Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frmB and frmA are separated by 93 nucleotides frmA and yaiN are separated by 34 nucleotides yaiN and yaiO are separated by 187 nucleotides
b0355: frmB - predicted esterase (NCBI), at 376,759 to 377,592
frmB
b0356: frmA - alcohol dehydrogenase class III/glutathione-dependent formaldehyde dehydrogenase (NCBI), at 377,686 to 378,795
frmA
b0357: yaiN - putative alpha helix chain (VIMSS), at 378,830 to 379,105
yaiN
b0358: yaiO - hypothetical protein (NCBI), at 379,293 to 380,066
yaiO
Position (kb) 377
378
379 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 376.692 kb on - strand at 376.719 kb on + strand at 376.745 kb on - strand at 376.745 kb on - strand at 376.747 kb on + strand at 376.755 kb on + strand at 376.771 kb on - strand at 376.783 kb on + strand at 376.853 kb on - strand, within frmB at 376.875 kb on + strand, within frmB at 376.906 kb on + strand, within frmB at 376.909 kb on + strand, within frmB at 376.909 kb on + strand, within frmB at 376.909 kb on + strand, within frmB at 376.927 kb on - strand, within frmB at 376.946 kb on - strand, within frmB at 377.044 kb on + strand, within frmB at 377.044 kb on + strand, within frmB at 377.052 kb on - strand, within frmB at 377.075 kb on + strand, within frmB at 377.075 kb on + strand, within frmB at 377.120 kb on - strand, within frmB at 377.120 kb on - strand, within frmB at 377.122 kb on + strand, within frmB at 377.375 kb on - strand, within frmB at 377.395 kb on - strand, within frmB at 377.400 kb on - strand, within frmB at 377.447 kb on + strand, within frmB at 377.499 kb on - strand, within frmB at 377.525 kb on + strand at 377.662 kb on - strand at 377.668 kb on + strand at 377.751 kb on + strand at 377.769 kb on - strand at 377.809 kb on + strand, within frmA at 377.859 kb on - strand, within frmA at 378.034 kb on - strand, within frmA at 378.051 kb on + strand, within frmA at 378.051 kb on + strand, within frmA at 378.135 kb on + strand, within frmA at 378.256 kb on + strand, within frmA at 378.256 kb on + strand, within frmA at 378.256 kb on + strand, within frmA at 378.283 kb on - strand, within frmA at 378.383 kb on - strand, within frmA at 378.383 kb on - strand, within frmA at 378.449 kb on + strand, within frmA at 378.479 kb on - strand, within frmA at 378.479 kb on - strand, within frmA at 378.484 kb on - strand, within frmA at 378.523 kb on - strand, within frmA at 378.626 kb on + strand, within frmA at 378.963 kb on - strand, within yaiN at 378.963 kb on - strand, within yaiN at 379.025 kb on + strand, within yaiN at 379.409 kb on - strand, within yaiO at 379.409 kb on - strand, within yaiO at 379.439 kb on - strand, within yaiO at 379.480 kb on - strand, within yaiO at 379.481 kb on - strand, within yaiO at 379.573 kb on + strand, within yaiO at 379.573 kb on + strand, within yaiO at 379.580 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.618 kb on + strand, within yaiO at 379.618 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.625 kb on + strand, within yaiO at 379.625 kb on + strand, within yaiO at 379.628 kb on + strand, within yaiO at 379.628 kb on + strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.770 kb on - strand, within yaiO at 379.779 kb on + strand, within yaiO at 379.779 kb on + strand, within yaiO at 379.787 kb on - strand, within yaiO at 379.787 kb on - strand, within yaiO at 379.788 kb on + strand, within yaiO
