Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybcY and tfaX are separated by 146 nucleotides tfaX and appY are separated by 545 nucleotides appY and ompT are separated by 249 nucleotides
b0562: ybcY - orf, hypothetical protein (VIMSS), at 581,375 to 582,029
ybcY
b0563: tfaX - DLP12 prophage; predicted protein (RefSeq), at 582,176 to 582,358
tfaX
b0564: appY - DLP12 prophage; DNA-binding transcriptional activator (NCBI), at 582,904 to 583,653
appY
b0565: ompT - DLP12 prophage; outer membrane protease VII (outer membrane protein 3b) (NCBI), at 583,903 to 584,856
ompT
Position (kb) 582
583
584 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 581.920 kb on + strand, within ybcY at 581.920 kb on + strand, within ybcY at 581.920 kb on - strand, within ybcY at 581.925 kb on - strand, within ybcY at 581.925 kb on - strand, within ybcY at 581.928 kb on - strand, within ybcY at 581.928 kb on - strand, within ybcY at 581.930 kb on + strand, within ybcY at 581.930 kb on + strand, within ybcY at 581.930 kb on + strand, within ybcY at 581.930 kb on + strand, within ybcY at 581.930 kb on + strand, within ybcY at 581.932 kb on + strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.940 kb on + strand, within ybcY at 581.969 kb on + strand at 581.970 kb on + strand at 581.970 kb on + strand at 581.970 kb on + strand at 581.977 kb on + strand at 581.977 kb on + strand at 581.992 kb on - strand at 582.013 kb on + strand at 582.104 kb on + strand at 582.104 kb on + strand at 582.138 kb on + strand at 582.140 kb on - strand at 582.147 kb on + strand at 582.147 kb on + strand at 582.151 kb on - strand at 582.155 kb on - strand at 582.163 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.176 kb on + strand at 582.177 kb on + strand at 582.182 kb on - strand at 582.182 kb on - strand at 582.182 kb on - strand at 582.182 kb on - strand at 582.182 kb on - strand at 582.184 kb on + strand at 582.184 kb on + strand at 582.185 kb on - strand at 582.187 kb on - strand at 582.190 kb on - strand at 582.190 kb on - strand at 582.208 kb on + strand, within tfaX at 582.208 kb on + strand, within tfaX at 582.210 kb on + strand, within tfaX at 582.210 kb on + strand, within tfaX at 582.220 kb on + strand, within tfaX at 582.220 kb on + strand, within tfaX at 582.221 kb on + strand, within tfaX at 582.221 kb on + strand, within tfaX at 582.226 kb on + strand, within tfaX at 582.236 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.301 kb on + strand, within tfaX at 582.306 kb on + strand, within tfaX at 582.306 kb on + strand, within tfaX at 582.307 kb on + strand, within tfaX at 582.318 kb on + strand, within tfaX at 582.318 kb on + strand, within tfaX at 582.326 kb on + strand, within tfaX at 583.029 kb on + strand, within appY at 583.247 kb on - strand, within appY at 583.916 kb on - strand at 583.922 kb on - strand at 583.922 kb on - strand at 583.923 kb on - strand at 583.923 kb on - strand at 584.038 kb on - strand, within ompT at 584.038 kb on - strand, within ompT at 584.038 kb on - strand, within ompT at 584.058 kb on - strand, within ompT at 584.113 kb on - strand, within ompT at 584.162 kb on - strand, within ompT at 584.162 kb on - strand, within ompT at 584.164 kb on - strand, within ompT at 584.169 kb on - strand, within ompT at 584.179 kb on - strand, within ompT at 584.179 kb on - strand, within ompT at 584.234 kb on - strand, within ompT at 584.248 kb on - strand, within ompT at 584.258 kb on - strand, within ompT at 584.258 kb on - strand, within ompT at 584.258 kb on - strand, within ompT at 584.258 