Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dcuD and sspB are separated by 42 nucleotides sspB and sspA are separated by 5 nucleotides sspA and rpsI are separated by 394 nucleotides rpsI and rplM are separated by 15 nucleotides
b3227: dcuD - predicted transporter (NCBI), at 3,372,891 to 3,374,258
dcuD
b3228: sspB - ClpXP protease specificity-enhancing factor (NCBI), at 3,374,301 to 3,374,798
sspB
b3229: sspA - stringent starvation protein A (NCBI), at 3,374,804 to 3,375,442
sspA
b3230: rpsI - 30S ribosomal protein S9 (NCBI), at 3,375,837 to 3,376,229
rpsI
b3231: rplM - 50S ribosomal protein L13 (NCBI), at 3,376,245 to 3,376,673
rplM
Position (kb)
3374
3375
3376 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3373.896 kb on + strand, within dcuD at 3373.897 kb on - strand, within dcuD at 3373.924 kb on + strand, within dcuD at 3373.924 kb on + strand, within dcuD at 3373.932 kb on - strand, within dcuD at 3373.932 kb on - strand, within dcuD at 3373.933 kb on - strand at 3373.933 kb on - strand, within dcuD at 3373.984 kb on + strand, within dcuD at 3374.004 kb on - strand, within dcuD at 3374.052 kb on + strand, within dcuD at 3374.060 kb on - strand, within dcuD at 3374.103 kb on + strand, within dcuD at 3374.128 kb on + strand at 3374.209 kb on - strand at 3374.249 kb on + strand at 3374.249 kb on + strand at 3374.306 kb on - strand at 3374.306 kb on - strand at 3374.366 kb on - strand, within sspB at 3374.472 kb on - strand, within sspB at 3374.472 kb on - strand, within sspB at 3374.484 kb on - strand, within sspB at 3374.484 kb on - strand, within sspB at 3374.514 kb on + strand, within sspB at 3374.543 kb on - strand, within sspB at 3374.543 kb on - strand, within sspB at 3374.563 kb on + strand, within sspB at 3374.667 kb on + strand, within sspB at 3374.667 kb on + strand, within sspB at 3374.675 kb on - strand, within sspB at 3374.675 kb on - strand, within sspB at 3374.684 kb on + strand, within sspB at 3374.684 kb on + strand, within sspB at 3374.966 kb on - strand, within sspA at 3374.976 kb on + strand, within sspA at 3374.976 kb on + strand, within sspA at 3375.071 kb on - strand, within sspA at 3375.073 kb on - strand, within sspA at 3375.084 kb on - strand, within sspA at 3375.098 kb on + strand, within sspA at 3375.098 kb on + strand, within sspA at 3375.113 kb on - strand, within sspA at 3375.189 kb on - strand, within sspA at 3375.220 kb on - strand, within sspA at 3375.220 kb on - strand, within sspA at 3375.220 kb on - strand, within sspA at 3375.236 kb on - strand, within sspA at 3375.353 kb on + strand, within sspA at 3375.353 kb on + strand, within sspA at 3375.515 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 3,373,896 + dcuD b3227 0.73 +0.1 3,373,897 - dcuD b3227 0.74 -0.0 3,373,924 + dcuD b3227 0.76 +0.7 3,373,924 + dcuD b3227 0.76 +0.8 3,373,932 - dcuD b3227 0.76 -0.7 3,373,932 - dcuD b3227 0.76 -1.1 3,373,933 - +0.5 3,373,933 - dcuD b3227 0.76 +1.1 3,373,984 + dcuD b3227 0.80 -0.2 3,374,004 - dcuD b3227 0.81 +1.5 3,374,052 + dcuD b3227 0.85 -0.2 3,374,060 - dcuD b3227 0.85 +0.3 3,374,103 + dcuD b3227 0.89 -0.9 3,374,128 + -0.9 3,374,209 - -0.1 3,374,249 + -2.4 3,374,249 + +0.5 3,374,306 - +0.7 3,374,306 - +0.9 3,374,366 - sspB b3228 0.13 +0.8 3,374,472 - sspB b3228 0.34 +0.5 3,374,472 - sspB b3228 0.34 +1.5 3,374,484 - sspB b3228 0.37 +0.7 3,374,484 - sspB b3228 0.37 +1.4 3,374,514 + sspB b3228 0.43 +0.1 3,374,543 - sspB b3228 0.49 -0.0 3,374,543 - sspB b3228 0.49 +0.3 3,374,563 + sspB b3228 0.53 -0.9 3,374,667 + sspB b3228 0.73 +0.5 3,374,667 + sspB b3228 0.73 -1.2 3,374,675 - sspB b3228 0.75 -0.5 3,374,675 - sspB b3228 0.75 -0.7 3,374,684 + sspB b3228 0.77 -0.1 3,374,684 + sspB b3228 0.77 +2.7 3,374,966 - sspA b3229 0.25 +0.8 3,374,976 + sspA b3229 0.27 +0.5 3,374,976 + sspA b3229 0.27 +0.1 3,375,071 - sspA b3229 0.42 -1.6 3,375,073 - sspA b3229 0.42 +1.5 3,375,084 - sspA b3229 0.44 -1.4 3,375,098 + sspA b3229 0.46 +1.1 3,375,098 + sspA b3229 0.46 +0.5 3,375,113 - sspA b3229 0.48 -0.5 3,375,189 - sspA b3229 0.60 -1.2 3,375,220 - sspA b3229 0.65 +1.1 3,375,220 - sspA b3229 0.65 +0.5 3,375,220 - sspA b3229 0.65 -0.5 3,375,236 - sspA b3229 0.68 -0.2 3,375,353 + sspA b3229 0.86 +2.1 3,375,353 + sspA b3229 0.86 +0.5 3,375,515 - -0.6
Or see this region's nucleotide sequence