Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagC and nagA are separated by 8 nucleotides nagA and nagB are separated by 59 nucleotides
b0676: nagC - DNA-binding transcriptional dual regulator, repressor of N-acetylglucosamine (NCBI), at 699,597 to 700,817
nagC
b0677: nagA - N-acetylglucosamine-6-phosphate deacetylase (NCBI), at 700,826 to 701,974
nagA
b0678: nagB - glucosamine-6-phosphate deaminase (NCBI), at 702,034 to 702,834
nagB
Position (kb) 700
701
702 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 699.844 kb on + strand, within nagC at 699.854 kb on + strand, within nagC at 699.939 kb on + strand, within nagC at 699.966 kb on - strand, within nagC at 699.966 kb on - strand, within nagC at 699.968 kb on + strand, within nagC at 700.083 kb on + strand, within nagC at 700.083 kb on + strand, within nagC at 700.132 kb on - strand, within nagC at 700.132 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.206 kb on - strand, within nagC at 700.206 kb on - strand, within nagC at 700.269 kb on + strand, within nagC at 700.454 kb on + strand, within nagC at 700.486 kb on + strand, within nagC at 700.542 kb on - strand, within nagC at 700.726 kb on - strand at 700.726 kb on - strand at 700.733 kb on + strand at 700.759 kb on + strand at 700.759 kb on + strand at 700.761 kb on + strand at 700.813 kb on - strand at 700.813 kb on - strand at 700.967 kb on - strand, within nagA at 700.977 kb on - strand, within nagA at 701.025 kb on - strand, within nagA at 701.074 kb on - strand, within nagA at 701.191 kb on + strand, within nagA at 701.244 kb on - strand, within nagA at 701.309 kb on - strand, within nagA at 701.309 kb on - strand, within nagA at 701.321 kb on + strand, within nagA at 701.327 kb on - strand, within nagA at 701.347 kb on - strand, within nagA at 701.382 kb on + strand, within nagA at 701.417 kb on + strand, within nagA at 701.498 kb on - strand, within nagA at 701.531 kb on - strand, within nagA at 701.565 kb on + strand, within nagA at 701.565 kb on + strand, within nagA at 701.648 kb on + strand, within nagA at 701.692 kb on + strand, within nagA at 701.694 kb on - strand, within nagA at 701.755 kb on + strand, within nagA at 701.766 kb on + strand, within nagA at 701.805 kb on - strand, within nagA at 701.829 kb on + strand, within nagA at 701.904 kb on - strand at 701.904 kb on - strand at 701.959 kb on + strand at 701.959 kb on + strand at 701.959 kb on + strand at 701.968 kb on + strand at 701.980 kb on - strand at 702.001 kb on + strand at 702.001 kb on + strand at 702.011 kb on - strand at 702.020 kb on - strand at 702.104 kb on + strand at 702.160 kb on - strand, within nagB at 702.160 kb on - strand, within nagB at 702.166 kb on + strand, within nagB at 702.180 kb on - strand, within nagB at 702.217 kb on - strand, within nagB at 702.250 kb on + strand, within nagB at 702.346 kb on + strand, within nagB at 702.619 kb on - strand, within nagB at 702.624 kb on + strand, within nagB at 702.624 kb on + strand, within nagB at 702.769 kb on + strand at 702.794 kb on + strand at 702.804 kb on + strand at 702.823 kb on + strand at 702.823 kb on + strand at 702.881 kb on + strand at 702.966 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 699,844 + nagC b0676 0.20 +1.3 699,854 + nagC b0676 0.21 -1.6 699,939 + nagC b0676 0.28 -0.2 699,966 - nagC b0676 0.30 -1.5 699,966 - nagC b0676 0.30 +0.9 699,968 + nagC b0676 0.30 +0.6 700,083 + nagC b0676 0.40 -1.8 700,083 + nagC b0676 0.40 -1.2 700,132 - nagC b0676 0.44 -1.0 700,132 - nagC b0676 0.44 +0.1 700,193 - nagC b0676 0.49 -1.2 700,193 - nagC b0676 0.49 +0.1 700,193 - nagC b0676 0.49 +0.4 700,206 - nagC b0676 0.50 +2.8 700,206 - nagC b0676 0.50 -0.6 700,269 + nagC b0676 0.55 -0.6 700,454 + nagC b0676 0.70 +0.6 700,486 + nagC b0676 0.73 +0.6 700,542 - nagC b0676 0.77 +0.0 700,726 - +0.8 700,726 - +0.9 700,733 + -0.8 700,759 + -0.8 700,759 + -0.6 700,761 + -0.6 700,813 - -0.4 700,813 - -0.8 700,967 - nagA b0677 0.12 -1.6 700,977 - nagA b0677 0.13 -3.6 701,025 - nagA b0677 0.17 -1.8 701,074 - nagA b0677 0.22 +0.2 701,191 + nagA b0677 0.32 -1.2 701,244 - nagA b0677 0.36 -2.5 701,309 - nagA b0677 0.42 -1.2 701,309 - nagA b0677 0.42 -1.2 701,321 + nagA b0677 0.43 -0.6 701,327 - nagA b0677 0.44 -1.0 701,347 - nagA b0677 0.45 -0.4 701,382 + nagA b0677 0.48 -0.8 701,417 + nagA b0677 0.51 -0.6 701,498 - nagA b0677 0.58 -2.8 701,531 - nagA b0677 0.61 -1.9 701,565 + nagA b0677 0.64 -0.8 701,565 + nagA b0677 0.64 -3.1 701,648 + nagA b0677 0.72 -0.8 701,692 + nagA b0677 0.75 +0.2 701,694 - nagA b0677 0.76 -1.0 701,755 + nagA b0677 0.81 +0.8 701,766 + nagA b0677 0.82 +0.8 701,805 - nagA b0677 0.85 -4.1 701,829 + nagA b0677 0.87 -0.8 701,904 - -1.2 701,904 - -0.7 701,959 + -2.5 701,959 + -1.4 701,959 + -0.2 701,968 + -0.7 701,980 - -0.6 702,001 + +0.2 702,001 + -0.6 702,011 - -0.0 702,020 - +2.0 702,104 + +0.6 702,160 - nagB b0678 0.16 +1.3 702,160 - nagB b0678 0.16 +1.0 702,166 + nagB b0678 0.16 -0.1 702,180 - nagB b0678 0.18 -0.0 702,217 - nagB b0678 0.23 +0.8 702,250 + nagB b0678 0.27 +0.3 702,346 + nagB b0678 0.39 -0.1 702,619 - nagB b0678 0.73 +0.5 702,624 + nagB b0678 0.74 +0.3 702,624 + nagB b0678 0.74 +0.9 702,769 + +0.8 702,794 + -1.7 702,804 + -0.1 702,823 + +0.5 702,823 + -0.7 702,881 + +1.0 702,966 + +0.8
Or see this region's nucleotide sequence