Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b2294 and yfbV are separated by 82 nucleotides yfbV and ackA are separated by 337 nucleotides ackA and pta are separated by 74 nucleotides
b2294: b2294 - orf, hypothetical protein (VIMSS), at 2,410,122 to 2,410,616
b2294
b2295: yfbV - hypothetical protein (NCBI), at 2,410,699 to 2,411,154
yfbV
b2296: ackA - acetate kinase (NCBI), at 2,411,492 to 2,412,694
ackA
b2297: pta - phosphate acetyltransferase (NCBI), at 2,412,769 to 2,414,913
pta
Position (kb)
2411
2412
2413 Strain fitness (log2 ratio)
-2
-1
0
1 at 2410.508 kb on + strand, within b2294 at 2410.538 kb on - strand, within b2294 at 2410.538 kb on - strand, within b2294 at 2410.550 kb on + strand, within b2294 at 2410.561 kb on + strand, within b2294 at 2410.567 kb on + strand at 2410.567 kb on + strand at 2410.618 kb on + strand at 2410.623 kb on + strand at 2410.636 kb on - strand at 2410.636 kb on - strand at 2410.673 kb on + strand at 2410.673 kb on + strand at 2410.726 kb on - strand at 2410.765 kb on - strand, within yfbV at 2410.808 kb on + strand, within yfbV at 2410.808 kb on + strand, within yfbV at 2410.811 kb on - strand, within yfbV at 2410.858 kb on + strand, within yfbV at 2410.864 kb on + strand, within yfbV at 2410.866 kb on - strand, within yfbV at 2410.886 kb on + strand, within yfbV at 2410.934 kb on - strand, within yfbV at 2410.946 kb on - strand, within yfbV at 2410.948 kb on + strand, within yfbV at 2411.001 kb on + strand, within yfbV at 2411.113 kb on - strand at 2411.138 kb on - strand at 2411.174 kb on - strand at 2411.285 kb on - strand at 2411.290 kb on + strand at 2411.317 kb on + strand at 2411.398 kb on + strand at 2411.398 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 2,410,508 + b2294 b2294 0.78 -0.4 2,410,538 - b2294 b2294 0.84 +0.7 2,410,538 - b2294 b2294 0.84 +1.0 2,410,550 + b2294 b2294 0.86 +1.1 2,410,561 + b2294 b2294 0.89 +0.2 2,410,567 + -0.1 2,410,567 + +0.3 2,410,618 + +0.1 2,410,623 + -0.5 2,410,636 - +0.7 2,410,636 - +0.7 2,410,673 + -0.1 2,410,673 + -2.3 2,410,726 - -0.6 2,410,765 - yfbV b2295 0.14 -1.6 2,410,808 + yfbV b2295 0.24 +0.1 2,410,808 + yfbV b2295 0.24 +0.0 2,410,811 - yfbV b2295 0.25 +0.2 2,410,858 + yfbV b2295 0.35 -2.1 2,410,864 + yfbV b2295 0.36 -0.3 2,410,866 - yfbV b2295 0.37 -0.6 2,410,886 + yfbV b2295 0.41 +1.0 2,410,934 - yfbV b2295 0.52 -0.8 2,410,946 - yfbV b2295 0.54 -0.9 2,410,948 + yfbV b2295 0.55 +0.2 2,411,001 + yfbV b2295 0.66 -1.6 2,411,113 - +0.2 2,411,138 - -1.1 2,411,174 - -0.6 2,411,285 - -1.0 2,411,290 + -0.1 2,411,317 + -0.5 2,411,398 + -1.0 2,411,398 + -0.9
Or see this region's nucleotide sequence