Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pflB and focA are separated by 54 nucleotides focA and ycaO are separated by 405 nucleotides
b0903: pflB - pyruvate formate lyase I (NCBI), at 950,495 to 952,777
pflB
b0904: focA - formate transporter (NCBI), at 952,832 to 953,689
focA
b0905: ycaO - orf, hypothetical protein (VIMSS), at 954,095 to 955,855
ycaO
Position (kb)
952
953
954 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 951.905 kb on - strand, within pflB at 951.961 kb on - strand, within pflB at 951.963 kb on - strand, within pflB at 951.963 kb on - strand, within pflB at 952.102 kb on - strand, within pflB at 952.107 kb on - strand, within pflB at 952.107 kb on - strand, within pflB at 952.132 kb on + strand, within pflB at 952.170 kb on + strand, within pflB at 952.170 kb on + strand, within pflB at 952.178 kb on - strand, within pflB at 952.178 kb on - strand, within pflB at 952.183 kb on - strand, within pflB at 952.256 kb on + strand, within pflB at 952.256 kb on + strand, within pflB at 952.352 kb on + strand, within pflB at 952.405 kb on - strand, within pflB at 952.444 kb on + strand, within pflB at 952.508 kb on + strand, within pflB at 952.521 kb on - strand, within pflB at 952.527 kb on - strand, within pflB at 952.776 kb on + strand at 952.983 kb on - strand, within focA at 952.983 kb on - strand, within focA at 953.066 kb on - strand, within focA at 953.096 kb on + strand, within focA at 953.155 kb on - strand, within focA at 953.163 kb on - strand, within focA at 953.175 kb on + strand, within focA at 953.175 kb on + strand, within focA at 953.193 kb on + strand, within focA at 953.246 kb on + strand, within focA at 953.423 kb on - strand, within focA at 953.423 kb on - strand, within focA at 953.514 kb on + strand, within focA at 953.516 kb on - strand, within focA at 953.516 kb on - strand, within focA at 953.557 kb on + strand, within focA at 953.566 kb on - strand, within focA at 953.581 kb on + strand, within focA at 953.610 kb on + strand at 953.611 kb on + strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.659 kb on + strand at 953.682 kb on + strand at 953.682 kb on + strand at 953.691 kb on - strand at 953.697 kb on - strand at 953.774 kb on + strand at 953.774 kb on + strand at 953.774 kb on + strand at 953.892 kb on + strand at 953.916 kb on + strand at 953.972 kb on + strand at 954.045 kb on + strand at 954.064 kb on - strand at 954.068 kb on + strand at 954.072 kb on + strand at 954.073 kb on + strand at 954.073 kb on + strand at 954.076 kb on - strand at 954.076 kb on - strand at 954.076 kb on - strand at 954.076 kb on - strand at 954.076 kb on - strand at 954.081 kb on - strand at 954.081 kb on - strand at 954.085 kb on - strand at 954.124 kb on + strand at 954.138 kb on - strand at 954.147 kb on + strand at 954.147 kb on + strand at 954.158 kb on + strand at 954.172 kb on + strand at 954.172 kb on + strand at 954.188 kb on + strand at 954.188 kb on + strand at 954.240 kb on + strand at 954.240 kb on + strand at 954.258 kb on + strand at 954.260 kb on + strand at 954.260 kb on + strand at 954.263 kb on - strand at 954.318 kb on + strand, within ycaO at 954.394 kb on - strand, within ycaO at 954.394 kb on - strand, within ycaO at 954.402 kb on + strand, within ycaO at 954.402 kb on + strand, within ycaO at 954.402 kb on + strand, within ycaO at 954.410 kb on - strand, within ycaO at 954.418 kb on + strand, within ycaO at 954.426 kb on - strand, within ycaO at 954.426 kb on - strand, within ycaO at 954.458 kb on - strand, within ycaO at 954.483 kb on + strand, within ycaO at 954.491 kb on - strand, within ycaO at 954.637 kb on + strand, within ycaO
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 951,905 - pflB b0903 0.62 -2.7 951,961 - pflB b0903 0.64 -2.1 951,963 - pflB b0903 0.64 -3.1 951,963 - pflB b0903 0.64 -2.1 952,102 - pflB b0903 0.70 -1.8 952,107 - pflB b0903 0.71 -2.7 952,107 - pflB b0903 0.71 -0.9 952,132 + pflB b0903 0.72 -2.4 952,170 + pflB b0903 0.73 -0.9 952,170 + pflB b0903 0.73 -0.7 952,178 - pflB b0903 0.74 -4.0 952,178 - pflB b0903 0.74 -2.8 952,183 - pflB b0903 0.74 -2.1 952,256 + pflB b0903 0.77 -1.8 952,256 + pflB b0903 0.77 -4.7 952,352 + pflB b0903 0.81 -3.5 952,405 - pflB b0903 0.84 -2.8 952,444 + pflB b0903 0.85 -1.4 952,508 + pflB b0903 0.88 -2.8 952,521 - pflB b0903 0.89 -2.2 952,527 - pflB b0903 0.89 -2.4 952,776 + -3.5 952,983 - focA b0904 0.18 -2.1 952,983 - focA b0904 0.18 -0.1 953,066 - focA b0904 0.27 -1.4 953,096 + focA b0904 0.31 -0.7 953,155 - focA b0904 0.38 -0.3 953,163 - focA b0904 0.39 -0.5 953,175 + focA b0904 0.40 -1.8 953,175 + focA b0904 0.40 -0.9 953,193 + focA b0904 0.42 -0.2 953,246 + focA b0904 0.48 -2.1 953,423 - focA b0904 0.69 +0.5 953,423 - focA b0904 0.69 +0.3 953,514 + focA b0904 0.79 -1.8 953,516 - focA b0904 0.80 -0.9 953,516 - focA b0904 0.80 -1.8 953,557 + focA b0904 0.84 -1.3 953,566 - focA b0904 0.86 +0.6 953,581 + focA b0904 0.87 -0.6 953,610 + -1.5 953,611 + -2.2 953,618 - -1.2 953,618 - -0.3 953,618 - -0.5 953,618 - -0.7 953,618 - +0.8 953,618 - +1.1 953,618 - -2.3 953,618 - -1.7 953,618 - +0.6 953,659 + -1.1 953,682 + +0.1 953,682 + -0.8 953,691 - +0.6 953,697 - +0.1 953,774 + +0.6 953,774 + +1.4 953,774 + +0.4 953,892 + -0.6 953,916 + +0.2 953,972 + -0.3 954,045 + -0.6 954,064 - -0.6 954,068 + +1.6 954,072 + +1.6 954,073 + +0.6 954,073 + +1.6 954,076 - +0.1 954,076 - +0.1 954,076 - +0.4 954,076 - +1.2 954,076 - -0.3 954,081 - +1.1 954,081 - +0.5 954,085 - +0.2 954,124 + +0.3 954,138 - +0.1 954,147 + +1.1 954,147 + -0.4 954,158 + +1.0 954,172 + +0.1 954,172 + -0.0 954,188 + -1.9 954,188 + +0.4 954,240 + -0.5 954,240 + -0.7 954,258 + -0.1 954,260 + -1.5 954,260 + -0.4 954,263 - -0.1 954,318 + ycaO b0905 0.13 +0.6 954,394 - ycaO b0905 0.17 -0.0 954,394 - ycaO b0905 0.17 -0.2 954,402 + ycaO b0905 0.17 -0.4 954,402 + ycaO b0905 0.17 -0.4 954,402 + ycaO b0905 0.17 +0.1 954,410 - ycaO b0905 0.18 +0.2 954,418 + ycaO b0905 0.18 +0.1 954,426 - ycaO b0905 0.19 +0.5 954,426 - ycaO b0905 0.19 +0.0 954,458 - ycaO b0905 0.21 -0.0 954,483 + ycaO b0905 0.22 +1.1 954,491 - ycaO b0905 0.22 +0.1 954,637 + ycaO b0905 0.31 -1.2
Or see this region's nucleotide sequence