Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt intQ and rspB are separated by 187 nucleotides rspB and rspA are separated by 11 nucleotides
b1579: intQ - Qin prophage; predicted defective integrase (RefSeq), at 1,649,575 to 1,650,732
intQ
b1580: rspB - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI), at 1,650,920 to 1,651,939
rspB
b1581: rspA - predicted dehydratase (NCBI), at 1,651,951 to 1,653,165
rspA
Position (kb)
1650
1651
1652 Strain fitness (log2 ratio)
-1
0
1 at 1649.958 kb on + strand, within intQ at 1649.963 kb on + strand, within intQ at 1649.966 kb on - strand, within intQ at 1649.966 kb on - strand, within intQ at 1649.972 kb on - strand, within intQ at 1649.993 kb on - strand, within intQ at 1650.079 kb on - strand, within intQ at 1650.126 kb on - strand, within intQ at 1650.151 kb on - strand, within intQ at 1650.153 kb on + strand, within intQ at 1650.153 kb on + strand, within intQ at 1650.153 kb on + strand, within intQ at 1650.161 kb on - strand, within intQ at 1650.161 kb on - strand, within intQ at 1650.164 kb on + strand, within intQ at 1650.165 kb on - strand, within intQ at 1650.178 kb on + strand, within intQ at 1650.236 kb on - strand, within intQ at 1650.274 kb on + strand, within intQ at 1650.298 kb on + strand, within intQ at 1650.313 kb on + strand, within intQ at 1650.313 kb on + strand, within intQ at 1650.363 kb on + strand, within intQ at 1650.477 kb on - strand, within intQ at 1650.533 kb on + strand, within intQ at 1650.536 kb on + strand, within intQ at 1650.546 kb on + strand, within intQ at 1650.546 kb on + strand, within intQ at 1650.619 kb on + strand at 1650.690 kb on - strand at 1650.792 kb on + strand at 1650.984 kb on - strand at 1651.150 kb on + strand, within rspB at 1651.158 kb on - strand, within rspB at 1651.163 kb on - strand, within rspB at 1651.193 kb on + strand, within rspB at 1651.211 kb on + strand, within rspB at 1651.211 kb on + strand, within rspB at 1651.219 kb on - strand, within rspB at 1651.609 kb on + strand, within rspB at 1651.618 kb on - strand, within rspB at 1651.667 kb on - strand, within rspB at 1651.677 kb on - strand, within rspB at 1651.728 kb on + strand, within rspB at 1651.728 kb on + strand, within rspB at 1651.728 kb on + strand, within rspB at 1651.728 kb on - strand, within rspB at 1651.735 kb on - strand, within rspB at 1651.740 kb on + strand, within rspB at 1651.802 kb on - strand, within rspB at 1651.859 kb on + strand at 1651.974 kb on + strand at 1652.048 kb on - strand at 1652.060 kb on - strand at 1652.070 kb on + strand at 1652.070 kb on + strand at 1652.237 kb on - strand, within rspA at 1652.628 kb on - strand, within rspA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 1,649,958 + intQ b1579 0.33 -0.5 1,649,963 + intQ b1579 0.34 +1.5 1,649,966 - intQ b1579 0.34 -1.5 1,649,966 - intQ b1579 0.34 +0.9 1,649,972 - intQ b1579 0.34 -0.5 1,649,993 - intQ b1579 0.36 +1.5 1,650,079 - intQ b1579 0.44 +1.5 1,650,126 - intQ b1579 0.48 +0.5 1,650,151 - intQ b1579 0.50 +1.5 1,650,153 + intQ b1579 0.50 +1.5 1,650,153 + intQ b1579 0.50 +1.5 1,650,153 + intQ b1579 0.50 +0.5 1,650,161 - intQ b1579 0.51 +1.5 1,650,161 - intQ b1579 0.51 +1.5 1,650,164 + intQ b1579 0.51 +1.5 1,650,165 - intQ b1579 0.51 -0.5 1,650,178 + intQ b1579 0.52 -0.1 1,650,236 - intQ b1579 0.57 +1.5 1,650,274 + intQ b1579 0.60 +1.5 1,650,298 + intQ b1579 0.62 +0.5 1,650,313 + intQ b1579 0.64 +0.5 1,650,313 + intQ b1579 0.64 +1.5 1,650,363 + intQ b1579 0.68 -0.6 1,650,477 - intQ b1579 0.78 +1.5 1,650,533 + intQ b1579 0.83 -1.1 1,650,536 + intQ b1579 0.83 +0.5 1,650,546 + intQ b1579 0.84 +1.5 1,650,546 + intQ b1579 0.84 +1.5 1,650,619 + +1.5 1,650,690 - +1.5 1,650,792 + +1.5 1,650,984 - +1.5 1,651,150 + rspB b1580 0.23 -1.1 1,651,158 - rspB b1580 0.23 -0.5 1,651,163 - rspB b1580 0.24 +0.5 1,651,193 + rspB b1580 0.27 -0.1 1,651,211 + rspB b1580 0.29 -0.9 1,651,211 + rspB b1580 0.29 -1.1 1,651,219 - rspB b1580 0.29 +0.0 1,651,609 + rspB b1580 0.68 -0.8 1,651,618 - rspB b1580 0.68 +0.4 1,651,667 - rspB b1580 0.73 -0.8 1,651,677 - rspB b1580 0.74 -0.9 1,651,728 + rspB b1580 0.79 -0.5 1,651,728 + rspB b1580 0.79 -0.2 1,651,728 + rspB b1580 0.79 +0.4 1,651,728 - rspB b1580 0.79 +0.5 1,651,735 - rspB b1580 0.80 +0.4 1,651,740 + rspB b1580 0.80 -0.5 1,651,802 - rspB b1580 0.86 -1.1 1,651,859 + -1.5 1,651,974 + +0.9 1,652,048 - -0.0 1,652,060 - +0.8 1,652,070 + -0.2 1,652,070 + -0.6 1,652,237 - rspA b1581 0.24 +0.5 1,652,628 - rspA b1581 0.56 +0.5
Or see this region's nucleotide sequence