Experiment: LB Anaerobic with Spiramycin 0.000125 mM 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt eutI and eutT overlap by 4 nucleotides eutT and eutQ overlap by 4 nucleotides eutQ and eutP overlap by 26 nucleotides eutP and b2462 are separated by 12 nucleotides  
        
        b2458: eutI - predicted phosphotransacetylase subunit (NCBI), at 2,570,511 to 2,571,527 
         
        eutI 
         
        
        b2459: eutT - predicted cobalamin adenosyltransferase in ethanolamine utilization (NCBI), at 2,571,524 to 2,572,327 
         
        eutT 
         
        
        b2460: eutQ - hypothetical protein (NCBI), at 2,572,324 to 2,573,025 
         
        eutQ 
         
        
        b2461: eutP - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 2,573,000 to 2,573,479 
         
        eutP 
         
        
        b2462: b2462 - orf, hypothetical protein (VIMSS), at 2,573,492 to 2,573,827 
         
        b2462 
         Position (kb)  
2572 
 
2573 
 
2574 Strain fitness (log2 ratio)  
-2 
 
-1 
 
0 
 
1 
 
2 at 2571.330 kb on - strand, within eutI at 2571.337 kb on - strand, within eutI at 2571.337 kb on - strand, within eutI at 2571.441 kb on + strand at 2571.561 kb on + strand at 2571.561 kb on + strand at 2571.564 kb on - strand at 2571.599 kb on + strand at 2571.603 kb on - strand at 2571.603 kb on - strand at 2571.655 kb on + strand, within eutT at 2571.812 kb on + strand, within eutT at 2571.924 kb on + strand, within eutT at 2571.924 kb on + strand, within eutT at 2571.985 kb on - strand, within eutT at 2571.985 kb on - strand, within eutT at 2572.113 kb on + strand, within eutT at 2572.134 kb on + strand, within eutT at 2572.134 kb on + strand, within eutT at 2572.153 kb on - strand, within eutT at 2572.405 kb on + strand, within eutQ at 2572.471 kb on - strand, within eutQ at 2572.471 kb on - strand, within eutQ at 2572.477 kb on + strand, within eutQ at 2572.539 kb on + strand, within eutQ at 2572.539 kb on + strand, within eutQ at 2572.539 kb on + strand, within eutQ at 2572.539 kb on + strand, within eutQ at 2572.584 kb on - strand, within eutQ at 2573.126 kb on + strand, within eutP at 2573.186 kb on - strand, within eutP at 2573.186 kb on - strand, within eutP at 2573.222 kb on - strand, within eutP at 2573.225 kb on + strand, within eutP at 2573.225 kb on + strand, within eutP at 2573.237 kb on - strand, within eutP at 2573.260 kb on + strand, within eutP at 2573.260 kb on + strand, within eutP at 2573.278 kb on + strand, within eutP at 2573.278 kb on + strand, within eutP at 2573.279 kb on - strand, within eutP at 2573.281 kb on + strand, within eutP at 2573.281 kb on + strand, within eutP at 2573.281 kb on + strand, within eutP at 2573.281 kb on + strand, within eutP at 2573.281 kb on + strand, within eutP at 2573.281 kb on - strand, within eutP at 2573.281 kb on - strand, within eutP at 2573.282 kb on + strand, within eutP at 2573.282 kb on + strand, within eutP at 2573.283 kb on + strand, within eutP at 2573.283 kb on + strand, within eutP at 2573.283 kb on + strand, within eutP at 2573.284 kb on - strand, within eutP at 2573.286 kb on - strand, within eutP at 2573.291 kb on - strand, within eutP at 2573.291 kb on - strand, within eutP at 2573.304 kb on + strand, within eutP at 2573.304 kb on + strand, within eutP at 2573.304 kb on + strand, within eutP at 2573.312 kb on - strand, within eutP at 2573.315 kb on + strand, within eutP at 2573.355 kb on - strand, within eutP at 2573.356 kb on - strand, within eutP at 2573.399 kb on - strand, within eutP at 2573.399 kb on - strand, within eutP at 2573.459 kb on + strand at 2573.459 kb on - strand at 2573.459 kb on - strand at 2573.459 kb on - strand at 2573.496 kb on - strand at 2573.496 kb on - strand at 2573.496 kb on - strand at 2573.497 kb on - strand at 2573.550 kb on + strand, within b2462 at 2573.560 kb on - strand, within b2462 at 2573.562 kb on - strand, within b2462 at 2573.562 kb on - strand, within b2462 at 2573.562 kb on - strand, within b2462 at 2573.572 kb on - strand, within b2462 at 2573.572 kb on - strand, within b2462 at 2573.581 kb on - strand, within b2462 at 2573.581 kb on - strand, within b2462 at 2573.604 kb on - strand, within b2462 at 2573.606 kb on + strand, within b2462 at 2573.751 kb on - strand, within b2462 at 2573.774 kb on - strand, within b2462 at 2573.774 kb on - strand, within b2462 at 2573.775 kb on + strand, within b2462 at 2573.783 kb on - strand, within