Strain Fitness in Escherichia coli BW25113 around b2866

Experiment: LB Anaerobic with Spiramycin 0.000125 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Anaerobic with Spiramycin 0.000125 mM
2,997,372	-	b2865	b2865	0.28	-0.9
2,997,379	+	b2865	b2865	0.29	+0.1
2,997,379	+	b2865	b2865	0.29	+0.1
2,997,413	-	b2865	b2865	0.34	+1.1
2,997,413	-	b2865	b2865	0.34	+0.1
2,997,429	-	b2865	b2865	0.36	-0.1
2,997,429	-	b2865	b2865	0.36	-0.6
2,997,435	+	b2865	b2865	0.37	-1.2
2,997,437	+	b2865	b2865	0.37	-1.5
2,997,444	+	b2865	b2865	0.38	-1.0
2,997,450	+	b2865	b2865	0.39	+0.7
2,997,450	+	b2865	b2865	0.39	-0.1
2,997,452	-	b2865	b2865	0.39	+0.2
2,997,525	+	b2865	b2865	0.49	-0.1
2,997,537	-	b2865	b2865	0.50	-0.3
2,997,553	-	b2865	b2865	0.52	-1.1
2,997,587	-	b2865	b2865	0.57	+0.7
2,997,602	+	b2865	b2865	0.59	+0.8
2,997,632	+	b2865	b2865	0.63	-0.4
2,997,696	+	b2865	b2865	0.71	+0.3
2,997,698	-	b2865	b2865	0.71	-0.5
2,997,700	+	b2865	b2865	0.72	+1.9
2,997,727	+	b2865	b2865	0.75	-0.2
2,997,727	+	b2865	b2865	0.75	-0.3
2,997,738	+	b2865	b2865	0.77	-1.4
2,997,754	+	b2865	b2865	0.79	+1.5
2,997,759	+	b2865	b2865	0.79	+0.3
2,997,759	+	b2865	b2865	0.79	+1.3
2,997,761	+	b2865	b2865	0.80	+0.1
2,997,789	-	b2865	b2865	0.83	-0.4
2,997,794	-	b2865	b2865	0.84	-0.9
2,997,795	-	b2865	b2865	0.84	-0.1
2,997,796	-	b2865	b2865	0.84	-0.9
2,997,830	-	b2865	b2865	0.89	-1.7
2,997,866	+				+0.2
2,997,906	-				-1.9
2,997,906	-				+1.5
2,997,946	+				-0.6
2,997,950	-				-0.6
2,997,953	-				+1.9
2,997,960	-				+0.7
2,997,963	+				+0.3
2,997,967	-				-0.2
2,997,967	-				-1.2
2,997,967	-				+0.3
2,997,975	-				-0.8
2,997,978	-				-0.7
2,998,066	-				-0.2
2,998,066	-				-0.7
2,998,108	-				+0.1
2,998,158	-				+0.1
2,998,159	-				-0.3
2,998,159	-				-1.0
2,998,166	+				+1.9
2,998,224	+				-0.3
2,998,224	+				-0.7
2,998,225	-				-0.8
2,998,234	+				+5.2
2,998,242	-				-0.1
2,998,242	-				-0.8
2,998,353	-				+1.9
2,998,363	+				+0.4
2,998,363	+				+0.1
2,998,383	+				+0.2
2,998,388	-				+0.0
2,998,414	-				-0.7
2,998,416	-				-1.6
2,998,416	-				+0.7
2,998,432	+				-2.9
2,998,441	-				-0.1
2,998,451	-				+0.4
2,998,482	-				+0.6
2,998,482	-				-1.4
2,998,488	+				-0.0
2,998,573	+				-0.3
2,998,573	+				-0.5
2,998,575	-				+0.7
2,998,575	-				-0.5
2,998,581	-				-0.5
2,998,581	-				+1.3
2,998,755	+	xdhA	b2866	0.17	-0.4
2,998,828	+	xdhA	b2866	0.20	+0.2
2,998,867	+	xdhA	b2866	0.22	-1.3
2,998,874	-	xdhA	b2866	0.22	-0.6
2,998,891	+	xdhA	b2866	0.23	-1.0
2,998,936	+	xdhA	b2866	0.25	-0.9
2,999,159	-	xdhA	b2866	0.35	+0.3
2,999,200	+	xdhA	b2866	0.37	-1.1
2,999,275	-	xdhA	b2866	0.40	+0.0
2,999,313	+	xdhA	b2866	0.42	+1.2
2,999,321	-	xdhA	b2866	0.42	+0.8
2,999,380	+	xdhA	b2866	0.45	+0.7
2,999,380	+	xdhA	b2866	0.45	+0.8
2,999,452	+	xdhA	b2866	0.48	+1.1
2,999,452	+	xdhA	b2866	0.48	+0.0
2,999,460	-	xdhA	b2866	0.48	-0.0
2,999,565	+	xdhA	b2866	0.53	+0.0
2,999,573	-	xdhA	b2866	0.53	+0.8
2,999,700	-	xdhA	b2866	0.59	+0.2
2,999,765	+	xdhA	b2866	0.62	+0.2
2,999,878	-	xdhA	b2866	0.67	+1.3
2,999,898	+	xdhA	b2866	0.68	-1.2
2,999,898	+	xdhA	b2866	0.68	-0.7
2,999,939	+	xdhA	b2866	0.70	-2.5
2,999,950	-	xdhA	b2866	0.70	+0.7
2,999,950	-	xdhA	b2866	0.70	+0.0
3,000,004	+	xdhA	b2866	0.72	-0.2
3,000,004	+	xdhA	b2866	0.72	+0.2
3,000,080	-	xdhA	b2866	0.76	-0.3
3,000,115	-	xdhA	b2866	0.77	-0.4
3,000,115	-	xdhA	b2866	0.77	+0.3
3,000,119	+	xdhA	b2866	0.78	+0.1
3,000,128	+	xdhA	b2866	0.78	+0.6
3,000,136	-	xdhA	b2866	0.78	+1.2
3,000,228	-	xdhA	b2866	0.82	+1.0
3,000,270	+	xdhA	b2866	0.84	-0.1
3,000,384	-	xdhA	b2866	0.89	-0.1
3,000,421	-				+0.1
3,000,526	+				-2.8
3,000,526	+				-0.4
3,000,536	+				-0.7
3,000,561	+				+0.1
3,000,604	+				+0.5
3,000,656	-				+0.7
3,000,664	+				+0.8
3,000,669	+				-0.6
3,000,730	+	xdhB	b2867	0.11	-0.8
3,000,747	-	xdhB	b2867	0.13	+0.5
3,000,862	+	xdhB	b2867	0.26	-0.2
3,000,862	+	xdhB	b2867	0.26	+0.1
3,000,862	+	xdhB	b2867	0.26	-0.4
3,000,896	+	xdhB	b2867	0.30	+1.3
3,001,048	+	xdhB	b2867	0.47	-0.2
3,001,048	+	xdhB	b2867	0.47	+1.3
3,001,229	-	xdhB	b2867	0.67	-1.4
3,001,290	-	xdhB	b2867	0.74	+0.4
3,001,329	-	xdhB	b2867	0.79	+0.4
3,001,329	-	xdhB	b2867	0.79	-0.9
3,001,468	-				+0.5
3,001,567	+	xdhC	b2868	0.12	-0.3
3,001,567	+	xdhC	b2868	0.12	+1.7
3,001,616	+	xdhC	b2868	0.22	+0.3
3,001,616	+	xdhC	b2868	0.22	+1.5