813,936 + +0.1 3,813,936 + -0.9 3,814,019 + rph b3643 0.19 +0.2 3,814,041 - rph b3643 0.23 -1.0 3,814,041 - rph b3643 0.23 -0.3 3,814,094 - rph b3643 0.30 -0.4 3,814,196 - rph b3643 0.45 -0.0 3,814,199 - rph b3643 0.46 +0.1 3,814,201 - rph b3643 0.46 +0.7 3,814,201 - rph b3643 0.46 +0.1 3,814,204 + rph b3643 0.46 -0.2 3,814,204 + rph b3643 0.46 -0.2 3,814,221 + rph b3643 0.49 +1.8 3,814,248 + rph b3643 0.53 +0.6 3,814,248 + rph b3643 0.53 +0.1 3,814,256 - rph b3643 0.54 +0.8 3,814,316 + rph b3643 0.63 -0.9 3,814,322 + rph b3643 0.63 -1.8 3,814,349 - rph b3643 0.67 +0.3 3,814,349 - rph b3643 0.67 +0.8 3,814,360 - rph b3643 0.69 +0.3 3,814,370 - rph b3643 0.70 -0.2 3,814,379 + rph b3643 0.72 -0.3 3,814,379 + rph b3643 0.72 -0.2 3,814,416 - rph b3643 0.77 -0.3 3,814,448 - rph b3643 0.82 -0.2 3,814,451 - rph b3643 0.82 -0.5 3,814,451 - rph b3643 0.82 -0.9 3,814,454 + rph b3643 0.83 +1.2 3,814,462 - rph b3643 0.84 +0.8 3,814,462 - rph b3643 0.84 -0.2 3,814,483 + rph b3643 0.87 -0.5 3,814,488 + rph b3643 0.88 -0.1 3,814,488 + rph b3643 0.88 +1.4 3,814,488 + rph b3643 0.88 -0.9 3,814,535 - -0.1 3,814,535 - -0.1 3,814,535 - -0.3 3,814,535 - -0.1 3,814,565 - -0.3 3,814,591 + -0.6 3,814,591 + +0.5 3,814,617 + +0.3 3,814,639 + +0.3 3,814,702 - -0.9 3,814,717 + -2.4 3,814,717 - -2.3 3,814,717 - +0.5 3,814,717 - -1.2 3,814,717 - -1.1 3,814,717 - +0.1 3,814,725 - -1.5 3,814,725 - +1.0 3,814,783 - -2.2 3,814,829 + yicC b3644 0.15 -0.0 3,814,829 + yicC b3644 0.15 -0.7 3,814,859 + yicC b3644 0.19 -1.0 3,814,867 - yicC b3644 0.19 -1.1 3,814,886 + yicC b3644 0.22 -0.0 3,814,904 - yicC b3644 0.24 -3.0 3,815,021 + yicC b3644 0.37 -1.5 3,815,031 - yicC b3644 0.38 -3.2 3,815,031 - yicC b3644 0.38 -1.6 3,815,040 + yicC b3644 0.39 -0.9 3,815,172 - yicC b3644 0.55 -1.3 3,815,172 - yicC b3644 0.55 -2.0 3,815,189 + yicC b3644 0.57 +0.5 3,815,355 - yicC b3644 0.76 +1.6 3,815,392 - yicC b3644 0.80 -1.6 3,815,475 - yicC b3644 0.90 -2.1 3,815,475 - yicC b3644 0.90 -0.7 3,815,483 - -1.3 3,815,602 + +0.5 3,815,606 + +1.3 3,815,616 - +0.0 3,815,755 + +0.0 3,815,772 - +0.1 3,815,774 + +0.2 3,815,835 + -0.2 3,815,835 + +0.7 3,815,848 - -0.0 3,815,855 - -0.3 3,815,855 - +0.6 3,815,861 - -0.7 3,815,870 + dinD b3645 0.11 -0.3 3,815,878 - dinD b3645 0.12 -1.0 3,815,898 + dinD b3645 0.14 +0.1 3,815,898 - dinD b3645 0.14 +0.4 3,815,906 + dinD b3645 0.15 -0.5 3,815,914 - dinD b3645 0.16 +0.1 3,815,927 + dinD b3645 0.17 -3.2 3,815,930 + dinD b3645 0.18 +0.6 3,815,940 + dinD b3645 0.19 -0.5 3,815,954 + dinD b3645 0.21 +0.3 3,816,009 - dinD b3645 0.27 +0.8 3,816,009 - dinD b3645 0.27 +0.2 3,816,012 + dinD b3645 0.28 +0.4 3,816,116 - dinD b3645 0.40 +0.3 3,816,116 - dinD b3645 0.40 +0.2 3,816,116 - dinD b3645 0.40 -1.6 3,816,137 + dinD b3645 0.43 -0.3 3,816,170 + dinD b3645 0.47 -1.7 3,816,170 + dinD b3645 0.47 -0.6 3,816,233 + dinD b3645 0.55 +0.1 3,816,241 - dinD b3645 0.56 -0.2 3,816,249 - dinD b3645 0.56 -0.2 3,816,253 + dinD b3645 0.57 -0.7 3,816,255 + dinD b3645 0.57 +0.6 3,816,258 + dinD b3645 0.58 +0.4 3,816,291 + dinD b3645 0.62 +0.1 3,816,309 - dinD b3645 0.64 +0.2 3,816,309 - dinD b3645 0.64 +0.7 3,816,317 - dinD b3645 0.65 -0.1 3,816,381 + dinD b3645 0.72 -0.2 3,816,386 - dinD b3645 0.73 +0.3 3,816,386 - dinD b3645 0.73 -0.1 3,816,389 - dinD b3645 0.73 -0.8 3,816,389 - dinD b3645 0.73 -0.5 3,816,469 + dinD b3645 0.83 +0.6 3,816,486 - dinD b3645 0.85 -0.2 3,816,495 + dinD b3645 0.86 +0.4 3,816,503 - dinD b3645 0.87 -0.2 3,816,503 - dinD b3645 0.87 +1.1 3,816,507 + dinD b3645 0.88 -0.3 3,816,507 + dinD b3645 0.88 -0.3 3,816,531 + -0.3 3,816,561 - +0.3
Or see this region's nucleotide sequence