b2462 at 2573.784 kb on - strand, within b2462 at 2573.792 kb on - strand, within b2462 at 2573.818 kb on + strand at 2573.830 kb on + strand at 2573.830 kb on + strand at 2573.838 kb on - strand at 2573.838 kb on - strand at 2573.886 kb on + strand at 2573.928 kb on - strand at 2573.933 kb on - strand at 2573.963 kb on + strand at 2573.963 kb on + strand  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   LB Anaerobic with Spiramycin 0.000125 mM             remove   2,571,330   -  eutI  b2458   0.81  +0.4   2,571,337   -  eutI  b2458   0.81  -0.8   2,571,337   -  eutI  b2458   0.81  -1.0   2,571,441   +        +0.5   2,571,561   +        -1.5   2,571,561   +        +1.0   2,571,564   -        -0.1   2,571,599   +        +0.3   2,571,603   -        -1.6   2,571,603   -        -0.3   2,571,655   +  eutT  b2459   0.16  +0.7   2,571,812   +  eutT  b2459   0.36  -0.4   2,571,924   +  eutT  b2459   0.50  +0.5   2,571,924   +  eutT  b2459   0.50  +0.1   2,571,985   -  eutT  b2459   0.57  -0.2   2,571,985   -  eutT  b2459   0.57  -0.3   2,572,113   +  eutT  b2459   0.73  -0.6   2,572,134   +  eutT  b2459   0.76  -0.0   2,572,134   +  eutT  b2459   0.76  -0.8   2,572,153   -  eutT  b2459   0.78  -0.4   2,572,405   +  eutQ  b2460   0.12  -0.4   2,572,471   -  eutQ  b2460   0.21  -0.5   2,572,471   -  eutQ  b2460   0.21  +0.4   2,572,477   +  eutQ  b2460   0.22  +0.6   2,572,539   +  eutQ  b2460   0.31  -0.1   2,572,539   +  eutQ  b2460   0.31  +1.6   2,572,539   +  eutQ  b2460   0.31  -0.6   2,572,539   +  eutQ  b2460   0.31  -0.5   2,572,584   -  eutQ  b2460   0.37  +0.7   2,573,126   +  eutP  b2461   0.26  +0.6   2,573,186   -  eutP  b2461   0.39  -0.0   2,573,186   -  eutP  b2461   0.39  +0.7   2,573,222   -  eutP  b2461   0.46  +0.4   2,573,225   +  eutP  b2461   0.47  +1.3   2,573,225   +  eutP  b2461   0.47  -2.1   2,573,237   -  eutP  b2461   0.49  -0.2   2,573,260   +  eutP  b2461   0.54  +0.7   2,573,260   +  eutP  b2461   0.54  +0.1   2,573,278   +  eutP  b2461   0.58  -0.5   2,573,278   +  eutP  b2461   0.58  +0.0   2,573,279   -  eutP  b2461   0.58  +0.1   2,573,281   +  eutP  b2461   0.59  +0.5   2,573,281   +  eutP  b2461   0.59  -0.7   2,573,281   +  eutP  b2461   0.59  -0.3   2,573,281   +  eutP  b2461   0.59  -0.3   2,573,281   +  eutP  b2461   0.59  -0.2   2,573,281   -  eutP  b2461   0.59  +1.0   2,573,281   -  eutP  b2461   0.59  +1.7   2,573,282   +  eutP  b2461   0.59  -1.2   2,573,282   +  eutP  b2461   0.59  -0.1   2,573,283   +  eutP  b2461   0.59  -0.9   2,573,283   +  eutP  b2461   0.59  +0.2   2,573,283   +  eutP  b2461   0.59  -0.1   2,573,284   -  eutP  b2461   0.59  -0.2   2,573,286   -  eutP  b2461   0.60  -0.4   2,573,291   -  eutP  b2461   0.61  +0.5   2,573,291   -  eutP  b2461   0.61  -0.5   2,573,304   +  eutP  b2461   0.63  +0.2   2,573,304   +  eutP  b2461   0.63  -0.3   2,573,304   +  eutP  b2461   0.63  +0.1   2,573,312   -  eutP  b2461   0.65  +1.1   2,573,315   +  eutP  b2461   0.66  +0.0   2,573,355   -  eutP  b2461   0.74  +1.0   2,573,356   -  eutP  b2461   0.74  -2.7   2,573,399   -  eutP  b2461   0.83  -0.9   2,573,399   -  eutP  b2461   0.83  +0.6   2,573,459   +        -1.4   2,573,459   -        -0.4   2,573,459   -        -0.3   2,573,459   -        -0.6   2,573,496   -        +1.0   2,573,496   -        -1.6   2,573,496   -        +0.4   2,573,497   -        -0.5   2,573,550   +  b2462  b2462   0.17  +0.7   2,573,560   -  b2462  b2462   0.20  -0.7   2,573,562   -  b2462  b2462   0.21  -0.3   2,573,562   -  b2462  b2462   0.21  +0.9   2,573,562   -  b2462  b2462   0.21  -0.5   2,573,572   -  b2462  b2462   0.24  -0.1   2,573,572   -  b2462  b2462   0.24  -0.3   2,573,581   -  b2462  b2462   0.26  -0.2   2,573,581   -  b2462  b2462   0.26  +0.2   2,573,604   -  b2462  b2462   0.33  -0.5   2,573,606   +  b2462  b2462   0.34  +1.5   2,573,751   -  b2462  b2462   0.77  +0.5   2,573,774   -  b2462  b2462   0.84  -0.1   2,573,774   -  b2462  b2462   0.84  +2.0   2,573,775   +  b2462  b2462   0.84  +1.3   2,573,783   -  b2462  b2462   0.87  -0.2   2,573,784   -  b2462  b2462   0.87  +0.3   2,573,792   -  b2462  b2462   0.89  -0.5   2,573,818   +        +0.1   2,573,830   +        -0.2   2,573,830   +        +1.5   2,573,838   -        +0.7   2,573,838   -        -1.3   2,573,886   +        +0.0   2,573,928   -        +0.7   2,573,933   -        +1.7   2,573,963   +        +1.9   2,573,963   +        +1.7 
 
Or see this region's nucleotide sequence