Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b2511 and yfgL are separated by 117 nucleotides yfgL and yfgM are separated by 10 nucleotides yfgM and hisS are separated by 17 nucleotides
b2511: b2511 - putative GTP-binding factor (VIMSS), at 2,633,906 to 2,635,378
b2511
b2512: yfgL - protein assembly complex, lipoprotein component (NCBI), at 2,635,496 to 2,636,674
yfgL
b2513: yfgM - hypothetical protein (NCBI), at 2,636,685 to 2,637,305
yfgM
b2514: hisS - histidyl-tRNA synthetase (NCBI), at 2,637,323 to 2,638,597
hisS
Position (kb)
2635
2636
2637 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2635.526 kb on - strand at 2635.608 kb on - strand at 2635.787 kb on - strand, within yfgL at 2635.787 kb on - strand, within yfgL at 2635.892 kb on - strand, within yfgL at 2635.892 kb on - strand, within yfgL at 2636.030 kb on - strand, within yfgL at 2636.078 kb on - strand, within yfgL at 2636.167 kb on - strand, within yfgL at 2636.230 kb on - strand, within yfgL at 2636.230 kb on - strand, within yfgL at 2636.264 kb on - strand, within yfgL at 2636.264 kb on - strand, within yfgL at 2636.268 kb on - strand, within yfgL at 2636.268 kb on - strand, within yfgL at 2636.312 kb on - strand, within yfgL at 2636.355 kb on - strand, within yfgL at 2636.428 kb on - strand, within yfgL at 2636.428 kb on - strand, within yfgL at 2636.433 kb on + strand, within yfgL at 2636.440 kb on - strand, within yfgL at 2636.822 kb on - strand, within yfgM at 2636.857 kb on + strand, within yfgM at 2636.878 kb on + strand, within yfgM at 2636.878 kb on + strand, within yfgM at 2636.897 kb on - strand, within yfgM at 2636.899 kb on + strand, within yfgM at 2636.905 kb on - strand, within yfgM at 2636.905 kb on - strand, within yfgM at 2636.907 kb on - strand, within yfgM at 2636.965 kb on - strand, within yfgM at 2637.054 kb on - strand, within yfgM at 2637.269 kb on + strand at 2637.269 kb on + strand at 2637.312 kb on - strand at 2637.328 kb on - strand at 2637.334 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 2,635,526 - -3.5 2,635,608 - -1.4 2,635,787 - yfgL b2512 0.25 -2.1 2,635,787 - yfgL b2512 0.25 -2.4 2,635,892 - yfgL b2512 0.34 -2.0 2,635,892 - yfgL b2512 0.34 -1.3 2,636,030 - yfgL b2512 0.45 -1.7 2,636,078 - yfgL b2512 0.49 -3.2 2,636,167 - yfgL b2512 0.57 -1.4 2,636,230 - yfgL b2512 0.62 -2.0 2,636,230 - yfgL b2512 0.62 -2.5 2,636,264 - yfgL b2512 0.65 -0.9 2,636,264 - yfgL b2512 0.65 -1.1 2,636,268 - yfgL b2512 0.65 -2.2 2,636,268 - yfgL b2512 0.65 -2.2 2,636,312 - yfgL b2512 0.69 -2.4 2,636,355 - yfgL b2512 0.73 -2.9 2,636,428 - yfgL b2512 0.79 -2.3 2,636,428 - yfgL b2512 0.79 -2.4 2,636,433 + yfgL b2512 0.79 +1.7 2,636,440 - yfgL b2512 0.80 -1.1 2,636,822 - yfgM b2513 0.22 +0.4 2,636,857 + yfgM b2513 0.28 +0.1 2,636,878 + yfgM b2513 0.31 +0.1 2,636,878 + yfgM b2513 0.31 +0.7 2,636,897 - yfgM b2513 0.34 -1.1 2,636,899 + yfgM b2513 0.34 +0.7 2,636,905 - yfgM b2513 0.35 -0.3 2,636,905 - yfgM b2513 0.35 -0.3 2,636,907 - yfgM b2513 0.36 -0.4 2,636,965 - yfgM b2513 0.45 -1.0 2,637,054 - yfgM b2513 0.59 -0.9 2,637,269 + +0.1 2,637,269 + -1.4 2,637,312 - -0.6 2,637,328 - -1.0 2,637,334 - +0.3
Or see this region's nucleotide sequence