Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bglX and dld are separated by 195 nucleotides dld and pbpG are separated by 37 nucleotides
b2132: bglX - beta-D-glucoside glucohydrolase, periplasmic (NCBI), at 2,217,714 to 2,220,011
bglX
b2133: dld - D-lactate dehydrogenase, FAD-binding, NADH independent (NCBI), at 2,220,207 to 2,221,922
dld
b2134: pbpG - penicillin-binding protein 7 (VIMSS), at 2,221,960 to 2,222,892
pbpG
Position (kb)
2220
2221
2222 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2219.307 kb on - strand, within bglX at 2219.343 kb on + strand, within bglX at 2219.398 kb on + strand, within bglX at 2219.398 kb on + strand, within bglX at 2219.407 kb on + strand, within bglX at 2219.415 kb on - strand, within bglX at 2219.539 kb on - strand, within bglX at 2219.559 kb on - strand, within bglX at 2219.659 kb on - strand, within bglX at 2219.664 kb on - strand, within bglX at 2219.891 kb on + strand at 2219.891 kb on + strand at 2219.969 kb on + strand at 2219.969 kb on + strand at 2219.977 kb on - strand at 2219.978 kb on - strand at 2219.998 kb on - strand at 2220.036 kb on - strand at 2220.147 kb on + strand at 2220.147 kb on + strand at 2220.167 kb on - strand at 2220.167 kb on - strand at 2220.206 kb on - strand at 2220.206 kb on - strand at 2220.220 kb on - strand at 2220.276 kb on - strand at 2220.276 kb on - strand at 2220.279 kb on - strand at 2220.330 kb on + strand at 2220.560 kb on - strand, within dld at 2220.623 kb on + strand, within dld at 2220.635 kb on + strand, within dld at 2220.651 kb on - strand, within dld at 2220.781 kb on + strand, within dld at 2220.781 kb on + strand, within dld at 2220.784 kb on - strand, within dld at 2220.784 kb on - strand, within dld at 2220.811 kb on + strand, within dld at 2221.087 kb on + strand, within dld at 2221.199 kb on + strand, within dld at 2221.199 kb on + strand, within dld at 2221.333 kb on + strand, within dld at 2221.333 kb on + strand, within dld at 2221.373 kb on + strand, within dld at 2221.373 kb on + strand, within dld at 2221.482 kb on - strand, within dld at 2221.505 kb on - strand, within dld at 2221.577 kb on + strand, within dld at 2221.658 kb on - strand, within dld at 2221.687 kb on + strand, within dld at 2221.697 kb on + strand, within dld at 2221.697 kb on + strand, within dld at 2221.730 kb on - strand, within dld at 2221.780 kb on + strand at 2221.781 kb on - strand at 2221.938 kb on - strand at 2222.020 kb on + strand at 2222.020 kb on + strand at 2222.154 kb on + strand, within pbpG at 2222.163 kb on + strand, within pbpG at 2222.171 kb on - strand, within pbpG at 2222.183 kb on + strand, within pbpG at 2222.254 kb on - strand, within pbpG at 2222.306 kb on + strand, within pbpG at 2222.314 kb on - strand, within pbpG at 2222.314 kb on - strand, within pbpG at 2222.314 kb on - strand, within pbpG at 2222.314 kb on - strand, within pbpG at 2222.552 kb on - strand, within pbpG at 2222.552 kb on - strand, within pbpG at 2222.602 kb on - strand, within pbpG at 2222.804 kb on - strand at 2222.806 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 2,219,307 - bglX b2132 0.69 -1.1 2,219,343 + bglX b2132 0.71 -0.6 2,219,398 + bglX b2132 0.73 -0.3 2,219,398 + bglX b2132 0.73 +1.1 2,219,407 + bglX b2132 0.74 +0.1 2,219,415 - bglX b2132 0.74 -1.7 2,219,539 - bglX b2132 0.79 -0.2 2,219,559 - bglX b2132 0.80 -0.3 2,219,659 - bglX b2132 0.85 -1.1 2,219,664 - bglX b2132 0.85 +0.4 2,219,891 + +1.1 2,219,891 + +0.1 2,219,969 + -1.6 2,219,969 + +1.1 2,219,977 - -0.6 2,219,978 - -0.2 2,219,998 - -0.4 2,220,036 - -0.2 2,220,147 + +2.2 2,220,147 + +1.0 2,220,167 - +0.0 2,220,167 - +0.8 2,220,206 - -1.0 2,220,206 - -0.6 2,220,220 - -0.2 2,220,276 - -0.2 2,220,276 - +0.5 2,220,279 - -1.1 2,220,330 + +0.4 2,220,560 - dld b2133 0.21 +0.3 2,220,623 + dld b2133 0.24 -0.4 2,220,635 + dld b2133 0.25 -0.9 2,220,651 - dld b2133 0.26 -0.2 2,220,781 + dld b2133 0.33 +0.0 2,220,781 + dld b2133 0.33 +0.0 2,220,784 - dld b2133 0.34 +1.0 2,220,784 - dld b2133 0.34 +0.9 2,220,811 + dld b2133 0.35 +0.6 2,221,087 + dld b2133 0.51 -0.7 2,221,199 + dld b2133 0.58 +0.3 2,221,199 + dld b2133 0.58 +0.1 2,221,333 + dld b2133 0.66 +0.0 2,221,333 + dld b2133 0.66 +1.2 2,221,373 + dld b2133 0.68 -1.8 2,221,373 + dld b2133 0.68 +0.5 2,221,482 - dld b2133 0.74 -0.4 2,221,505 - dld b2133 0.76 +1.9 2,221,577 + dld b2133 0.80 +0.2 2,221,658 - dld b2133 0.85 -0.2 2,221,687 + dld b2133 0.86 +0.3 2,221,697 + dld b2133 0.87 -0.1 2,221,697 + dld b2133 0.87 -0.1 2,221,730 - dld b2133 0.89 -0.1 2,221,780 + -0.3 2,221,781 - +0.2 2,221,938 - +0.8 2,222,020 + +0.5 2,222,020 + +0.8 2,222,154 + pbpG b2134 0.21 +0.6 2,222,163 + pbpG b2134 0.22 +1.2 2,222,171 - pbpG b2134 0.23 -0.2 2,222,183 + pbpG b2134 0.24 +0.9 2,222,254 - pbpG b2134 0.32 +0.9 2,222,306 + pbpG b2134 0.37 -0.4 2,222,314 - pbpG b2134 0.38 +0.7 2,222,314 - pbpG b2134 0.38 +0.1 2,222,314 - pbpG b2134 0.38 +0.8 2,222,314 - pbpG b2134 0.38 +0.6 2,222,552 - pbpG b2134 0.63 +0.7 2,222,552 - pbpG b2134 0.63 +0.5 2,222,602 - pbpG b2134 0.69 +0.9 2,222,804 - +1.1 2,222,806 - -0.5
Or see this region's nucleotide sequence