Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ndh and ycfJ are separated by 209 nucleotides ycfJ and ycfQ are separated by 61 nucleotides ycfQ and ycfR are separated by 240 nucleotides ycfR and ycfS are separated by 81 nucleotides
b1109: ndh - respiratory NADH dehydrogenase 2/cupric reductase (NCBI), at 1,165,308 to 1,166,612
ndh
b1110: ycfJ - hypothetical protein (NCBI), at 1,166,822 to 1,167,361
ycfJ
b1111: ycfQ - orf, hypothetical protein (VIMSS), at 1,167,423 to 1,168,055
ycfQ
b1112: ycfR - hypothetical protein (NCBI), at 1,168,296 to 1,168,553
ycfR
b1113: ycfS - hypothetical protein (NCBI), at 1,168,635 to 1,169,597
ycfS
Position (kb)
1167
1168
1169 Strain fitness (log2 ratio)
-2
-1
0
1 at 1166.478 kb on - strand, within ndh at 1166.501 kb on - strand at 1166.526 kb on + strand at 1166.573 kb on - strand at 1166.623 kb on - strand at 1166.655 kb on + strand at 1166.661 kb on - strand at 1166.824 kb on + strand at 1166.972 kb on + strand, within ycfJ at 1166.972 kb on + strand, within ycfJ at 1166.972 kb on + strand, within ycfJ at 1166.972 kb on + strand, within ycfJ at 1166.980 kb on + strand, within ycfJ at 1167.051 kb on + strand, within ycfJ at 1167.051 kb on + strand, within ycfJ at 1167.125 kb on + strand, within ycfJ at 1167.132 kb on + strand, within ycfJ at 1167.132 kb on + strand, within ycfJ at 1167.243 kb on + strand, within ycfJ at 1167.243 kb on + strand, within ycfJ at 1167.243 kb on + strand, within ycfJ at 1167.251 kb on - strand, within ycfJ at 1167.263 kb on - strand, within ycfJ at 1167.263 kb on - strand, within ycfJ at 1167.362 kb on - strand at 1167.364 kb on + strand at 1167.364 kb on + strand at 1167.640 kb on + strand, within ycfQ at 1167.840 kb on + strand, within ycfQ at 1167.840 kb on - strand, within ycfQ at 1167.949 kb on - strand, within ycfQ at 1168.045 kb on - strand at 1168.045 kb on - strand at 1168.075 kb on - strand at 1168.075 kb on - strand at 1168.746 kb on + strand, within ycfS at 1168.766 kb on + strand, within ycfS at 1168.802 kb on + strand, within ycfS at 1168.805 kb on + strand, within ycfS at 1168.870 kb on + strand, within ycfS at 1168.925 kb on - strand, within ycfS at 1169.013 kb on + strand, within ycfS
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 1,166,478 - ndh b1109 0.90 +0.0 1,166,501 - +0.9 1,166,526 + +0.2 1,166,573 - -0.5 1,166,623 - -0.3 1,166,655 + +0.7 1,166,661 - -0.3 1,166,824 + +1.0 1,166,972 + ycfJ b1110 0.28 +1.6 1,166,972 + ycfJ b1110 0.28 +0.4 1,166,972 + ycfJ b1110 0.28 -1.5 1,166,972 + ycfJ b1110 0.28 +0.9 1,166,980 + ycfJ b1110 0.29 +0.5 1,167,051 + ycfJ b1110 0.42 -0.7 1,167,051 + ycfJ b1110 0.42 -0.7 1,167,125 + ycfJ b1110 0.56 +0.3 1,167,132 + ycfJ b1110 0.57 -0.5 1,167,132 + ycfJ b1110 0.57 +0.3 1,167,243 + ycfJ b1110 0.78 +0.2 1,167,243 + ycfJ b1110 0.78 +1.6 1,167,243 + ycfJ b1110 0.78 -1.4 1,167,251 - ycfJ b1110 0.79 -0.1 1,167,263 - ycfJ b1110 0.82 +0.4 1,167,263 - ycfJ b1110 0.82 +0.4 1,167,362 - -1.5 1,167,364 + -0.4 1,167,364 + +1.0 1,167,640 + ycfQ b1111 0.34 +0.0 1,167,840 + ycfQ b1111 0.66 -0.9 1,167,840 - ycfQ b1111 0.66 +0.6 1,167,949 - ycfQ b1111 0.83 -2.6 1,168,045 - +0.5 1,168,045 - +0.5 1,168,075 - -0.3 1,168,075 - -0.2 1,168,746 + ycfS b1113 0.12 +0.3 1,168,766 + ycfS b1113 0.14 -1.2 1,168,802 + ycfS b1113 0.17 +0.7 1,168,805 + ycfS b1113 0.18 +1.2 1,168,870 + ycfS b1113 0.24 -0.4 1,168,925 - ycfS b1113 0.30 -0.9 1,169,013 + ycfS b1113 0.39 +1.0
Or see this region's nucleotide sequence