kb on - strand, within ompT at 584.262 kb on - strand, within ompT at 584.262 kb on - strand, within ompT at 584.263 kb on - strand, within ompT at 584.263 kb on - strand, within ompT at 584.304 kb on - strand, within ompT at 584.304 kb on - strand, within ompT at 584.310 kb on - strand, within ompT at 584.327 kb on - strand, within ompT at 584.334 kb on - strand, within ompT at 584.337 kb on + strand, within ompT at 584.345 kb on - strand, within ompT at 584.345 kb on - strand, within ompT at 584.350 kb on - strand, within ompT at 584.352 kb on + strand, within ompT at 584.358 kb on - strand, within ompT at 584.358 kb on - strand, within ompT at 584.362 kb on + strand, within ompT at 584.370 kb on - strand, within ompT at 584.370 kb on - strand, within ompT at 584.371 kb on - strand, within ompT at 584.377 kb on + strand, within ompT at 584.391 kb on + strand, within ompT at 584.398 kb on - strand, within ompT at 584.399 kb on - strand, within ompT at 584.407 kb on + strand, within ompT at 584.415 kb on + strand, within ompT at 584.423 kb on - strand, within ompT at 584.423 kb on - strand, within ompT at 584.444 kb on - strand, within ompT at 584.492 kb on - strand, within ompT at 584.492 kb on - strand, within ompT at 584.494 kb on - strand, within ompT at 584.494 kb on - strand, within ompT at 584.500 kb on - strand, within ompT at 584.500 kb on - strand, within ompT at 584.516 kb on + strand, within ompT at 584.524 kb on - strand, within ompT at 584.524 kb on - strand, within ompT at 584.531 kb on + strand, within ompT at 584.537 kb on + strand, within ompT at 584.537 kb on + strand, within ompT at 584.537 kb on + strand, within ompT at 584.545 kb on - strand, within ompT at 584.564 kb on - strand, within ompT at 584.564 kb on - strand, within ompT at 584.565 kb on - strand, within ompT at 584.566 kb on - strand, within ompT at 584.566 kb on - strand, within ompT at 584.572 kb on + strand, within ompT at 584.572 kb on + strand, within ompT at 584.577 kb on - strand, within ompT at 584.580 kb on + strand, within ompT at 584.581 kb on + strand, within ompT at 584.582 kb on + strand, within ompT at 584.584 kb on - strand, within ompT at 584.587 kb on + strand, within ompT at 584.587 kb on + strand, within ompT at 584.589 kb on + strand, within ompT at 584.590 kb on - strand, within ompT at 584.608 kb on - strand, within ompT at 584.612 kb on - strand, within ompT at 584.612 kb on - strand, within ompT
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 581,920 + ybcY b0562 0.83 +1.2 581,920 + ybcY b0562 0.83 +2.0 581,920 - ybcY b0562 0.83 +0.2 581,925 - ybcY b0562 0.84 +2.5 581,925 - ybcY b0562 0.84 -1.4 581,928 - ybcY b0562 0.84 -0.2 581,928 - ybcY b0562 0.84 -0.9 581,930 + ybcY b0562 0.85 -0.2 581,930 + ybcY b0562 0.85 +0.6 581,930 + ybcY b0562 0.85 +0.5 581,930 + ybcY b0562 0.85 -0.2 581,930 + ybcY b0562 0.85 +0.7 581,932 + ybcY b0562 0.85 +0.3 581,938 - ybcY b0562 0.86 -1.0 581,938 - ybcY b0562 0.86 -0.5 581,938 - ybcY b0562 0.86 -0.0 581,938 - ybcY b0562 0.86 +1.2 581,938 - ybcY b0562 0.86 +0.9 581,938 - ybcY b0562 0.86 +0.8 581,938 - ybcY b0562 0.86 -0.2 581,940 + ybcY b0562 0.86 -0.2 581,969 + +0.8 581,970 + +1.8 581,970 + -2.0 581,970 + -0.2 581,977 + +0.4 581,977 + +0.5 581,992 - +1.8 582,013 + +0.1 582,104 + -0.5 582,104 + -1.5 582,138 + -0.7 582,140 - +1.8 582,147 + +0.2 582,147 + +0.2 582,151 - -1.2 582,155 - +0.8 582,163 + -0.7 582,174 + -0.8 582,174 + -1.4 582,174 + +0.5 582,174 + -1.0 582,174 + +0.1 582,174 + -0.5 582,174 + +1.2 582,176 + +0.6 582,177 + -0.5 582,182 - +0.8 582,182 - +1.8 