Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygeK and ygeN are separated by 221 nucleotides ygeN and ygeO overlap by 59 nucleotides ygeO and insD-4 are separated by 11 nucleotides insD-4 and insC overlap by 43 nucleotides
b2856: ygeK - hypothetical protein (RefSeq), at 2,992,482 to 2,993,114
ygeK
b2858: ygeN - hypothetical protein (NCBI), at 2,993,336 to 2,994,042
ygeN
b2859: ygeO - hypothetical protein (RefSeq), at 2,993,984 to 2,994,382
ygeO
b2860: insD-4 - IS2 insertion element transposase InsAB' (NCBI), at 2,994,394 to 2,995,299
insD-4
b2861: insC - KpLE2 phage-like element; IS2 insertion element repressor InsA (RefSeq), at 2,995,257 to 2,995,622
insC
Position (kb)
2993
2994
2995 Strain fitness (log2 ratio)
-1
0
1
2 at 2994.162 kb on - strand, within ygeO at 2994.221 kb on - strand, within ygeO at 2994.286 kb on - strand, within ygeO at 2994.297 kb on - strand, within ygeO at 2994.318 kb on - strand, within ygeO at 2994.341 kb on - strand, within ygeO at 2994.407 kb on - strand at 2994.407 kb on - strand at 2994.419 kb on - strand at 2994.419 kb on - strand at 2994.420 kb on - strand at 2994.435 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 2,994,162 - ygeO b2859 0.45 +1.9 2,994,221 - ygeO b2859 0.59 +1.9 2,994,286 - ygeO b2859 0.76 +0.3 2,994,297 - ygeO b2859 0.78 +0.3 2,994,318 - ygeO b2859 0.84 -0.9 2,994,341 - ygeO b2859 0.89 -1.2 2,994,407 - +1.2 2,994,407 - +0.1 2,994,419 - -0.1 2,994,419 - +0.9 2,994,420 - +0.0 2,994,435 - +0.2
Or see this region's nucleotide sequence