Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolA and tolB are separated by 132 nucleotides tolB and pal are separated by 34 nucleotides pal and ybgF are separated by 9 nucleotides
b0739: tolA - cell envelope integrity inner membrane protein TolA (NCBI), at 775,565 to 776,830
tolA
b0740: tolB - translocation protein TolB precursor (NCBI), at 776,963 to 778,255
tolB
b0741: pal - peptidoglycan-associated outer membrane lipoprotein (NCBI), at 778,290 to 778,811
pal
b0742: ybgF - hypothetical protein (NCBI), at 778,821 to 779,612
ybgF
Position (kb)
776
777
778
779 Strain fitness (log2 ratio)
-2
-1
0
1 at 776.098 kb on - strand, within tolA at 776.124 kb on + strand, within tolA at 776.146 kb on + strand, within tolA at 776.265 kb on - strand, within tolA at 776.265 kb on - strand, within tolA at 776.464 kb on + strand, within tolA at 776.475 kb on - strand, within tolA at 776.601 kb on - strand, within tolA at 776.625 kb on - strand, within tolA at 776.640 kb on - strand, within tolA at 776.640 kb on - strand, within tolA at 776.657 kb on - strand, within tolA at 776.713 kb on - strand at 776.725 kb on + strand at 776.779 kb on - strand at 776.943 kb on + strand at 776.968 kb on - strand at 776.994 kb on + strand at 776.994 kb on + strand at 777.012 kb on + strand at 777.014 kb on + strand at 777.135 kb on + strand, within tolB at 777.146 kb on + strand, within tolB at 777.260 kb on + strand, within tolB at 777.278 kb on - strand, within tolB at 777.299 kb on - strand, within tolB at 777.303 kb on - strand, within tolB at 777.393 kb on - strand, within tolB at 777.398 kb on - strand, within tolB at 777.444 kb on - strand, within tolB at 777.513 kb on + strand, within tolB at 777.522 kb on - strand, within tolB at 777.602 kb on - strand, within tolB at 777.602 kb on - strand, within tolB at 777.692 kb on + strand, within tolB at 777.700 kb on - strand, within tolB at 777.720 kb on - strand, within tolB at 777.770 kb on - strand, within tolB at 777.792 kb on - strand, within tolB at 777.839 kb on - strand, within tolB at 777.952 kb on - strand, within tolB at 777.954 kb on - strand, within tolB at 777.987 kb on - strand, within tolB at 778.009 kb on - strand, within tolB at 778.014 kb on - strand, within tolB at 778.020 kb on - strand, within tolB at 778.070 kb on + strand, within tolB at 778.127 kb on - strand at 778.150 kb on - strand at 778.150 kb on - strand at 778.212 kb on + strand at 778.212 kb on + strand at 778.212 kb on + strand at 778.220 kb on - strand at 778.222 kb on + strand at 778.316 kb on + strand at 778.316 kb on + strand at 778.382 kb on + strand, within pal at 778.420 kb on - strand, within pal at 778.422 kb on - strand, within pal at 778.455 kb on + strand, within pal at 778.455 kb on + strand, within pal at 778.560 kb on + strand, within pal at 778.568 kb on - strand, within pal at 778.596 kb on - strand, within pal at 778.600 kb on + strand, within pal at 778.604 kb on - strand, within pal at 778.656 kb on - strand, within pal at 778.656 kb on - strand at 778.661 kb on - strand, within pal at 778.713 kb on - strand, within pal at 778.752 kb on + strand, within pal at 778.752 kb on + strand, within pal at 778.752 kb on + strand, within pal at 778.780 kb