Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rseC and rseB overlap by 4 nucleotides rseB and rseA overlap by 1 nucleotides rseA and rpoE are separated by 32 nucleotides
b2570: rseC - RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI), at 2,705,344 to 2,705,823
rseC
b2571: rseB - periplasmic negative regulator of sigmaE (NCBI), at 2,705,820 to 2,706,776
rseB
b2572: rseA - anti-sigma factor (NCBI), at 2,706,776 to 2,707,426
rseA
b2573: rpoE - RNA polymerase, sigma 24 (sigma E) factor (NCBI), at 2,707,459 to 2,708,034
rpoE
Position (kb)
2706
2707
2708 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2705.783 kb on - strand at 2705.783 kb on - strand at 2705.814 kb on - strand at 2705.915 kb on + strand at 2705.915 kb on + strand at 2705.923 kb on - strand, within rseB at 2705.987 kb on - strand, within rseB at 2706.013 kb on + strand, within rseB at 2706.190 kb on - strand, within rseB at 2706.190 kb on - strand, within rseB at 2706.211 kb on - strand, within rseB at 2706.307 kb on + strand, within rseB at 2706.315 kb on - strand, within rseB at 2706.315 kb on - strand, within rseB at 2706.322 kb on + strand, within rseB at 2706.397 kb on - strand, within rseB at 2706.399 kb on + strand, within rseB at 2706.524 kb on + strand, within rseB at 2706.524 kb on + strand, within rseB at 2706.576 kb on + strand, within rseB at 2706.601 kb on + strand, within rseB at 2706.601 kb on + strand, within rseB at 2706.654 kb on + strand, within rseB at 2706.696 kb on + strand at 2706.711 kb on - strand at 2706.711 kb on - strand at 2706.724 kb on - strand at 2706.738 kb on + strand at 2706.754 kb on - strand at 2706.754 kb on - strand at 2706.822 kb on - strand at 2706.822 kb on - strand at 2706.857 kb on - strand, within rseA at 2706.857 kb on - strand, within rseA at 2706.857 kb on - strand, within rseA at 2706.867 kb on - strand, within rseA at 2706.867 kb on - strand, within rseA at 2706.909 kb on - strand, within rseA at 2706.909 kb on - strand, within rseA at 2706.945 kb on - strand, within rseA at 2707.085 kb on - strand, within rseA at 2707.102 kb on - strand, within rseA at 2707.183 kb on + strand, within rseA at 2707.254 kb on + strand, within rseA at 2707.254 kb on + strand, within rseA at 2707.426 kb on - strand at 2707.436 kb on - strand at 2707.436 kb on - strand at 2707.437 kb on - strand at 2707.440 kb on + strand at 2708.179 kb on - strand at 2708.179 kb on - strand at 2708.218 kb on - strand at 2708.218 kb on - strand at 2708.221 kb on + strand at 2708.239 kb on - strand at 2708.289 kb on + strand at 2708.289 kb on + strand at 2708.309 kb on - strand at 2708.401 kb on - strand at 2708.406 kb on - strand at 2708.406 kb on - strand at 2708.411 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 2,705,783 - +3.7 2,705,783 - +1.4 2,705,814 - +0.2 2,705,915 + -3.5 2,705,915 + -0.3 2,705,923 - rseB b2571 0.11 -2.0 2,705,987 - rseB b2571 0.17 -0.8 2,706,013 + rseB b2571 0.20 -2.5 2,706,190 - rseB b2571 0.39 -0.7 2,706,190 - rseB b2571 0.39 -0.3 2,706,211 - rseB b2571 0.41 -1.6 2,706,307 + rseB b2571 0.51 -0.8 2,706,315 - rseB b2571 0.52 -0.1 2,706,315 - rseB b2571 0.52 -0.6 2,706,322 + rseB b2571 0.52 -0.9 2,706,397 - rseB b2571 0.60 -1.1 2,706,399 + rseB b2571 0.61 -1.7 2,706,524 + rseB b2571 0.74 +0.0 2,706,524 + rseB b2571 0.74 -1.8 2,706,576 + rseB b2571 0.79 -2.0 2,706,601 + rseB b2571 0.82 -2.2 2,706,601 + rseB b2571 0.82 -2.0 2,706,654 + rseB b2571 0.87 -1.4 2,706,696 + -1.7 2,706,711 - -0.5 2,706,711 - -1.3 2,706,724 - -1.1 2,706,738 + -2.0 2,706,754 - -0.9 2,706,754 - +1.0 2,706,822 - +0.0 2,706,822 - -2.0 2,706,857 - rseA b2572 0.12 -1.0 2,706,857 - rseA b2572 0.12 -0.1 2,706,857 - rseA b2572 0.12 -2.3 2,706,867 - rseA b2572 0.14 -0.9 2,706,867 - rseA b2572 0.14 -2.9 2,706,909 - rseA b2572 0.20 -2.5 2,706,909 - rseA b2572 0.20 -3.5 2,706,945 - rseA b2572 0.26 -0.8 2,707,085 - rseA b2572 0.47 -3.7 2,707,102 - rseA b2572 0.50 -3.2 2,707,183 + rseA b2572 0.63 +0.2 2,707,254 + rseA b2572 0.73 +0.2 2,707,254 + rseA b2572 0.73 -0.3 2,707,426 - +0.6 2,707,436 - +0.1 2,707,436 - -0.3 2,707,437 - +0.7 2,707,440 + +0.3 2,708,179 - -1.2 2,708,179 - -0.3 2,708,218 - -1.3 2,708,218 - -3.1 2,708,221 + +0.4 2,708,239 - -0.7 2,708,289 + +0.1 2,708,289 + -2.1 2,708,309 - -2.1 2,708,401 - -2.1 2,708,406 - -1.0 2,708,406 - -0.9 2,708,411 - -1.7
Or see this region's nucleotide sequence