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 376,692 - -0.8 376,719 + +0.5 376,745 - -1.0 376,745 - -1.2 376,747 + +0.2 376,755 + +0.4 376,771 - -0.2 376,783 + -0.8 376,853 - frmB b0355 0.11 +0.3 376,875 + frmB b0355 0.14 +1.5 376,906 + frmB b0355 0.18 +0.2 376,909 + frmB b0355 0.18 -0.5 376,909 + frmB b0355 0.18 +0.2 376,909 + frmB b0355 0.18 +0.0 376,927 - frmB b0355 0.20 +1.8 376,946 - frmB b0355 0.22 -0.5 377,044 + frmB b0355 0.34 +1.5 377,044 + frmB b0355 0.34 -0.3 377,052 - frmB b0355 0.35 -0.5 377,075 + frmB b0355 0.38 -1.0 377,075 + frmB b0355 0.38 +0.7 377,120 - frmB b0355 0.43 -0.3 377,120 - frmB b0355 0.43 +0.2 377,122 + frmB b0355 0.44 -0.3 377,375 - frmB b0355 0.74 -0.3 377,395 - frmB b0355 0.76 -0.1 377,400 - frmB b0355 0.77 -0.3 377,447 + frmB b0355 0.82 +0.8 377,499 - frmB b0355 0.89 +0.5 377,525 + -0.1 377,662 - -0.7 377,668 + +0.4 377,751 + +0.6 377,769 - +0.1 377,809 + frmA b0356 0.11 -0.5 377,859 - frmA b0356 0.16 +0.2 378,034 - frmA b0356 0.31 -0.4 378,051 + frmA b0356 0.33 +0.1 378,051 + frmA b0356 0.33 +0.8 378,135 + frmA b0356 0.40 -0.2 378,256 + frmA b0356 0.51 +0.5 378,256 + frmA b0356 0.51 +1.0 378,256 + frmA b0356 0.51 -0.6 378,283 - frmA b0356 0.54 +0.3 378,383 - frmA b0356 0.63 -1.5 378,383 - frmA b0356 0.63 +0.2 378,449 + frmA b0356 0.69 -1.3 378,479 - frmA b0356 0.71 +0.1 378,479 - frmA b0356 0.71 +0.2 378,484 - frmA b0356 0.72 +0.4 378,523 - frmA b0356 0.75 -0.4 378,626 + frmA b0356 0.85 -1.2 378,963 - yaiN b0357 0.48 -0.8 378,963 - yaiN b0357 0.48 -1.2 379,025 + yaiN b0357 0.71 +0.2 379,409 - yaiO b0358 0.15 -0.6 379,409 - yaiO b0358 0.15 +0.1 379,439 - yaiO b0358 0.19 +0.1 379,480 - yaiO b0358 0.24 -0.0 379,481 - yaiO b0358 0.24 -0.0 379,573 + yaiO b0358 0.36 -0.1 379,573 + yaiO b0358 0.36 -0.9 379,580 + yaiO b0358 0.37 -0.1 379,616 + yaiO b0358 0.42 +0.3 379,616 + yaiO b0358 0.42 -1.9 379,616 + yaiO b0358 0.42 +0.2 379,616 + yaiO b0358 0.42 -0.4 379,616 + yaiO b0358 0.42 +0.4 379,616 + yaiO b0358 0.42 -0.8 379,616 + yaiO b0358 0.42 -0.2 379,616 + yaiO b0358 0.42 -0.4 379,618 + yaiO b0358 0.42 -0.2 379,618 + yaiO b0358 0.42 +0.8 379,621 + yaiO b0358 0.42 +1.2 379,621 + yaiO b0358 0.42 -0.2 379,621 + yaiO b0358 0.42 -0.3 379,621 + yaiO b0358 0.42 -1.3 379,621 + yaiO b0358 0.42 +0.0 379,621 + yaiO b0358 0.42 +1.2 379,625 + yaiO b0358 0.43 +0.4 379,625 + yaiO b0358 0.43 +0.6 379,628 + yaiO b0358 0.43 +0.6 379,628 + yaiO b0358 0.43 -2.6 379,629 - yaiO b0358 0.43 -1.4 379,629 - yaiO b0358 0.43 +0.8 379,629 - yaiO b0358 0.43 -0.0 379,629 - yaiO b0358 0.43 +0.0 379,629 - yaiO b0358 0.43 -0.1 379,629 - yaiO b0358 0.43 +0.6 379,770 - yaiO b0358 0.62 +2.8 379,779 + yaiO b0358 0.63 -0.1 379,779 + yaiO b0358 0.63 -1.0 379,787 - yaiO b0358 0.64 +0.8 379,787 - yaiO b0358 0.64 +0.2 379,788 + yaiO b0358 0.64 +0.8
Or see this region's nucleotide sequence