Strain Fitness in Escherichia coli BW25113 around b2263

Experiment: LB Anaerobic with Spiramycin 0.000125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmenC and menB overlap by 1 nucleotidesmenB and yfbB are separated by 14 nucleotidesyfbB and menD overlap by 4 nucleotides b2261: menC - O-succinylbenzoate synthase (NCBI), at 2,373,022 to 2,373,984 menC b2262: menB - naphthoate synthase (NCBI), at 2,373,984 to 2,374,841 menB b2263: yfbB - predicted peptidase (NCBI), at 2,374,856 to 2,375,614 yfbB b2264: menD - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI), at 2,375,611 to 2,377,281 menD Position (kb) 2374 2375 2376Strain fitness (log2 ratio) -1 0 1at 2373.925 kb on + strandat 2373.930 kb on + strandat 2373.930 kb on + strandat 2374.136 kb on + strand, within menBat 2374.254 kb on - strand, within menBat 2374.373 kb on + strand, within menBat 2374.491 kb on + strand, within menBat 2374.673 kb on - strand, within menBat 2374.831 kb on + strandat 2374.831 kb on + strandat 2374.885 kb on + strandat 2374.893 kb on - strandat 2375.223 kb on - strand, within yfbBat 2375.307 kb on - strand, within yfbBat 2375.363 kb on - strand, within yfbBat 2375.570 kb on + strandat 2375.570 kb on + strandat 2375.616 kb on - strandat 2375.616 kb on - strandat 2375.795 kb on - strand, within menDat 2376.112 kb on - strand, within menDat 2376.143 kb on - strand, within menDat 2376.143 kb on - strand, within menDat 2376.359 kb on + strand, within menDat 2376.359 kb on + strand, within menDat 2376.515 kb on + strand, within menDat 2376.554 kb on - strand, within menDat 2376.585 kb on + strand, within menDat 2376.585 kb on + strand, within menDat 2376.605 kb on + strand, within menD

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM
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2,373,925 + +0.1
2,373,930 + +1.2
2,373,930 + +0.2
2,374,136 + menB b2262 0.18 -0.4
2,374,254 - menB b2262 0.31 +1.1
2,374,373 + menB b2262 0.45 +0.0
2,374,491 + menB b2262 0.59 -1.1
2,374,673 - menB b2262 0.80 +0.7
2,374,831 + +0.0
2,374,831 + -1.5
2,374,885 + +0.4
2,374,893 - -0.6
2,375,223 - yfbB b2263 0.48 +0.5
2,375,307 - yfbB b2263 0.59 +1.1
2,375,363 - yfbB b2263 0.67 +0.5
2,375,570 + +0.2
2,375,570 + +1.0
2,375,616 - -1.7
2,375,616 - -0.3
2,375,795 - menD b2264 0.11 +0.1
2,376,112 - menD b2264 0.30 -1.0
2,376,143 - menD b2264 0.32 +0.1
2,376,143 - menD b2264 0.32 +0.6
2,376,359 + menD b2264 0.45 -0.4
2,376,359 + menD b2264 0.45 +0.7
2,376,515 + menD b2264 0.54 -0.2
2,376,554 - menD b2264 0.56 -0.3
2,376,585 + menD b2264 0.58 +0.7
2,376,585 + menD b2264 0.58 +0.4
2,376,605 + menD b2264 0.59 +0.1

Or see this region's nucleotide sequence