Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydcP and yncJ are separated by 91 nucleotides yncJ and yncN are separated by 221 nucleotides yncN and ydcQ are separated by 45 nucleotides ydcQ and ydcR are separated by 78 nucleotides
b1435: ydcP - putative collagenase (VIMSS), at 1,504,805 to 1,506,766
ydcP
b1436: yncJ - hypothetical protein (NCBI), at 1,506,858 to 1,507,088
yncJ
b4532: yncN - orf, hypothetical protein (VIMSS), at 1,507,310 to 1,507,486
yncN
b1438: ydcQ - predicted DNA-binding transcriptional regulator (RefSeq), at 1,507,532 to 1,507,948
ydcQ
b1439: ydcR - fused predicted DNA-binding transcriptional regulator/predicted amino transferase (NCBI), at 1,508,027 to 1,509,433
ydcR
Position (kb)
1507
1508 Strain fitness (log2 ratio)
-2
-1
0
1 at 1506.768 kb on - strand at 1506.768 kb on - strand at 1507.385 kb on - strand, within yncN at 1507.407 kb on + strand, within yncN at 1507.450 kb on + strand, within yncN at 1507.631 kb on + strand, within ydcQ at 1507.675 kb on + strand, within ydcQ at 1507.702 kb on + strand, within ydcQ at 1507.710 kb on - strand, within ydcQ at 1507.761 kb on + strand, within ydcQ at 1507.819 kb on + strand, within ydcQ at 1508.082 kb on + strand at 1508.082 kb on + strand at 1508.230 kb on - strand, within ydcR at 1508.233 kb on - strand, within ydcR at 1508.314 kb on + strand, within ydcR at 1508.314 kb on + strand, within ydcR at 1508.403 kb on + strand, within ydcR at 1508.479 kb on - strand, within ydcR at 1508.509 kb on - strand, within ydcR at 1508.632 kb on + strand, within ydcR at 1508.820 kb on + strand, within ydcR at 1508.820 kb on + strand, within ydcR
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 1,506,768 - -0.1 1,506,768 - -0.5 1,507,385 - yncN b4532 0.42 -0.5 1,507,407 + yncN b4532 0.55 -0.2 1,507,450 + yncN b4532 0.79 +0.1 1,507,631 + ydcQ b1438 0.24 -2.1 1,507,675 + ydcQ b1438 0.34 -2.1 1,507,702 + ydcQ b1438 0.41 -1.4 1,507,710 - ydcQ b1438 0.43 +0.7 1,507,761 + ydcQ b1438 0.55 +0.5 1,507,819 + ydcQ b1438 0.69 -1.1 1,508,082 + -0.0 1,508,082 + -1.3 1,508,230 - ydcR b1439 0.14 -0.5 1,508,233 - ydcR b1439 0.15 -0.9 1,508,314 + ydcR b1439 0.20 +0.7 1,508,314 + ydcR b1439 0.20 +0.2 1,508,403 + ydcR b1439 0.27 +0.6 1,508,479 - ydcR b1439 0.32 +0.7 1,508,509 - ydcR b1439 0.34 -0.0 1,508,632 + ydcR b1439 0.43 +0.2 1,508,820 + ydcR b1439 0.56 -0.6 1,508,820 + ydcR b1439 0.56 -0.1
Or see this region's nucleotide sequence