on + strand at 778.780 kb on + strand at 778.805 kb on - strand at 778.851 kb on + strand at 778.856 kb on + strand at 778.864 kb on - strand at 778.870 kb on + strand at 778.932 kb on + strand, within ybgF at 778.940 kb on - strand, within ybgF at 778.940 kb on - strand, within ybgF at 778.940 kb on - strand, within ybgF at 778.940 kb on - strand, within ybgF at 779.036 kb on + strand, within ybgF at 779.145 kb on + strand, within ybgF at 779.145 kb on + strand, within ybgF at 779.158 kb on - strand, within ybgF at 779.196 kb on + strand, within ybgF at 779.201 kb on + strand, within ybgF at 779.201 kb on + strand, within ybgF at 779.204 kb on - strand, within ybgF
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 776,098 - tolA b0739 0.42 -1.1 776,124 + tolA b0739 0.44 +0.7 776,146 + tolA b0739 0.46 +0.7 776,265 - tolA b0739 0.55 -0.8 776,265 - tolA b0739 0.55 +0.7 776,464 + tolA b0739 0.71 -0.8 776,475 - tolA b0739 0.72 -0.3 776,601 - tolA b0739 0.82 +0.7 776,625 - tolA b0739 0.84 -0.8 776,640 - tolA b0739 0.85 -1.3 776,640 - tolA b0739 0.85 -0.3 776,657 - tolA b0739 0.86 +0.7 776,713 - -0.6 776,725 + -0.3 776,779 - -0.3 776,943 + +0.1 776,968 - -0.3 776,994 + +1.7 776,994 + +0.7 777,012 + -0.6 777,014 + +0.7 777,135 + tolB b0740 0.13 +0.2 777,146 + tolB b0740 0.14 -2.4 777,260 + tolB b0740 0.23 +0.2 777,278 - tolB b0740 0.24 -0.8 777,299 - tolB b0740 0.26 -1.8 777,303 - tolB b0740 0.26 -0.3 777,393 - tolB b0740 0.33 -2.2 777,398 - tolB b0740 0.34 +0.7 777,444 - tolB b0740 0.37 +0.2 777,513 + tolB b0740 0.43 +0.7 777,522 - tolB b0740 0.43 +1.7 777,602 - tolB b0740 0.49 +0.7 777,602 - tolB b0740 0.49 -0.3 777,692 + tolB b0740 0.56 -1.3 777,700 - tolB b0740 0.57 -1.4 777,720 - tolB b0740 0.59 -1.3 777,770 - tolB b0740 0.62 -1.1 777,792 - tolB b0740 0.64 +1.7 777,839 - tolB b0740 0.68 -0.1 777,952 - tolB b0740 0.76 -0.8 777,954 - tolB b0740 0.77 +0.7 777,987 - tolB b0740 0.79 -1.4 778,009 - tolB b0740 0.81 -1.3 778,014 - tolB b0740 0.81 -0.8 778,020 - tolB b0740 0.82 +1.7 778,070 + tolB b0740 0.86 -1.4 778,127 - +0.7 778,150 - -1.4 778,150 - +0.2 778,212 + +1.7 778,212 + +0.2 778,212 + +0.7 778,220 - +0.2 778,222 + -1.8 778,316 + -0.3 778,316 + -1.1 778,382 + pal b0741 0.18 -0.6 778,420 - pal b0741 0.25 +0.2 778,422 - pal b0741 0.25 -0.3 778,455 + pal b0741 0.32 -0.6 778,455 + pal b0741 0.32 -0.6 778,560 + pal b0741 0.52 -0.4 778,568 - pal b0741 0.53 -0.4 778,596 - pal b0741 0.59 -0.3 778,600 + pal b0741 0.59 +0.7 778,604 - pal b0741 0.60 -0.6 778,656 - pal b0741 0.70 +1.7 778,656 - +0.7 778,661 - pal b0741 0.71 -2.3 778,713 - pal b0741 0.81 -1.4 778,752 + pal b0741 0.89 -1.3 778,752 + pal b0741 0.89 -0.8 778,752 + pal b0741 0.89 +1.1 778,780 + -1.6 778,780 + -1.1 778,805 - -2.6 778,851 + -0.1 778,856 + -1.1 778,864 - +1.7 778,870 + -0.5 778,932 + ybgF b0742 0.14 +0.6 778,940 - ybgF b0742 0.15 -0.7 778,940 - ybgF b0742 0.15 +0.3 778,940 - ybgF b0742 0.15 -0.8 778,940 - ybgF b0742 0.15 +0.2 779,036 + ybgF b0742 0.27 -1.0 779,145 + ybgF b0742 0.41 -0.9 779,145 + ybgF b0742 0.41 +0.1 779,158 - ybgF b0742 0.43 +0.5 779,196 + ybgF b0742 0.47 +0.2 779,201 + ybgF b0742 0.48 -0.1 779,201 + ybgF b0742 0.48 +1.7 779,204 - ybgF b0742 0.48 +0.5
Or see this region's nucleotide sequence