Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hycI and hycH overlap by 8 nucleotides hycH and hycG overlap by 4 nucleotides hycG and hycF overlap by 1 nucleotides hycF and hycE are separated by 9 nucleotides
b2717: hycI - protease involved in processing C-terminal end of HycE (NCBI), at 2,840,595 to 2,841,065
hycI
b2718: hycH - protein required for maturation of hydrogenase 3 (NCBI), at 2,841,058 to 2,841,468
hycH
b2719: hycG - hydrogenase 3 and formate hydrogenase complex, HycG subunit (NCBI), at 2,841,465 to 2,842,232
hycG
b2720: hycF - formate hydrogenlyase complex iron-sulfur protein (NCBI), at 2,842,232 to 2,842,774
hycF
b2721: hycE - hydrogenase 3, large subunit (NCBI), at 2,842,784 to 2,844,493
hycE
Position (kb)
2841
2842
2843 Strain fitness (log2 ratio)
-1
0
1
2 at 2840.497 kb on - strand at 2840.551 kb on - strand at 2840.558 kb on + strand at 2840.644 kb on + strand, within hycI at 2840.688 kb on - strand, within hycI at 2840.699 kb on + strand, within hycI at 2840.699 kb on + strand, within hycI at 2840.711 kb on - strand, within hycI at 2840.711 kb on - strand, within hycI at 2840.731 kb on - strand, within hycI at 2840.731 kb on - strand, within hycI at 2841.008 kb on + strand, within hycI at 2841.071 kb on + strand at 2841.119 kb on + strand, within hycH at 2841.150 kb on + strand, within hycH at 2841.167 kb on + strand, within hycH at 2841.175 kb on - strand, within hycH at 2841.207 kb on + strand, within hycH at 2841.225 kb on + strand, within hycH at 2841.272 kb on - strand, within hycH at 2841.300 kb on + strand, within hycH at 2841.300 kb on + strand, within hycH at 2841.327 kb on - strand, within hycH at 2841.327 kb on - strand, within hycH at 2841.338 kb on - strand, within hycH at 2841.384 kb on - strand, within hycH at 2841.572 kb on + strand, within hycG at 2841.581 kb on - strand, within hycG at 2841.622 kb on - strand, within hycG at 2841.645 kb on - strand, within hycG at 2841.648 kb on - strand, within hycG at 2841.713 kb on + strand, within hycG at 2841.774 kb on - strand, within hycG at 2841.821 kb on - strand, within hycG at 2841.861 kb on - strand, within hycG at 2841.870 kb on - strand, within hycG at 2842.019 kb on + strand, within hycG at 2842.047 kb on - strand, within hycG at 2842.093 kb on - strand, within hycG at 2842.093 kb on - strand, within hycG at 2842.113 kb on + strand, within hycG at 2842.245 kb on + strand at 2842.253 kb on - strand at 2842.346 kb on + strand, within hycF at 2842.367 kb on - strand, within hycF at 2842.377 kb on + strand, within hycF at 2842.384 kb on - strand, within hycF at 2842.490 kb on + strand, within hycF at 2842.523 kb on - strand, within hycF at 2842.523 kb on - strand, within hycF at 2842.545 kb on + strand, within hycF at 2842.584 kb on + strand, within hycF at 2842.584 kb on + strand, within hycF at 2842.584 kb on + strand, within hycF at 2842.599 kb on + strand, within hycF at 2842.773 kb on - strand at 2842.853 kb on - strand at 2842.890 kb on + strand at 2842.890 kb on + strand at 2842.890 kb on + strand at 2843.106 kb on - strand, within hycE at 2843.139 kb on + strand, within hycE at 2843.144 kb on + strand, within hycE at 2843.147 kb on - strand, within hycE
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 2,840,497 - +0.2 2,840,551 - +0.5 2,840,558 + -0.4 2,840,644 + hycI b2717 0.10 +0.8 2,840,688 - hycI b2717 0.20 -1.5 2,840,699 + hycI b2717 0.22 -0.0 2,840,699 + hycI b2717 0.22 +0.8 2,840,711 - hycI b2717 0.25 +1.8 2,840,711 - hycI b2717 0.25 -0.2 2,840,731 - hycI b2717 0.29 -0.1 2,840,731 - hycI b2717 0.29 +0.8 2,841,008 + hycI b2717 0.88 -0.8 2,841,071 + +0.9 2,841,119 + hycH b2718 0.15 -0.4 2,841,150 + hycH b2718 0.22 +0.4 2,841,167 + hycH b2718 0.27 +0.5 2,841,175 - hycH b2718 0.28 +0.5 2,841,207 + hycH b2718 0.36 -0.6 2,841,225 + hycH b2718 0.41 -0.7 2,841,272 - hycH b2718 0.52 +0.1 2,841,300 + hycH b2718 0.59 -0.2 2,841,300 + hycH b2718 0.59 +0.2 2,841,327 - hycH b2718 0.65 +1.0 2,841,327 - hycH b2718 0.65 +0.5 2,841,338 - hycH b2718 0.68 -0.2 2,841,384 - hycH b2718 0.79 +0.5 2,841,572 + hycG b2719 0.14 +0.0 2,841,581 - hycG b2719 0.15 -0.1 2,841,622 - hycG b2719 0.20 +1.3 2,841,645 - hycG b2719 0.23 +0.7 2,841,648 - hycG b2719 0.24 -0.1 2,841,713 + hycG b2719 0.32 -0.5 2,841,774 - hycG b2719 0.40 -0.2 2,841,821 - hycG b2719 0.46 -0.3 2,841,861 - hycG b2719 0.52 +0.3 2,841,870 - hycG b2719 0.53 -0.8 2,842,019 + hycG b2719 0.72 +1.3 2,842,047 - hycG b2719 0.76 +0.8 2,842,093 - hycG b2719 0.82 +1.1 2,842,093 - hycG b2719 0.82 +1.0 2,842,113 + hycG b2719 0.84 -1.2 2,842,245 + -0.3 2,842,253 - -0.5 2,842,346 + hycF b2720 0.21 -0.3 2,842,367 - hycF b2720 0.25 -0.2 2,842,377 + hycF b2720 0.27 -0.6 2,842,384 - hycF b2720 0.28 +0.0 2,842,490 + hycF b2720 0.48 +0.2 2,842,523 - hycF b2720 0.54 -1.4 2,842,523 - hycF b2720 0.54 -0.3 2,842,545 + hycF b2720 0.58 +0.4 2,842,584 + hycF b2720 0.65 -0.9 2,842,584 + hycF b2720 0.65 -0.7 2,842,584 + hycF b2720 0.65 +0.1 2,842,599 + hycF b2720 0.68 +0.2 2,842,773 - +0.6 2,842,853 - +0.3 2,842,890 + -0.0 2,842,890 + -1.0 2,842,890 + +0.6 2,843,106 - hycE b2721 0.19 +0.1 2,843,139 + hycE b2721 0.21 +0.7 2,843,144 + hycE b2721 0.21 -0.4 2,843,147 - hycE b2721 0.21 -0.1
Or see this region's nucleotide sequence