Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yibK and cysE are separated by 52 nucleotides cysE and gpsA are separated by 79 nucleotides gpsA and secB overlap by 1 nucleotides secB and grxC are separated by 62 nucleotides grxC and yibN are separated by 141 nucleotides
b3606: yibK - predicted rRNA methylase (NCBI), at 3,779,238 to 3,779,711
yibK
b3607: cysE - serine acetyltransferase (NCBI), at 3,779,764 to 3,780,585
cysE
b3608: gpsA - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase (NCBI), at 3,780,665 to 3,781,684
gpsA
b3609: secB - export protein SecB (NCBI), at 3,781,684 to 3,782,151
secB
b3610: grxC - glutaredoxin 3 (NCBI), at 3,782,214 to 3,782,465
grxC
b3611: yibN - predicted rhodanese-related sulfurtransferase (NCBI), at 3,782,607 to 3,783,038
yibN
Position (kb)
3780
3781
3782 Strain fitness (log2 ratio)
-2
-1
0
1 at 3779.666 kb on + strand at 3779.668 kb on - strand at 3779.668 kb on - strand at 3779.704 kb on - strand at 3779.729 kb on + strand at 3779.739 kb on - strand at 3779.741 kb on - strand at 3780.590 kb on - strand at 3780.643 kb on - strand at 3780.643 kb on - strand at 3780.666 kb on - strand at 3781.687 kb on - strand at 3781.716 kb on - strand at 3781.744 kb on - strand, within secB at 3781.790 kb on - strand, within secB at 3781.862 kb on - strand, within secB at 3781.890 kb on - strand, within secB at 3781.930 kb on - strand, within secB at 3781.930 kb on - strand, within secB at 3781.967 kb on - strand, within secB at 3781.980 kb on - strand, within secB at 3781.990 kb on - strand, within secB at 3781.990 kb on - strand, within secB at 3782.150 kb on - strand at 3782.150 kb on - strand at 3782.159 kb on - strand at 3782.185 kb on - strand at 3782.192 kb on + strand at 3782.195 kb on - strand at 3782.200 kb on - strand at 3782.200 kb on - strand at 3782.200 kb on - strand at 3782.220 kb on - strand at 3782.241 kb on - strand, within grxC at 3782.260 kb on - strand, within grxC at 3782.264 kb on - strand, within grxC at 3782.264 kb on - strand, within grxC at 3782.275 kb on + strand, within grxC at 3782.277 kb on + strand, within grxC at 3782.277 kb on + strand, within grxC at 3782.280 kb on + strand, within grxC at 3782.280 kb on + strand, within grxC at 3782.283 kb on - strand, within grxC at 3782.308 kb on + strand, within grxC at 3782.308 kb on + strand, within grxC at 3782.314 kb on - strand, within grxC at 3782.337 kb on + strand, within grxC at 3782.366 kb on - strand, within grxC at 3782.366 kb on - strand, within grxC at 3782.381 kb on + strand, within grxC at 3782.381 kb on + strand, within grxC at 3782.381 kb on - strand, within grxC at 3782.395 kb on + strand, within grxC at 3782.416 kb on - strand, within grxC at 3782.433 kb on + strand, within grxC at 3782.527 kb on + strand at 3782.527 kb on + strand at 3782.529 kb on + strand at 3782.535 kb on - strand at 3782.545 kb on - strand at 3782.561 kb on + strand at 3782.578 kb on + strand at 3782.589 kb on - strand at 3782.646 kb on + strand at 3782.656 kb on + strand, within yibN
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 3,779,666 + +0.2 3,779,668 - -0.1 3,779,668 - +0.2 3,779,704 - -0.6 3,779,729 + -0.0 3,779,739 - +1.2 3,779,741 - +1.3 3,780,590 - -0.9 3,780,643 - -0.0 3,780,643 - -0.1 3,780,666 - -0.9 3,781,687 - -1.1 3,781,716 - +0.9 3,781,744 - secB b3609 0.13 -0.4 3,781,790 - secB b3609 0.23 -1.8 3,781,862 - secB b3609 0.38 -1.5 3,781,890 - secB b3609 0.44 -1.6 3,781,930 - secB b3609 0.53 +0.2 3,781,930 - secB b3609 0.53 -0.7 3,781,967 - secB b3609 0.60 +0.1 3,781,980 - secB b3609 0.63 +0.7 3,781,990 - secB b3609 0.65 -1.8 3,781,990 - secB b3609 0.65 -0.5 3,782,150 - +1.0 3,782,150 - +0.8 3,782,159 - +0.1 3,782,185 - +0.3 3,782,192 + -0.4 3,782,195 - -0.3 3,782,200 - -0.2 3,782,200 - +0.7 3,782,200 - +0.6 3,782,220 - -0.2 3,782,241 - grxC b3610 0.11 -0.3 3,782,260 - grxC b3610 0.18 -0.6 3,782,264 - grxC b3610 0.20 +0.8 3,782,264 - grxC b3610 0.20 -1.0 3,782,275 + grxC b3610 0.24 +1.1 3,782,277 + grxC b3610 0.25 +0.9 3,782,277 + grxC b3610 0.25 +0.7 3,782,280 + grxC b3610 0.26 +0.5 3,782,280 + grxC b3610 0.26 +0.8 3,782,283 - grxC b3610 0.27 -0.2 3,782,308 + grxC b3610 0.37 +0.2 3,782,308 + grxC b3610 0.37 +0.6 3,782,314 - grxC b3610 0.40 -1.4 3,782,337 + grxC b3610 0.49 -1.8 3,782,366 - grxC b3610 0.60 -0.3 3,782,366 - grxC b3610 0.60 -0.4 3,782,381 + grxC b3610 0.66 -0.4 3,782,381 + grxC b3610 0.66 +1.1 3,782,381 - grxC b3610 0.66 -0.5 3,782,395 + grxC b3610 0.72 -0.5 3,782,416 - grxC b3610 0.80 +1.2 3,782,433 + grxC b3610 0.87 +0.3 3,782,527 + -0.0 3,782,527 + +0.4 3,782,529 + +0.5 3,782,535 - -0.6 3,782,545 - -0.2 3,782,561 + +0.2 3,782,578 + +0.0 3,782,589 - +0.1 3,782,646 + +1.1 3,782,656 + yibN b3611 0.11 +1.2
Or see this region's nucleotide sequence