Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hdhA and malI are separated by 111 nucleotides malI and malX are separated by 174 nucleotides
b1619: hdhA - 7-alpha-hydroxysteroid dehydrogenase (NCBI), at 1,695,297 to 1,696,064
hdhA
b1620: malI - DNA-binding transcriptional repressor (NCBI), at 1,696,176 to 1,697,204
malI
b1621: malX - fused maltose and glucose-specific PTS enzymes: IIB component -! IIC component (NCBI), at 1,697,379 to 1,698,971
malX
Position (kb)
1696
1697
1698 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1695.234 kb on - strand at 1695.316 kb on - strand at 1695.368 kb on + strand at 1695.368 kb on + strand at 1695.393 kb on - strand, within hdhA at 1695.425 kb on + strand, within hdhA at 1695.432 kb on - strand, within hdhA at 1695.432 kb on - strand, within hdhA at 1695.558 kb on - strand, within hdhA at 1695.574 kb on + strand, within hdhA at 1695.574 kb on + strand, within hdhA at 1695.574 kb on + strand, within hdhA at 1695.582 kb on - strand, within hdhA at 1695.582 kb on - strand, within hdhA at 1695.592 kb on - strand, within hdhA at 1695.592 kb on - strand, within hdhA at 1695.690 kb on - strand, within hdhA at 1695.690 kb on - strand, within hdhA at 1695.724 kb on - strand, within hdhA at 1695.758 kb on - strand, within hdhA at 1695.771 kb on - strand, within hdhA at 1695.771 kb on - strand, within hdhA at 1695.779 kb on - strand, within hdhA at 1695.779 kb on - strand, within hdhA at 1695.841 kb on - strand, within hdhA at 1695.841 kb on - strand, within hdhA at 1695.901 kb on - strand, within hdhA at 1695.914 kb on - strand, within hdhA at 1695.925 kb on - strand, within hdhA at 1695.959 kb on + strand, within hdhA at 1695.959 kb on + strand, within hdhA at 1696.001 kb on - strand at 1696.125 kb on - strand at 1696.125 kb on - strand at 1696.151 kb on - strand at 1696.154 kb on + strand at 1696.154 kb on + strand at 1696.154 kb on + strand at 1696.161 kb on - strand at 1696.161 kb on - strand at 1696.163 kb on - strand at 1696.163 kb on - strand at 1696.176 kb on + strand at 1696.176 kb on + strand at 1696.181 kb on - strand at 1696.181 kb on - strand at 1696.314 kb on + strand, within malI at 1696.330 kb on - strand, within malI at 1696.599 kb on - strand, within malI at 1696.617 kb on + strand, within malI at 1696.643 kb on + strand, within malI at 1697.394 kb on + strand at 1697.437 kb on - strand at 1697.538 kb on - strand at 1697.538 kb on - strand at 1697.580 kb on - strand, within malX at 1697.580 kb on - strand, within malX at 1697.869 kb on - strand, within malX
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 1,695,234 - +0.3 1,695,316 - -2.3 1,695,368 + -0.3 1,695,368 + +0.8 1,695,393 - hdhA b1619 0.12 +0.1 1,695,425 + hdhA b1619 0.17 +1.8 1,695,432 - hdhA b1619 0.18 -2.2 1,695,432 - hdhA b1619 0.18 -0.6 1,695,558 - hdhA b1619 0.34 +0.6 1,695,574 + hdhA b1619 0.36 +1.5 1,695,574 + hdhA b1619 0.36 -2.1 1,695,574 + hdhA b1619 0.36 +0.5 1,695,582 - hdhA b1619 0.37 -1.3 1,695,582 - hdhA b1619 0.37 +1.5 1,695,592 - hdhA b1619 0.38 +0.7 1,695,592 - hdhA b1619 0.38 +1.8 1,695,690 - hdhA b1619 0.51 +0.4 1,695,690 - hdhA b1619 0.51 +0.6 1,695,724 - hdhA b1619 0.56 +0.5 1,695,758 - hdhA b1619 0.60 -0.1 1,695,771 - hdhA b1619 0.62 +1.5 1,695,771 - hdhA b1619 0.62 -0.4 1,695,779 - hdhA b1619 0.63 +1.0 1,695,779 - hdhA b1619 0.63 -0.3 1,695,841 - hdhA b1619 0.71 -1.1 1,695,841 - hdhA b1619 0.71 +0.2 1,695,901 - hdhA b1619 0.79 -0.2 1,695,914 - hdhA b1619 0.80 -1.1 1,695,925 - hdhA b1619 0.82 -0.5 1,695,959 + hdhA b1619 0.86 -0.5 1,695,959 + hdhA b1619 0.86 -1.1 1,696,001 - +0.9 1,696,125 - -1.7 1,696,125 - -0.5 1,696,151 - +0.6 1,696,154 + -0.8 1,696,154 + +0.5 1,696,154 + +0.5 1,696,161 - +0.6 1,696,161 - +0.7 1,696,163 - +0.4 1,696,163 - -0.3 1,696,176 + +0.5 1,696,176 + -0.5 1,696,181 - -0.5 1,696,181 - +0.6 1,696,314 + malI b1620 0.13 -1.6 1,696,330 - malI b1620 0.15 -0.5 1,696,599 - malI b1620 0.41 -1.1 1,696,617 + malI b1620 0.43 -1.1 1,696,643 + malI b1620 0.45 +0.4 1,697,394 + -0.2 1,697,437 - +0.6 1,697,538 - -1.3 1,697,538 - -0.6 1,697,580 - malX b1621 0.13 -0.1 1,697,580 - malX b1621 0.13 +0.4 1,697,869 - malX b1621 0.31 +0.7
Or see this region's nucleotide sequence