582,182 - +1.8 582,182 - +1.0 582,182 - +1.8 582,184 + +0.6 582,184 + +0.4 582,185 - +1.8 582,187 - +0.8 582,190 - +1.8 582,190 - +1.8 582,208 + tfaX b0563 0.17 +0.3 582,208 + tfaX b0563 0.17 +1.0 582,210 + tfaX b0563 0.19 +0.8 582,210 + tfaX b0563 0.19 +0.5 582,220 + tfaX b0563 0.24 -0.5 582,220 + tfaX b0563 0.24 -0.5 582,221 + tfaX b0563 0.25 +0.6 582,221 + tfaX b0563 0.25 -1.0 582,226 + tfaX b0563 0.27 +0.1 582,236 + tfaX b0563 0.33 -0.1 582,299 + tfaX b0563 0.67 -0.0 582,299 + tfaX b0563 0.67 -0.8 582,299 + tfaX b0563 0.67 -2.4 582,299 + tfaX b0563 0.67 +1.5 582,299 + tfaX b0563 0.67 +1.8 582,299 + tfaX b0563 0.67 -0.8 582,299 + tfaX b0563 0.67 +0.6 582,299 + tfaX b0563 0.67 +0.8 582,299 + tfaX b0563 0.67 -0.0 582,301 + tfaX b0563 0.68 -1.4 582,306 + tfaX b0563 0.71 -0.5 582,306 + tfaX b0563 0.71 -1.1 582,307 + tfaX b0563 0.72 +0.0 582,318 + tfaX b0563 0.78 -0.6 582,318 + tfaX b0563 0.78 -0.5 582,326 + tfaX b0563 0.82 -1.0 583,029 + appY b0564 0.17 +0.8 583,247 - appY b0564 0.46 -1.7 583,916 - -0.5 583,922 - -0.7 583,922 - +1.2 583,923 - -0.9 583,923 - +0.3 584,038 - ompT b0565 0.14 -2.3 584,038 - ompT b0565 0.14 -2.3 584,038 - ompT b0565 0.14 -0.8 584,058 - ompT b0565 0.16 -2.4 584,113 - ompT b0565 0.22 -1.2 584,162 - ompT b0565 0.27 -0.7 584,162 - ompT b0565 0.27 -0.1 584,164 - ompT b0565 0.27 -0.4 584,169 - ompT b0565 0.28 -1.0 584,179 - ompT b0565 0.29 -0.3 584,179 - ompT b0565 0.29 +0.7 584,234 - ompT b0565 0.35 -3.1 584,248 - ompT b0565 0.36 -0.3 584,258 - ompT b0565 0.37 -0.6 584,258 - ompT b0565 0.37 -0.7 584,258 - ompT b0565 0.37 +0.1 584,258 - ompT b0565 0.37 -0.8 584,262 - ompT b0565 0.38 -1.0 584,262 - ompT b0565 0.38 +0.3 584,263 - ompT b0565 0.38 -2.1 584,263 - ompT b0565 0.38 -1.2 584,304 - ompT b0565 0.42 +1.0 584,304 - ompT b0565 0.42 -1.3 584,310 - ompT b0565 0.43 +0.3 584,327 - ompT b0565 0.44 -0.7 584,334 - ompT b0565 0.45 +0.2 584,337 + ompT b0565 0.45 +1.8 584,345 - ompT b0565 0.46 +1.1 584,345 - ompT b0565 0.46 -0.4 584,350 - ompT b0565 0.47 -0.0 584,352 + ompT b0565 0.47 +1.8 584,358 - ompT b0565 0.48 +0.6 584,358 - ompT b0565 0.48 -0.4 584,362 + ompT b0565 0.48 -0.5 584,370 - ompT b0565 0.49 -0.4 584,370 - ompT b0565 0.49 -0.7 584,371 - ompT b0565 0.49 -0.5 584,377 + ompT b0565 0.50 -1.1 584,391 + ompT b0565 0.51 +0.8 584,398 - ompT b0565 0.52 +0.0 584,399 - ompT b0565 0.52 -0.5 584,407 + ompT b0565 0.53 +1.8 584,415 + ompT b0565 0.54 +1.8 584,423 - ompT b0565 0.55 +1.1 584,423 - ompT b0565 0.55 -0.4 584,444 - ompT b0565 0.57 -0.5 584,492 - ompT b0565 0.62 +0.0 584,492 - ompT b0565 0.62 -1.1 584,494 - ompT b0565 0.62 +0.4 584,494 - ompT b0565 0.62 -0.1 584,500 - ompT b0565 0.63 +0.1 584,500 - ompT b0565 0.63 +0.8 584,516 + ompT b0565 0.64 +0.8 584,524 - ompT b0565 0.65 +0.0 584,524 - ompT b0565 0.65 -0.7 584,531 + ompT b0565 0.66 -1.4 584,537 + ompT b0565 0.66 +1.8 584,537 + ompT b0565 0.66 +0.2 584,537 + ompT b0565 0.66 +1.8 584,545 - ompT b0565 0.67 -1.2 584,564 - ompT b0565 0.69 -0.6 584,564 - ompT b0565 0.69 -0.2 584,565 - ompT b0565 0.69 -0.3 584,566 - ompT b0565 0.69 +0.6 584,566 - ompT b0565 0.69 -0.2 584,572 + ompT b0565 0.70 +0.5 584,572 + ompT b0565 0.70 -0.2 584,577 - ompT b0565 0.71 +0.6 584,580 + ompT b0565 0.71 -0.1 584,581 + ompT b0565 0.71 +0.8 584,582 + ompT b0565 0.71 +2.8 584,584 - ompT b0565 0.71 -0.1 584,587 + ompT b0565 0.72 -0.5 584,587 + ompT b0565 0.72 -1.9 584,589 + ompT b0565 0.72 -0.8 584,590 - ompT b0565 0.72 +0.0 584,608 - ompT b0565 0.74 -0.7 584,612 - ompT b0565 0.74 -0.3 584,612 - ompT b0565 0.74 -1.0
Or see this region's nucleotide sequence