Strain Fitness in Escherichia coli BW25113 around b3608

Experiment: LB Anaerobic with Spiramycin 0.000125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyibK and cysE are separated by 52 nucleotidescysE and gpsA are separated by 79 nucleotidesgpsA and secB overlap by 1 nucleotidessecB and grxC are separated by 62 nucleotidesgrxC and yibN are separated by 141 nucleotides b3606: yibK - predicted rRNA methylase (NCBI), at 3,779,238 to 3,779,711 yibK b3607: cysE - serine acetyltransferase (NCBI), at 3,779,764 to 3,780,585 cysE b3608: gpsA - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase (NCBI), at 3,780,665 to 3,781,684 gpsA b3609: secB - export protein SecB (NCBI), at 3,781,684 to 3,782,151 secB b3610: grxC - glutaredoxin 3 (NCBI), at 3,782,214 to 3,782,465 grxC b3611: yibN - predicted rhodanese-related sulfurtransferase (NCBI), at 3,782,607 to 3,783,038 yibN Position (kb) 3780 3781 3782Strain fitness (log2 ratio) -2 -1 0 1at 3779.666 kb on + strandat 3779.668 kb on - strandat 3779.668 kb on - strandat 3779.704 kb on - strandat 3779.729 kb on + strandat 3779.739 kb on - strandat 3779.741 kb on - strandat 3780.590 kb on - strandat 3780.643 kb on - strandat 3780.643 kb on - strandat 3780.666 kb on - strandat 3781.687 kb on - strandat 3781.716 kb on - strandat 3781.744 kb on - strand, within secBat 3781.790 kb on - strand, within secBat 3781.862 kb on - strand, within secBat 3781.890 kb on - strand, within secBat 3781.930 kb on - strand, within secBat 3781.930 kb on - strand, within secBat 3781.967 kb on - strand, within secBat 3781.980 kb on - strand, within secBat 3781.990 kb on - strand, within secBat 3781.990 kb on - strand, within secBat 3782.150 kb on - strandat 3782.150 kb on - strandat 3782.159 kb on - strandat 3782.185 kb on - strandat 3782.192 kb on + strandat 3782.195 kb on - strandat 3782.200 kb on - strandat 3782.200 kb on - strandat 3782.200 kb on - strandat 3782.220 kb on - strandat 3782.241 kb on - strand, within grxCat 3782.260 kb on - strand, within grxCat 3782.264 kb on - strand, within grxCat 3782.264 kb on - strand, within grxCat 3782.275 kb on + strand, within grxCat 3782.277 kb on + strand, within grxCat 3782.277 kb on + strand, within grxCat 3782.280 kb on + strand, within grxCat 3782.280 kb on + strand, within grxCat 3782.283 kb on - strand, within grxCat 3782.308 kb on + strand, within grxCat 3782.308 kb on + strand, within grxCat 3782.314 kb on - strand, within grxCat 3782.337 kb on + strand, within grxCat 3782.366 kb on - strand, within grxCat 3782.366 kb on - strand, within grxCat 3782.381 kb on + strand, within grxCat 3782.381 kb on + strand, within grxCat 3782.381 kb on - strand, within grxCat 3782.395 kb on + strand, within grxCat 3782.416 kb on - strand, within grxCat 3782.433 kb on + strand, within grxCat 3782.527 kb on + strandat 3782.527 kb on + strandat 3782.529 kb on + strandat 3782.535 kb on - strandat 3782.545 kb on - strandat 3782.561 kb on + strandat 3782.578 kb on + strandat 3782.589 kb on - strandat 3782.646 kb on + strandat 3782.656 kb on + strand, within yibN

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM
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3,779,666 + +0.2
3,779,668 - -0.1
3,779,668 - +0.2
3,779,704 - -0.6
3,779,729 + -0.0
3,779,739 - +1.2
3,779,741 - +1.3
3,780,590 - -0.9
3,780,643 - -0.0
3,780,643 - -0.1
3,780,666 - -0.9
3,781,687 - -1.1
3,781,716 - +0.9
3,781,744 - secB b3609 0.13 -0.4
3,781,790 - secB b3609 0.23 -1.8
3,781,862 - secB b3609 0.38 -1.5
3,781,890 - secB b3609 0.44 -1.6
3,781,930 - secB b3609 0.53 +0.2
3,781,930 - secB b3609 0.53 -0.7
3,781,967 - secB b3609 0.60 +0.1
3,781,980 - secB b3609 0.63 +0.7
3,781,990 - secB b3609 0.65 -1.8
3,781,990 - secB b3609 0.65 -0.5
3,782,150 - +1.0
3,782,150 - +0.8
3,782,159 - +0.1
3,782,185 - +0.3
3,782,192 + -0.4
3,782,195 - -0.3
3,782,200 - -0.2
3,782,200 - +0.7
3,782,200 - +0.6
3,782,220 - -0.2
3,782,241 - grxC b3610 0.11 -0.3
3,782,260 - grxC b3610 0.18 -0.6
3,782,264 - grxC b3610 0.20 +0.8
3,782,264 - grxC b3610 0.20 -1.0
3,782,275 + grxC b3610 0.24 +1.1
3,782,277 + grxC b3610 0.25 +0.9
3,782,277 + grxC b3610 0.25 +0.7
3,782,280 + grxC b3610 0.26 +0.5
3,782,280 + grxC b3610 0.26 +0.8
3,782,283 - grxC b3610 0.27 -0.2
3,782,308 + grxC b3610 0.37 +0.2
3,782,308 + grxC b3610 0.37 +0.6
3,782,314 - grxC b3610 0.40 -1.4
3,782,337 + grxC b3610 0.49 -1.8
3,782,366 - grxC b3610 0.60 -0.3
3,782,366 - grxC b3610 0.60 -0.4
3,782,381 + grxC b3610 0.66 -0.4
3,782,381 + grxC b3610 0.66 +1.1
3,782,381 - grxC b3610 0.66 -0.5
3,782,395 + grxC b3610 0.72 -0.5
3,782,416 - grxC b3610 0.80 +1.2
3,782,433 + grxC b3610 0.87 +0.3
3,782,527 + -0.0
3,782,527 + +0.4
3,782,529 + +0.5
3,782,535 - -0.6
3,782,545 - -0.2
3,782,561 + +0.2
3,782,578 + +0.0
3,782,589 - +0.1
3,782,646 + +1.1
3,782,656 + yibN b3611 0.11 +1.2

Or see this region's nucleotide sequence