Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nadB and yfiC overlap by 16 nucleotides yfiC and srmB are separated by 131 nucleotides srmB and yfiE are separated by 208 nucleotides
b2574: nadB - L-aspartate oxidase (NCBI), at 2,708,442 to 2,710,064
nadB
b2575: yfiC - putative enzyme (VIMSS), at 2,710,049 to 2,710,786
yfiC
b2576: srmB - ATP-dependent RNA helicase (NCBI), at 2,710,918 to 2,712,252
srmB
b2577: yfiE - putative transcriptional regulator LYSR-type (VIMSS), at 2,712,461 to 2,713,342
yfiE
Position (kb)
2710
2711
2712
2713 Strain fitness (log2 ratio)
-1
0
1
2 at 2709.919 kb on + strand at 2709.942 kb on - strand at 2709.942 kb on - strand at 2709.949 kb on + strand at 2709.954 kb on + strand at 2709.957 kb on - strand at 2709.997 kb on + strand at 2710.005 kb on - strand at 2710.005 kb on - strand at 2710.005 kb on - strand at 2710.189 kb on - strand, within yfiC at 2710.236 kb on + strand, within yfiC at 2710.329 kb on + strand, within yfiC at 2710.375 kb on - strand, within yfiC at 2710.398 kb on + strand, within yfiC at 2710.399 kb on - strand, within yfiC at 2710.399 kb on - strand, within yfiC at 2710.402 kb on - strand, within yfiC at 2710.409 kb on + strand, within yfiC at 2710.410 kb on + strand, within yfiC at 2710.413 kb on - strand, within yfiC at 2710.413 kb on - strand, within yfiC at 2710.418 kb on - strand, within yfiC at 2710.418 kb on - strand, within yfiC at 2710.439 kb on - strand, within yfiC at 2710.439 kb on - strand, within yfiC at 2710.591 kb on - strand, within yfiC at 2710.770 kb on + strand at 2710.770 kb on + strand at 2710.770 kb on + strand at 2710.770 kb on + strand at 2710.778 kb on - strand at 2710.778 kb on - strand at 2710.833 kb on - strand at 2710.833 kb on - strand at 2710.838 kb on + strand at 2711.086 kb on + strand, within srmB at 2711.159 kb on + strand, within srmB at 2711.181 kb on + strand, within srmB at 2711.268 kb on - strand, within srmB at 2711.305 kb on + strand, within srmB at 2711.376 kb on - strand, within srmB at 2711.425 kb on - strand, within srmB at 2711.557 kb on - strand, within srmB at 2711.666 kb on + strand, within srmB at 2711.698 kb on + strand, within srmB at 2711.747 kb on + strand at 2711.849 kb on + strand, within srmB at 2711.857 kb on - strand, within srmB at 2711.857 kb on - strand, within srmB at 2711.927 kb on + strand, within srmB at 2712.112 kb on - strand, within srmB at 2712.203 kb on + strand at 2712.218 kb on + strand at 2712.226 kb on - strand at 2712.489 kb on - strand at 2712.491 kb on - strand at 2712.491 kb on - strand at 2712.491 kb on - strand at 2712.533 kb on - strand at 2712.558 kb on - strand, within yfiE at 2712.620 kb on + strand, within yfiE at 2712.620 kb on + strand, within yfiE at 2712.653 kb on - strand, within yfiE at 2712.653 kb on - strand, within yfiE at 2712.745 kb on - strand, within yfiE at 2712.948 kb on - strand, within yfiE at 2713.040 kb on - strand, within yfiE at 2713.074 kb on - strand, within yfiE
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 2,709,919 + +0.2 2,709,942 - -0.1 2,709,942 - +1.7 2,709,949 + +0.4 2,709,954 + +0.8 2,709,957 - +0.2 2,709,997 + +0.4 2,710,005 - +0.4 2,710,005 - -0.7 2,710,005 - +0.2 2,710,189 - yfiC b2575 0.19 +0.3 2,710,236 + yfiC b2575 0.25 +0.1 2,710,329 + yfiC b2575 0.38 +0.0 2,710,375 - yfiC b2575 0.44 +0.2 2,710,398 + yfiC b2575 0.47 -0.6 2,710,399 - yfiC b2575 0.47 +0.9 2,710,399 - yfiC b2575 0.47 +1.2 2,710,402 - yfiC b2575 0.48 +0.3 2,710,409 + yfiC b2575 0.49 +0.1 2,710,410 + yfiC b2575 0.49 -1.2 2,710,413 - yfiC b2575 0.49 +0.1 2,710,413 - yfiC b2575 0.49 +0.2 2,710,418 - yfiC b2575 0.50 -0.4 2,710,418 - yfiC b2575 0.50 -0.0 2,710,439 - yfiC b2575 0.53 +0.5 2,710,439 - yfiC b2575 0.53 -0.1 2,710,591 - yfiC b2575 0.73 +1.2 2,710,770 + -0.3 2,710,770 + +0.1 2,710,770 + +0.2 2,710,770 + +0.1 2,710,778 - +0.6 2,710,778 - -0.3 2,710,833 - -0.7 2,710,833 - +0.0 2,710,838 + -0.7 2,711,086 + srmB b2576 0.13 -1.1 2,711,159 + srmB b2576 0.18 +1.7 2,711,181 + srmB b2576 0.20 -0.8 2,711,268 - srmB b2576 0.26 +1.7 2,711,305 + srmB b2576 0.29 +1.7 2,711,376 - srmB b2576 0.34 -0.6 2,711,425 - srmB b2576 0.38 -1.3 2,711,557 - srmB b2576 0.48 +0.7 2,711,666 + srmB b2576 0.56 -1.1 2,711,698 + srmB b2576 0.58 +0.2 2,711,747 + +0.2 2,711,849 + srmB b2576 0.70 +0.7 2,711,857 - srmB b2576 0.70 -0.3 2,711,857 - srmB b2576 0.70 -1.3 2,711,927 + srmB b2576 0.76 +0.7 2,712,112 - srmB b2576 0.89 +1.3 2,712,203 + +0.3 2,712,218 + -0.4 2,712,226 - +0.5 2,712,489 - +2.7 2,712,491 - +0.7 2,712,491 - -1.2 2,712,491 - +0.3 2,712,533 - +0.2 2,712,558 - yfiE b2577 0.11 -0.2 2,712,620 + yfiE b2577 0.18 -1.1 2,712,620 + yfiE b2577 0.18 -0.4 2,712,653 - yfiE b2577 0.22 -0.2 2,712,653 - yfiE b2577 0.22 -0.4 2,712,745 - yfiE b2577 0.32 -1.0 2,712,948 - yfiE b2577 0.55 +0.2 2,713,040 - yfiE b2577 0.66 +0.5 2,713,074 - yfiE b2577 0.70 -0.4
Or see this region's nucleotide sequence