Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rhtA and ompX are separated by 352 nucleotides ompX and ybiP are separated by 48 nucleotides
b0813: rhtA - threonine and homoserine efflux system (NCBI), at 848,433 to 849,320
rhtA
b0814: ompX - outer membrane protein X (NCBI), at 849,673 to 850,188
ompX
b0815: ybiP - predicted hydrolase, inner membrane (NCBI), at 850,237 to 851,820
ybiP
Position (kb) 849
850
851 Strain fitness (log2 ratio) -2
-1
0
1 at 848.695 kb on - strand, within rhtA at 848.695 kb on - strand, within rhtA at 848.695 kb on - strand, within rhtA at 848.717 kb on - strand, within rhtA at 848.717 kb on - strand, within rhtA at 848.717 kb on - strand, within rhtA at 848.718 kb on + strand, within rhtA at 848.718 kb on + strand, within rhtA at 848.772 kb on - strand, within rhtA at 848.772 kb on - strand, within rhtA at 848.796 kb on + strand, within rhtA at 848.809 kb on - strand, within rhtA at 848.809 kb on - strand, within rhtA at 848.905 kb on - strand, within rhtA at 848.991 kb on + strand, within rhtA at 848.991 kb on + strand, within rhtA at 849.017 kb on - strand, within rhtA at 849.017 kb on - strand, within rhtA at 849.028 kb on - strand, within rhtA at 849.108 kb on + strand, within rhtA at 849.202 kb on + strand, within rhtA at 849.202 kb on + strand, within rhtA at 849.227 kb on + strand, within rhtA at 849.248 kb on + strand at 849.272 kb on + strand at 849.284 kb on - strand at 849.319 kb on - strand at 849.324 kb on - strand at 849.371 kb on - strand at 849.371 kb on - strand at 849.374 kb on + strand at 849.698 kb on - strand at 849.739 kb on - strand, within ompX at 849.809 kb on + strand, within ompX at 849.850 kb on - strand, within ompX at 849.893 kb on + strand, within ompX at 849.902 kb on + strand, within ompX at 849.902 kb on + strand, within ompX at 849.965 kb on + strand, within ompX at 849.990 kb on - strand, within ompX at 850.018 kb on + strand, within ompX at 850.019 kb on + strand, within ompX at 850.027 kb on - strand, within ompX at 850.273 kb on + strand at 850.281 kb on - strand at 850.342 kb on - strand at 850.384 kb on - strand at 850.412 kb on + strand, within ybiP at 850.448 kb on - strand, within ybiP at 850.544 kb on - strand, within ybiP at 850.544 kb on - strand, within ybiP at 850.557 kb on - strand, within ybiP at 850.594 kb on + strand, within ybiP at 850.594 kb on + strand, within ybiP at 850.600 kb on + strand, within ybiP at 850.706 kb on + strand, within ybiP at 850.755 kb on + strand, within ybiP at 850.755 kb on + strand, within ybiP at 851.041 kb on - strand, within ybiP at 851.041 kb on - strand, within ybiP at 851.157 kb on + strand, within ybiP at 851.157 kb on + strand, within ybiP
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 848,695 - rhtA b0813 0.30 -0.6 848,695 - rhtA b0813 0.30 -0.6 848,695 - rhtA b0813 0.30 +1.0 848,717 - rhtA b0813 0.32 +1.6 848,717 - rhtA b0813 0.32 +0.5 848,717 - rhtA b0813 0.32 -0.0 848,718 + rhtA b0813 0.32 -0.1 848,718 + rhtA b0813 0.32 +0.8 848,772 - rhtA b0813 0.38 -0.4 848,772 - rhtA b0813 0.38 -0.2 848,796 + rhtA b0813 0.41 +0.3 848,809 - rhtA b0813 0.42 +0.8 848,809 - rhtA b0813 0.42 +0.2 848,905 - rhtA b0813 0.53 -0.3 848,991 + rhtA b0813 0.63 -1.0 848,991 + rhtA b0813 0.63 -0.6 849,017 - rhtA b0813 0.66 +1.1 849,017 - rhtA b0813 0.66 +0.1 849,028 - rhtA b0813 0.67 -1.2 849,108 + rhtA b0813 0.76 -0.2 849,202 + rhtA b0813 0.87 -0.2 849,202 + rhtA b0813 0.87 -0.6 849,227 + rhtA b0813 0.89 -0.3 849,248 + -0.2 849,272 + -0.7 849,284 - +0.0 849,319 - +0.5 849,324 - +0.2 849,371 - -0.2 849,371 - -0.8 849,374 + +0.8 849,698 - +1.7 849,739 - ompX b0814 0.13 +1.7 849,809 + ompX b0814 0.26 -1.0 849,850 - ompX b0814 0.34 -0.6 849,893 + ompX b0814 0.43 +1.7 849,902 + ompX b0814 0.44 -0.3 849,902 + ompX b0814 0.44 -0.6 849,965 + ompX b0814 0.57 -0.3 849,990 - ompX b0814 0.61 +0.1 850,018 + ompX b0814 0.67 -1.8 850,019 + ompX b0814 0.67 -1.0 850,027 - ompX b0814 0.69 -0.2 850,273 + -1.0 850,281 - +0.0 850,342 - +0.4 850,384 - -0.8 850,412 + ybiP b0815 0.11 +0.8 850,448 - ybiP b0815 0.13 -0.8 850,544 - ybiP b0815 0.19 -0.0 850,544 - ybiP b0815 0.19 +1.1 850,557 - ybiP b0815 0.20 -1.7 850,594 + ybiP b0815 0.23 -1.8 850,594 + ybiP b0815 0.23 +0.1 850,600 + ybiP b0815 0.23 +0.7 850,706 + ybiP b0815 0.30 -0.9 850,755 + ybiP b0815 0.33 -0.3 850,755 + ybiP b0815 0.33 -0.3 851,041 - ybiP b0815 0.51 -1.1 851,041 - ybiP b0815 0.51 +0.5 851,157 + ybiP b0815 0.58 +1.1 851,157 + ybiP b0815 0.58 +1.3
Or see this region's nucleotide sequence